天津医药

天津医药 ›› 2021, Vol. 49 ›› Issue (4): 337-341.doi: 10.11958/20202591

• 细胞与分子生物学 •    下一篇

螺旋藻多糖对急性T淋巴细胞白血病细胞的杀伤作用

李岩，张竞男，张谨，石文华，叶书梅，邓秀玲，扈瑞平，薛慧婷△   

  1. 内蒙古医科大学基础医学院附属医院（邮编010110）
  • 收稿日期:2020-09-16 修回日期:2021-01-21 出版日期:2021-04-15 发布日期:2021-04-16
  • 通讯作者: 薛慧婷 E-mail:huiting410@hotmail.com
  • 作者简介:李岩（1987），女，硕士，主治医师，主要从事血液系统肿瘤研究。E-mail：413271439@qq.com
  • 基金资助:
    国家自然科学基金项目（32060217）；内蒙古自然科学基金项目（2019BS08013）；内蒙古自治区高等学校科学研究项目（NJZY19100）；内蒙古科技计划（2019GG160）；内蒙古蒙医药协同创新项目（MYYXT201901）

Cytotoxic effects of spiruline platensis polysaccharides on acute T lymphocyte leukemia

LI Yan, ZHANG Jing-nan, ZHANG Jin, SHI Wen-hua, YE Shu-mei, DENG Xiu-ling, HU Rui-ping, XUE Hui-ting△   

  1. College of Basic Medicine, Inner Mongolia Medical University Affiliated Hospital, Hohhot 010110, China
  • Received:2020-09-16 Revised:2021-01-21 Published:2021-04-15 Online:2021-04-16
  • Contact: XUE Hui-ting E-mail:huiting410@hotmail.com

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摘要： 目的　研究螺旋藻多糖（SPP）对人急性T淋巴细胞白血病细胞系Jurkat的杀伤作用。方法　取对数生长期Jurkat细胞，分为对照组和1 g/L、2 g/L、5 g/L SPP组。采用MTT法检测SPP对Jurkat细胞增殖的影响，Western blot法检测凋亡蛋白caspase-3、细胞膜表面受体CD45、CD71以及细胞内部信号分子p-ERK、p-JNK、t-ERK和t-JNK蛋白表达水平。结果　1 g/L、2 g/L和5 g/L SPP组细胞增殖抑制率依次升高，分别为53.286%±3.112%、80.075%±4.240%和86.430%±4.614%。2 g/L、5 g/L SPP组cleaved caspase-3蛋白表达水平高于对照组，5 g/L SPP组高于1 g/L SPP组（P＜0.05）。1 g/L、2 g/L SPP组CD45蛋白表达水平高于对照组（P＜0.05），CD71蛋白表达水平与对照组差异无统计学意义；5 g/L SPP组CD45蛋白表达水平均高于对照组、1 g/L和2 g/L SPP组；CD71蛋白表达水平低于对照组（P＜0.05），与1 g/L和2 g/L SPP组差异无统计学意义。对照组、1 g/L SPP组、2 g/L SPP组和5 g/L SPP组p-ERK蛋白表达水平依次升高，组间多重比较差异均有统计学意义；各组间t-ERK、p-JNK和t-JNK蛋白表达水平差异均无统计学意义。结论　SPP对Jurkat细胞具有杀伤作用，其机制可能是SPP通过影响细胞膜表面受体CD45和CD71的表达，将凋亡信号传递到细胞内部，通过活化ERK，最终活化caspase-3，诱导细胞凋亡。

关键词: 螺旋藻属, 多糖, 白血病, Jurkat细胞, 细胞凋亡, 细胞外信号调节MAP激酶类

Abstract: Objective　To investigate the cytotoxic effect of spirulina platensis polysaccharides (SPP) on acute T lymphocyte leukemia Jurkat cells. Methods　SPP was obtained by water extraction and ethanol precipitation. The effect of SPP on cell proliferation was determined by MTT assay. Caspase-3 was tested by Western blot assay. Furthermore, the cell surface expression of CD45 and CD71, and the expression levels of p-ERK, p-JNK, t-ERK and t-JNK were also evaluated. Results　SPP inhibited Jurkat cell proliferation in a dose-dependent manner (1 g/L, 2 g/L and 5 g/L), the inhibitory rates were 53.286%±3.112%, 80.075%±4.240% and 86.430%±4.614%, respectively. The expression levels of cleaved caspase-3 were higher in 2 g/L and 5 g/L SPP groups than those in the control group, and that in 5 g/L SPP group was higher than that in 1 g/L SPP group (P＜0.05). The expression levels of CD45 were higher in 1 g/L and 2 g/L SPP groups than those in the control group (P＜0.05), while there was no significant difference in the expression level of CD71 between the two groups. The expression levels of CD45 were higher in 5 g/L SPP group than those in control group, 1 g/L SPP group and 2 g/L SPP group. The expression level of CD71 was lower in 5 g/L SPP group than that in control group (P＜0.05), and there was no significant difference in the expression level of CD71 between 1 g/L and 2 g/L SPP groups. The expression levels of p-ERK were increased significantly in turn in the control group, 1 g/L, 2 g/L and 5 g/L SPP groups. There were no significant differences in expression levels of t-ERK, p-JNK and t-JNK between groups. Conclusion　SPP could inhibit Jurkat cell growth and induce cell death, which might through affecting CD45 and CD71 expression on cell surface, then activating ERK and finally activating caspase-3 to induce cell apoptosis.

Key words: spirulina, polysaccharide, leukemia, Jurkat cells, apoptosis, extracellular signal-regulated MAP kinases

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