天津医药

天津医药 ›› 2020, Vol. 48 ›› Issue (9): 839-842.doi: 10.11958/20200487

• 实验研究 • 上一篇    下一篇

茵陈蒿汤调节胆汁酸代谢并干预阻塞性黄疸大鼠肝细胞线粒体DNA损伤的研究

刘军舰1，2，李忠廉2△，尚海涛2   

  1. 1天津中医药大学（邮编301617）；2天津市南开医院
  • 收稿日期:2020-03-13 修回日期:2020-07-09 出版日期:2020-09-15 发布日期:2020-09-15
  • 通讯作者: 李忠廉 E-mail:nkyylzl@163.com
  • 作者简介:刘军舰（1987），男，博士在读，主治医师，主要从事肝胆胰腺疾病中西医结合相关研究
  • 基金资助:
    天津市中医药重点领域科技项目（2019003）

Study on Yinchenhao decoction regulating bile acid metabolism and intervening the mitochondrial DNA damage in liver cells of rats with obstructive jaundice

LIU Jun-jian1, 2, LI Zhong-lian2△, SHANG Hai-tao2   

  1. 1 Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China; 2 Tianjin Nankai Hospital
  • Received:2020-03-13 Revised:2020-07-09 Published:2020-09-15 Online:2020-09-15

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摘要： 摘要：目的　探讨茵陈蒿汤调节胆汁酸代谢对阻塞性黄疸大鼠肝细胞线粒体DNA（mtDNA）损伤的影响及其意义。方法　SPF级雄性成年SD大鼠54只，随机分为假手术组、模型组、茵陈蒿汤组，每组18只。梗阻建模第3天后，模型组每日给予生理盐水灌胃，茵陈蒿汤组每日给予茵陈蒿汤灌胃，各组观察时相点为建模后第1、3、8天，每时相点处死大鼠6只。检测血总胆红素（TBIL）、直接胆红素（DBIL）、丙氨酸转氨酶（ALT）、γ-谷氨酰转肽酶（GGT）、总胆汁酸（TBA），尿TBA及尿量变化情况，实时荧光定量PCR法检测肝细胞mtDNA的相对量。结果　给予茵陈蒿汤治疗的大鼠，循环血中胆汁酸含量降低（P＜0.05），尿量及尿胆汁酸含量均增加（P＜0.05）。模型组与茵陈蒿汤组mtDNA的相对量均较假手术组减少，茵陈蒿汤组mtDNA的相对量第8天较第3天回升。结论　茵陈蒿汤可能通过肝-肾轴途径调节胆汁酸代谢以减少肝细胞mtDNA损伤，进而改善阻塞性黄疸大鼠肝功能。

关键词: 胆汁淤积；黄疸, 阻塞性；胆红素；胆汁酸类和盐类；DNA, 线粒体；茵陈蒿汤；肝疾病

Abstract: Abstract: Objective　To investigate the effect of Yinchenhao decoction on mitochondrial DNA (mtDNA) damage of hepatocytes by the regulation of bile acid metabolism in rats with obstructive jaundice. Methods　A total of 54 SPF male adult SD rats were selected and divided into 3 groups randomly, including the sham operation group, the model group and the Yinchenhao decoction group. Three days after building the obstruction modeling, the model group was given a gavage of normal saline every day, while the Yinchenhao decoction group was given a gavage of Yinchenhao decoction. The observation time points of each group were the first, third, and eighth day after modeling. Six rats were killed at each rated point to detect the changes of total bilirubin (TBIL), direct bilirubin (DBIL), alanine aminotransferase (ALT), glutamyl transpeptidase (GGT), total bile acid (TBA) in blood, TBA in urine and urine volume. The relative amount of mtDNA in hepatocytes was detected by quantitative PCR. Results　The content of circulating blood bile acid decreased, and the urine volume and urine bile acid increased in rats treated with Yinchenhao Decoction (P＜0.05). The relative amount of mtDNA decreased in the mode group and Yinchenhao decoction group compared with that of the sham operation group. The  relative amount of mtDNA in the Yinchenhao decoction group increased on the 8th day compare with that on the 3rd day. Conclusion　Yinchenhao decoction can improve the function of hepatocytes by regulating bile acid metabolism through the liver-kidney axis to reduce liver cell mtDNA damage in rats with obstructive jaundice. 

Key words: cholestasis, jaundice, obstructive, bilirubin, bile acids and salts, DNA, mitochondrial, Yinchenhao Decoction, liver diseases

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