天津医药

天津医药 ›› 2023, Vol. 51 ›› Issue (5): 460-463.doi: 10.11958/20221264

• 细胞与分子生物学 • 上一篇    下一篇

麝香保心丸对缺氧复氧心肌细胞的保护作用及凋亡的影响

魏娜1(), 李思源1, 高苑1, 王雪瑞2, 肖斌3, 牛艺璇3, 杨文霞2, 刘振兵4,△()   

  1. 1.内蒙古医科大学研究生院（邮编010100）
    2.东胜区人民医院超声科
    3.内蒙古医科大学鄂尔多斯临床医学院鄂尔多斯市中心医院临床药学实验室，4心血管内科二区
  • 收稿日期:2022-08-15 修回日期:2022-11-21 出版日期:2023-05-15 发布日期:2023-05-05
  • 通讯作者: △E-mail:LZHBL44@126.com
  • 作者简介:魏娜(1994)，女，硕士在读，主要从事心血管内科方面研究。E-mail：1255711789@qq.com
  • 基金资助:
    国家自然科学基金资助项目(81860053);中华中医药学会委托课题(2022HH-013)

Protective effect of Shexiang Baoxin Pill on hypoxia-reoxygenation cardiomyocytes and its effect on apoptosis

WEI Na1(), LI Siyuan1, GAO Yuan1, WANG Xuerui2, XIAO Bin3, NIU Yixuan3, YANG Wenxia2, LIU Zhenbing4,△()   

  1. 1. Graduate School of Inner Mongolia Medical University, Hohhot 010100, China
    2. Department of Ultrasound, Dongsheng People's Hospital
    3. Clinical Pharmacy Laboratory, 4 Area 2 of Cardiovascular Medicine, Ordos Clinical Medical College of Inner Mongolia Medical University Ordos Central Hospital
  • Received:2022-08-15 Revised:2022-11-21 Published:2023-05-15 Online:2023-05-05
  • Contact: △E-mail:LZHBL44@126.com

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摘要：

目的 探究麝香保心丸（SBP）对缺氧复氧心肌细胞是否具有保护作用及对调亡的影响。方法 建立心肌细胞缺氧-复氧模型。用水提法提取SBP的活性成分。将缺氧复氧心肌细胞随机分为对照组、模型组、0.5 g/L SBP组、1 g/L SBP组、2 g/L SBP组和5 g/L SBP组。用CCK-8检测各组细胞存活率，SBP干预组选择存活率最高的组别进行后续实验。采用Anexxin-V FITC/PI双染色法检测各组细胞凋亡率。采用Western blot检测各组细胞B细胞淋巴瘤因子2（Bcl-2）、Bcl-2相关X蛋白（Bax）、胱天蛋白酶-3（Caspase-3）蛋白相对表达水平。结果 与模型组比较，0.5 g/L、1 g/L、2 g/L、5 g/L SBP组的心肌细胞存活率均有升高（P<0.01），且2 g/L SBP组心肌细胞存活率最高。与对照组比较，模型组和2 g/L SBP组凋亡率及Bax、Caspase-3相对表达水平升高（P<0.05），而Bcl-2差异无统计学意义；与模型组比较，2 g/L SBP组凋亡率及Bax、Caspase-3相对表达水平下降（P<0.05），而Bcl-2相对表达水平和Bcl-2/Bax比值升高（P<0.05）。结论 麝香保心丸对缺氧复氧心肌细胞具有保护作用，抑制细胞凋亡是其保护机制之一。

关键词: 麝香保心丸, 肌细胞, 心脏, 心肌再灌注损伤, 细胞凋亡, 细胞保护

Abstract:

Objective To study the protective effect of Shexiang Baoxin Pill (SBP) on hypoxia-reoxygenation cardiomyocytes and its effect on apoptosis. Methods The model of hypoxia and reoxygenation of cardiomyocytes was established. The active components of SBP were extracted by water extraction. According to whether different concentrations of SBP active components were given, cells were divided into the control group, the model group, the 0.5 g/L SBP group, the 1 g/L SBP group, the 2 g/L SBP group and the 5 g/L SBP group. The highest survival rate in the SBP intervention group was selected as the group for the follow-up experiments. The cell survival rate of each group was detected by CCK-8. The apoptosis rate of each group was detected by Anexxin-VFITC/PI double staining. Western bolt assay was used to detect the relative expression levels of B cell lymphoma factor 2 (Bcl-2), Bcl-2 associated X protein (Bax) and cysteine protease 3 (Caspase-3) in each group. Results Compared with the model group, survival rates of cardiomyocytes were increased in the 0.5 g/L, 1 g/L, 2 g/L and 5 g/L SBP groups (P<0.01), and the survival rate of cardiomyocytes was the highest in the 2 g/L SBP group. Compared with the control group, the apoptosis rate, relative expression levels of Bax and Caspase-3 were increased in the model group and the 2 g/L SBP group (P<0.05), but there was no significant difference in Bcl-2 between the groups. Compared with the model group, the apoptosis rate, relative expression levels of Bax and Caspase-3 were decreased in the 2 g/L SBP group (P<0.05), while relative expression levels of Bcl-2 and Bcl-2/Bax ratio were increased (P<0.05). Conclusion Shexiang Baoxin Pill has a protective effect on hypoxia-reoxygenation cardiomyocytes, and the inhibition of apoptosis is one of its protective mechanisms.

Key words: Shexiang Baoxin Pill, myocytes, cardiac, myocardial reperfusion injury, apoptosis, cytoprotection

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