天津医药

天津医药 ›› 2022, Vol. 50 ›› Issue (6): 595-600.doi: 10.11958/20212614

• 实验研究 • 上一篇    下一篇

大鼠缺血性脑卒中早期miR-27a表达与铁死亡的关系

张婧，孙辉，朱礼军，冯子人，杜琳，孟爱国△   

  1. 华北理工大学附属医院检验科，唐山市医学分子检验与诊断重点实验室（邮编063000）
  • 收稿日期:2021-11-24 修回日期:2022-03-05 出版日期:2022-06-15 发布日期:2023-12-20
  • 通讯作者: 孟爱国 E-mail:magmaster@sina.com
  • 基金资助:
    河北省高等学校科学技术研究项目（ZD2019050）；2021年河北省政府资助临床医学人才培养项目（冀卫办科教［2021］9号）

Relationship between miR-27a expression and ferroptosis in the early stage of ischemic stroke of rats

ZHANG Jing, SUN Hui, ZHU Lijun, FENG Ziren, DU Lin, MENG Aiguo△   

  1. North China University of Science and Technology Affiliated Hospital, Key Laboratory of Medical Molecular Testing and Diagnosis in Tangshan, Tangshan 063000, China
  • Received:2021-11-24 Revised:2022-03-05 Published:2022-06-15 Online:2023-12-20

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摘要： 摘要：目的　探讨miR-27a在缺血性脑卒中早期的表达及其与铁死亡的关系。方法　88只SD大鼠采用随机数字表法进行分组。（1）正常（Control）组、假手术（Sham）组、模型组（按照缺血时间分为6、12、24和48 h组）；（2）缺血48 h组（I 48 h组）、缺血48 h+Ferrostatin-1组（I 48 h+Fer-1组）、缺血48 h+DMSO组（I 48 h+DMSO组）；（3）缺血48 h组（I 48 h组）、缺血48 h+阴性对照组（I 48 h+NC组）、缺血48 h+miR-27a激动剂组（I 48 h+ago-miR-27a组）、缺血48 h+miR-27a抑制剂组（I 48 h+antago-miR-27a组）。分组处理结束后分离脑组织，TTC染色观察脑梗死面积，Western blot检测谷胱甘肽过氧化物酶4（GPX4）蛋白表达情况，试剂盒检测谷胱甘肽（GSH）、组织铁、丙二醛（MDA）含量；qPCR检测miR-27a表达情况。结果　（1）与Sham组相比，各缺血组miR-27a表达、铁和MDA含量升高，而GPX4和GSH表达降低（P＜0.05），以缺血48 h为著。（2）与I 48 h+DMSO组相比，I 48 h+Fer-1组脑梗死面积减小，GPX4和GSH表达升高，而铁和MDA含量减少（P＜0.05）。（3）与I 48 h+NC组相比，I 48 h+ago-miR-27a组中大鼠脑梗死面积及miR-27a表达增加，GPX4和GSH表达降低，铁和MDA含量增多（P＜0.05）；而antago-miR-27a组上述指标均出现相反变化。结论　miR-27a在大鼠缺血性脑卒中早期表达升高，可通过促进铁死亡的发生而加重缺血后脑组织损伤。

关键词: 卒中；大鼠, Sprague-Dawley；miR-27a；铁死亡

Abstract: Abstract: Objective　To investigate the expression of miR-27a in the early stage of ischemic stroke in rats and its relationship with ferroptosis. Methods　A total of 88 SD rats were randomly divided into (1) the control group, the sham group, the ischemia group (6, 12, 24 and 48 h groups according to ischemia time); (2) the ischemia 48 h group (I 48 h group), the solvent control group (I 48 h+DMSO group), the ischemia 48 h+Ferrostatin-1 group (I 48 h+Fer-1 group); (3) the I 48 h+negative control (I 48 h+NC) group, the I 48 h+miR-27a agonist group (I 48 h+ago-miR-27a group) and the I 48 h+miR-27a antagonist group (I 48 h+antago-miR-27a group). The brain tissue was separated after treatment. TTC staining was used to observe the size of cerebral infarction. The expression of glutathione peroxidase 4 (GPX4) was analyzed by Western blot assay. The glutathione (GSH), iron and methylene dioxyamphetamine (MDA) contents were detected by detection kits. The expression of miR-27a was analyzed by qPCR. Results　(1) Compared with the sham group, the expression of miR-27a and the contents of iron and MDA were increased in the ischemia group, while the expressions of GPX4 and GSH were decreased (P＜0.05), which were the most significant at 48 h of ischemia. (2) Compared with the I 48 h+DMSO group, the cerebral infarct size was decreased in the I 48 h+Fer-1 group, the expression levels of GPX4 and GSH were increased, while the contents of iron and MDA were decreased (P＜0.05). (3) Compared with the I 48 h+NC group, the cerebral infarct area and miR-27a expression were increased in the I 48 h+ago-miR-27a group, while the expressions of GPX4 and GSH were decreased, and the contents of iron and MDA were increased (P＜0.05). However, the above parameters showed opposite changes in the antago-miR-27a group. Conclusion　The increased expression of miR-27a in the early stage of ischemic stroke in rats can aggravate the damage of ischemic brain injury by promoting the occurrence of ferroptosis.

Key words: stroke, rats, Sprague-Dawley, miR-27a, ferroptosis