硫化铼纳米颗粒在能谱CT成像及光热治疗中的应用研究

doi:10.11958/20230209

天津医药 ›› 2023, Vol. 51 ›› Issue (8): 883-887.doi: 10.11958/20230209

硫化铼纳米颗粒在能谱CT成像及光热治疗中的应用研究

王笑一¹(), 王欣宇², 张雪宁³^,^△(), 李敬¹

1 天津医科大学第二医院超声科（邮编300211）
2 天津医科大学总医院医学影像科
3 天津医科大学第二医院医学影像科

收稿日期:2023-02-21 修回日期:2023-03-28 出版日期:2023-08-15 发布日期:2023-08-10
通讯作者: ^△E-mail：luckyxn@126.com
作者简介:王笑一（1993），男，住院医师，主要从事纳米探针在肿瘤诊疗一体化方向的基础及应用方面研究。E-mail：wangxy@tmu.edu.cn
基金资助:
天津市教委科研计划项目（自然科学）(2021KJ229)

Study of rhenium sulfide nanoparticles in spectral CT imaging and photothermal therapy

WANG Xiaoyi¹(), WANG Xinyu², ZHANG Xuening³^,^△(), LI Jing¹

1 Department of Ultrasound, the Second Hospital of Tianjin Medical University, Tianjin 300211, China
2 Department of Radiology, Tianjin Key Laboratory of Functional Imaging, Tianjin Medical University General Hospital
3 Department of Radiology, the Second Hospital of Tianjin Medical University

Received:2023-02-21 Revised:2023-03-28 Published:2023-08-15 Online:2023-08-10
Contact: ^△E-mail: luckyxn@126.com

王笑一, 王欣宇, 张雪宁, 李敬. 硫化铼纳米颗粒在能谱CT成像及光热治疗中的应用研究[J]. 天津医药, 2023, 51(8): 883-887.

WANG Xiaoyi, WANG Xinyu, ZHANG Xuening, LI Jing. Study of rhenium sulfide nanoparticles in spectral CT imaging and photothermal therapy[J]. Tianjin Medical Journal, 2023, 51(8): 883-887.

摘要/Abstract

摘要：

目的探讨硫化铼（ReS₂）纳米颗粒的能谱CT成像性能及其在乳腺癌细胞中的光热治疗效果。方法采用一锅法在室温下合成ReS₂纳米颗粒；利用X射线光电子能谱、高分辨透射电镜、紫外吸收光谱对ReS₂纳米颗粒进行材料学基本性质的表征。比较等效浓度的ReS₂纳米颗粒和碘海醇在不同能量下（60~140 keV）的能谱CT成像能力。通过测定不同质量浓度的ReS₂纳米颗粒水溶液（0、50、100、250和500 mg/L）在体外光热升温效果来评估纳米颗粒的光热转换能力。取小鼠乳腺癌细胞4T1与不同质量浓度的ReS₂纳米颗粒水溶液（0、20、40、80和100 mg/L）共孵育，并通过MTT实验评估纳米颗粒的细胞毒性；通过细胞荧光染色法可视化观察分析ReS₂纳米颗粒的光热细胞杀伤效果。结果高分辨透射电镜观察到制备的纳米颗粒粒径<10 nm，紫外吸收光谱证实ReS₂在808 nm处具有较强的吸收能力。当能量在60~140 keV时，等效浓度下ReS₂纳米颗粒的CT成像效果明显优于碘海醇溶液。在功率密度为3 W/cm²的808 nm激光照射下，500 mg/L ReS₂溶液的温度可提高44.90 °C±1.2 °C，而在相同条件下，纯水的温度仅提高了9.27 °C±0.74 °C。细胞学实验表明，在无激光照射的情况下，40~100 mg/L的ReS₂纳米颗粒处理后，细胞存活率均>85%；在激光照射下，ReS₂纳米颗粒浓度>40 mg/L时，生存率已<30%。结论 ReS₂纳米颗粒制备简单，具有良好的生物相容性和光热治疗性能，且能谱CT成像效果显著，极具临床转化潜力。

关键词: 造影剂, 纳米粒子, CT成像, 硫化铼, 光热治疗

Abstract:

Objective To investigate the spectral CT imaging performance of Rhenium sulfide (ReS₂) nanoparticles and their effect on photothermal therapy in breast cancer cells. Methods ReS₂ nanoparticles were synthesized by one-pot method at room temperature. The basic materialistic properties of ReS₂ nanoparticles were characterized by X-ray photoelectron spectroscopy, high-resolution transmission electron microscopy and UV absorption spectroscopy. The spectral CT imaging capability of ReS₂ nanoparticles with the same molar concentration of iodine at different energies (60-140 keV) was compared. The photothermal conversion ability of nanoparticles was evaluated by measuring the in vitro photothermal warming of aqueous solutions of ReS₂ nanoparticles with different mass concentrations (0、20、40、80 and 100 mg/L). Mouse breast cancer cells 4T1 were co-incubated with different mass concentrations of ReS₂ nanoparticle aqueous solution (0, 20, 40, 80 and 100 mg/L). The cytotoxicity of nanoparticles was evaluated by MTT experiment and cell photothermal killing experiment. The photothermal cell killing effect of ReS₂ nanoparticles was visually observed and analyzed by cytofluorescent staining. Results High-resolution transmission electron microscopy observed that the particle size of the prepared nanoparticles was less than 10 nm. The UV absorption spectrum confirmed the strong absorption of ReS₂ at 808 nm. When the energy was in the range of 60-140 keV, the CT imaging effect of ReS₂ nanoparticles at equivalent molar concentration was significantly better than that of iohexol solution. The temperature of 500 mg/L ReS₂ solution could be increased by 44.90 °C ± 1.2 °C under 808 nm laser irradiation with a power density of 3 W/cm², while the temperature of pure water was only increased by 9.27 °C ± 0.74 °C under the same conditions. Cellular experiments showed that in the absence of laser irradiation, the survival rate of 4T1 cells treated with ReS₂ nanoparticles at concentrations of 40-100 mg/L were all greater than 85%. The cell survival rate was also reduced at ReS₂ nanoparticle concentrations of 40-100 mg/L compared to the 0-40 mg/L case under laser irradiation, but the survival rate was already less than 30%. Conclusion ReS₂ nanoparticles are simple to prepare, have good biocompatibility and photothermal therapeutic properties, and have strong killing effect on breast cancer cells under NIR laser irradiation, which have good clinical translation potential.

Key words: contrast media, nanoparticles, CT imaging, rhenium sulfide, photothermal therapy

中图分类号:

R817.12

图/表 8

图1 ReS2纳米颗粒的X射线光电子能谱图

Fig.1 X-ray photoelectron spectra of ReS2 nanoparticles

图2 ReS2纳米颗粒的高分辨率透射电子显微镜图

Fig.2 High resolution transmission microscope image of ReS2 nanoparticles

图3 ReS2在808 nm近红外光的浓度-吸收强度标准曲线

Fig.3 The standard curve of absorbance at 808 nm versus ReS2 concentration

图4 等效浓度（35 mmol/L的Re和I元素浓度）的ReS2和碘海醇在不同能量下的CT影像

Fig.4 The monochromatic spectral CT images of ReS2 (35 mmol/L Re) and iohexol (35 mmol/L I) at different monochromatic energies

图5 等效浓度（35 mmol/L的Re和I元素浓度）的ReS2和碘海醇在不同能量下的CT值

Fig.5 The HU values of ReS2 (35 mmol/L Re) and iohexol (35 mmol/L I) at different monochromatic energies

图6 不同质量浓度的ReS2纳米颗粒光热升温热值图

Fig.6 The infrared images of pure water and ReS2 nanoparticles with concentrations of 50, 100, 250, 500 mg/L under a laser irradiation.

表1 无激光照射组和激光照射组在不同ReS2浓度下的细胞存活率（n=3，%，$\bar{x}±s$）

Tab.1 Cell survival rate of non-laser irradiation group and laser irradiation group at different ReS2 concentrations

组别	ReS₂浓度					F
组别	0 mg/L	20 mg/L	40 mg/L	80 mg/L	100 mg/L	F
无激光照射组	97.73±3.67	95.23±4.12	87.29±3.28^A	89.45±2.54^A	86.63±1.37^A	4.951^＊
激光照射组	106.30±3.24	100.79±2.37	34.61±1.74^A	18.59±2.02^A	16.19±0.38^A	852.241^＊＊

图7 无激光和激光照射下不同浓度ReS2纳米颗粒处理后4T1细胞的存活情况

Fig.7 Survival of 4T1 cells treated with ReS2 nanoparticles at different concentrations without laser and under laser irradiation

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硫化铼纳米颗粒在能谱CT成像及光热治疗中的应用研究

Study of rhenium sulfide nanoparticles in spectral CT imaging and photothermal therapy

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