LncRNA TUG1通过调节miR-181b-5p/PDCD4轴对高糖诱导的心肌细胞凋亡的影响

doi:10.11958/20230523

天津医药 ›› 2023, Vol. 51 ›› Issue (12): 1281-1287.doi: 10.11958/20230523

• 细胞与分子生物学 • 下一篇

LncRNA TUG1通过调节miR-181b-5p/PDCD4轴对高糖诱导的心肌细胞凋亡的影响

吕朝阳¹(), 黄婷¹, 徐在革¹, 刘惠双²^,^△(), 杨营军¹, 李真真¹, 敖文¹

1.郑州市第七人民医院内分泌科（邮编450016）
2.郑州市第七人民医院营养科（邮编450016）

收稿日期:2023-04-17 修回日期:2023-07-03 出版日期:2023-12-15 发布日期:2023-12-22
通讯作者: ^△ E-mail：15803870664@139.com
作者简介:吕朝阳（1988），女，主治医师，主要从事内分泌与代谢病方面研究。E-mail：cyqueen88@163.com
基金资助:
河南省医学科技攻关计划联合共建项目(LHGJ20191124)

Impact of LncRNA TUG1 on high glucose-induced cardiomyocyte apoptosis by regulating the miR-181b-5p/PDCD4 axis

LYU Chaoyang¹(), HUANG Ting¹, XU Zaige¹, LIU Huishuang²^,^△(), YANG Yingjun¹, LI Zhenzhen¹, AO Wen¹

1. Department of Endocrinology, the 7th People's Hospital of Zhengzhou, Zhengzhou 450016, China
2. Department of Nutrition, the 7th People's Hospital of Zhengzhou, Zhengzhou 450016, China

Received:2023-04-17 Revised:2023-07-03 Published:2023-12-15 Online:2023-12-22
Contact: ^△ E-mail：15803870664@139.com

吕朝阳, 黄婷, 徐在革, 刘惠双, 杨营军, 李真真, 敖文. LncRNA TUG1通过调节miR-181b-5p/PDCD4轴对高糖诱导的心肌细胞凋亡的影响[J]. 天津医药, 2023, 51(12): 1281-1287.

LYU Chaoyang, HUANG Ting, XU Zaige, LIU Huishuang, YANG Yingjun, LI Zhenzhen, AO Wen. Impact of LncRNA TUG1 on high glucose-induced cardiomyocyte apoptosis by regulating the miR-181b-5p/PDCD4 axis[J]. Tianjin Medical Journal, 2023, 51(12): 1281-1287.

摘要/Abstract

摘要：

目的探讨长链非编码RNA（LncRNA）牛磺酸上调基因1（TUG1）通过调节miR-181b-5p/程序性细胞死亡蛋白4（PDCD4）轴对高糖诱导的心肌细胞凋亡的影响。方法采用高糖（25 mmol/L葡萄糖）在体外构建糖尿病心肌病（DCM）细胞模型。AC16细胞分为NG组、HG组、HG+sh-NC组、HG+sh-TUG1组、HG+miR-NC组、HG+miR-181b-5p组、HG+sh-TUG1+anti-miR-NC组、HG+sh-TUG1+anti-miR-181b-5p组、HG+miR-181b-5p+pcDNA组、HG+miR-181b-5p+pc-PDCD4组。细胞计数试剂盒-8（CCK-8）法检测细胞活力；乳酸脱氢酶（LDH）测定试剂盒检测LDH释放总量；采用实时定量聚合酶链反应（qRT-PCR）检测TUG1、miR-181b-5p和PDCD4 mRNA表达；流式细胞术检测细胞凋亡；Western blot检测B细胞淋巴瘤2-相关X（Bax）、活化的胱天蛋白酶3（cleaved caspase 3）和PDCD4蛋白表达；caspase-Glo3检测试剂盒评估caspase 3活性；双萤光素酶报告基因实验验证TUG1或PDCD4与miR-181b-5p的靶向关系。结果与NG组比较，HG组细胞活性降低，LDH释放总量、凋亡率、Bax、cleaved caspase 3表达及caspase 3活性升高（P<0.05），敲低TUG1或上调miR-181b-5p表达可拮抗上述变化（P<0.05）。抑制miR-181b-5p可减轻TUG1沉默对高糖处理下心肌细胞活力和凋亡的影响（P<0.05）。PDCD4过表达可减弱miR-181b-5p上调对高糖处理的心肌细胞活力的促进作用和对凋亡的抑制作用。TUG1可通过吸附miR-181b-5p上调PDCD4表达（P<0.05）。结论 TUG1通过下调miR-181b-5p、上调PDCD4表达促进高糖诱导的心肌细胞凋亡。

关键词: 牛磺酸上调基因1, miR-181b-5p, 程序性细胞死亡蛋白4, 高糖, 心肌细胞, 凋亡

Abstract:

Objective To investigate the impact of long non-coding RNA (LncRNA) taurine up-regulated gene 1 (TUG1) on high glucose-induced cardiomyocyte apoptosis by regulating miR-181b-5p/programmed cell death protein 4 (PDCD4) axis. Methods Diabetic cardiomyopathy (DCM) cell model was established in vitro with high glucose (HG,25 mmol/L glucose). AC16 cells were divided into the NG (5.5 mmol/L glucose) group, the HG group, the HG+sh-NC group, the HG+sh-TUG1 group, the HG+miR-NC group, the HG+miR-181b-5p group, the HG+sh-TUG1+anti-miR-NC group, the HG+sh-TUG1+anti-miR-181b-5p group, the HG+miR-181b-5p+pcDNA group and HG+miR-181b-5p+pc-PDCD4 group. The Cell Counting Kit-8 (CCK-8) method was applied to detect cell viability. Lactate dehydrogenase (LDH) assay was applied to detect LDH release. Quantitative real-time polymerase chain reaction (qRT-PCR) was applied to detect expression levels of TUG1, miR-181b-5p and PDCD4 mRNA. Flow cytometry was applied to detect apoptosis. Western blot assay was applied to detect levels of B-cell lymphoma 2-associated X (Bax), activated caspase 3 (cleaved caspase 3) and PDCD4 proteins. Caspase-Glo3 assay was applied to assess caspase 3 activity. Dual-luciferase reporter assay was applied to verify the targeting relationship between TUG1 or PDCD4 and miR-181b-5p. Results Compared with the NG group, the cell activity decreased in the HG group, and LDH release, apoptosis rate, Bax, cleaved caspase 3 expression and caspase 3 activity increased (P<0.05), which could be antagonized by TUG1 knockdown or miR-181b-5p overexpression (P<0.05). Inhibition of miR-181b-5p was able to alleviate the impact of TUG1 silencing on cardiomyocyte viability and apoptosis under high glucose treatment (P<0.05). The overexpression of PDCD4 attenuated the promotion effect of miR-181b-5p up-regulation on the viability of cardiomyocytes treated with high glucose and the inhibitory effect on apoptosis. TUG1 was able to increase the expression of PDCD4 through adsorption of miR-181b-5p (P<0.05). Conclusion TUG1 promotes high glucose-induced cardiomyocyte apoptosis by down-regulating miR-181b-5p and up-regulating PDCD4.

Key words: taurine up-regulated gene 1, miR-181b-5p, programmed cell death protein 4, high glucose, cardiomyocytes, apoptosis

中图分类号:

R542.2

图/表 17

表1 qRT-PCR引物列表

Tab.1 Primers for qRT-PCR

基因名称	引物序列（5′→3′）	产物大小/bp
TUG1	上游：TCAGCCCACCGTAACAAT	184
	下游：CAAACTTCTCGGCGTCAT	184
PDCD4	上游：AAAACTCATCCCGGGACTCT	162
	下游：CTGCACCACCTTTCTTTGGT	162
miR-181b-5p	上游：CCAGCTGGGCTCACTGAACAATGA	202
miR-181b-5p	下游：CAACTGGTGTCGTGGAGTCGGC	202
GAPDH	上游：CCAGGTGGTCTCCTCTGA	197
	下游：GCTGTAGCCAAATCGTTGT	197
U6 snRNA	上游：GCTCGCTTCGGCAGCACA	128
	下游：GAGGTATTCGCACCAGAGGA	128

表2 各组AC16细胞活力、LDH释放及TUG1、miR-181b-5p表达比较

Tab.2 Comparison of viability, LDH release and expression of TUG1 and miR-181b-5p between two groups of AC16 cells

组别		细胞活力/%	LDH释放总量/%	TUG1	miR-181b- 5p
NG组		100.00±10.03	100.00±1.47	1.00±0.08	1.00±0.11
HG组	12 h	81.23±9.24^a	126.34±3.95^a	1.35±0.11^a	0.76±0.09^a
	24 h	60.45±5.16^a	176.51±5.87^a	2.16±0.18^a	0.51±0.06^a
	48 h	38.72±2.35^a	228.63±7.68^a	3.31±0.32^a	0.37±0.03^a
F		76.862^＊	697.645^＊	164.702^＊	75.206^＊

图1 流式细胞术检测各组AC16细胞凋亡

Fig.1 Flow cytometry detection of apoptosis in each group of AC16 cells

图2 Western blot检测各组AC16细胞中Bax、cleaved caspase 3蛋白表达 A：NG组；B：HG组；C：HG+sh-NC组；D：HG+sh-TUG1组；E：HG+miR-NC组；F：HG+miR-181b-5p组。

Fig.2 Western blot assay of Bax, cleaved caspase 3 protein expression in each group of AC16 cells

表3 各组AC16细胞TUG1和miR-181b-5p表达以及细胞活力、LDH释放比较

Tab.3 Comparison of TUG1 and miR-181b-5p expression as well as viability and LDH release between six groups of AC16 cells（n=6，$\bar{x}±s$）

组别	TUG1	miR-181b-5p	细胞活力/%	LDH释放总量/%
NG组	1.00±0.05	1.00±0.05	100.00±9.42	10.03±0.05
HG组	3.46±0.37^a	0.31±0.02^a	42.31±2.36^a	59.43±3.62^a
HG+sh-NC组	3.42±0.35	0.33±0.03	42.28±2.34	58.27±3.71
HG+sh-TUG1组	1.23±0.11^bc	0.74±0.04^bc	81.25±5.47^bc	22.34±1.52^bc
HG+miR-NC组	3.44±0.36	0.32±0.02	43.12±2.41	60.13±3.84
HG+miR-181b-5p组	1.38±0.14^bd	2.89±0.31^bd	80.42±4.65^bd	27.52±1.68^bd
F	128.928^＊	353.223^＊	148.302^＊	382.182^＊

表4 各组AC16细胞凋亡及相关蛋白表达比较

Tab.4 Comparison of apoptosis and related protein expression between six groups of AC16 cells （n=6，$\bar{x}±s$）

组别	凋亡率/%	Bax	cleaved caspase 3	caspase 3/（×10³ RLU）
NG组	3.48±0.11	1.00±0.02	1.00±0.03	21.43±1.14
HG组	26.59±2.31^a	2.51±0.17^a	2.38±0.21^a	68.54±4.69^a
HG+sh-NC组	26.72±2.35	2.48±0.19	2.45±0.26	69.72±5.11
HG+sh-TUG1组	14.12±1.06^bc	1.65±0.11^bc	1.32±0.09^bc	38.63±2.15^bc
HG+miR-NC组	25.89±2.42	2.45±0.23	2.29±0.31	66.49±6.04
HG+miR-181b-5p组	14.56±1.11^bd	1.52±0.08^bd	1.49±0.12^bd	32.41±0.17^bd
F	169.000^＊	106.345^＊	61.122^＊	161.682^＊

图3 通过starBase 3.0预测TUG1在miR-181b-5p中的结合位点

Fig.3 Prediction of TUG1 binding sites in miR-181b-5p by starBase 3.0

图4 流式细胞术检测各组AC16细胞凋亡

Fig.4 Flow cytometry results of apoptosis in each group of AC16 cells

图5 Western blot检测各组AC16细胞中Bax、cleaved caspase 3蛋白表达 A：HG+sh-TUG1组；B：HG+sh-TUG1+anti-miR-NC组；C：HG+sh-TUG1+anti-miR-181b-5p组。

Fig.5 Western blot assay of Bax, cleaved caspase 3 protein expression in each group of AC16 cells

表5 各组AC16细胞miR-181b-5p表达、活力、LDH释放和凋亡及其相关蛋白表达比较

Tab.5 Comparison of miR-181b-5p expression, viability, LDH release and apoptosis and its related protein expression between three groups of AC16 cells（n=6，$\bar{x}±s$）

组别	miR-181b-5p	细胞活力/%	LDH释放总量/%	凋亡率/%	Bax	cleaved caspase 3	caspase 3/ （×10³RLU）
HG+sh-TUG1组	0.75±0.06	81.27±5.45	23.41±1.54	14.18±1.05	1.68±0.15	1.35±0.11	38.54±2.12
HG+sh-TUG1+anti-miR-NC组	0.77±0.05	80.63±5.51	23.45±1.49	14.21±1.08	1.69±0.13	1.38±0.09	38.68±2.31
HG+sh-TUG1+anti-miR-181b-5p组	0.35±0.02^ab	53.45±2.36^ab	45.38±2.62^ab	23.96±1.21^ab	2.27±0.18^ab	2.14±0.23^ab	59.15±4.58^ab
F	155.446^＊	69.163^＊	252.339^＊	153.264^＊	28.604^＊	49.354^＊	82.171^＊

图6 通过starBase 3.0预测PDCD4在miR-181b-5p中的结合位点

Fig.6 Prediction of PDCD4 binding sites in miR-181b-5p by starBase 3.0

图7 Western blot检测各组AC16细胞中PDCD4蛋白表达 A：miR-NC组；B：miR-181b-5p组；C：anti-miR-NC组；D：anti-miR-181b-5p组。

Fig.7 Western blot assay of PDCD4 protein expression in each group of AC16 cells

表6 各组AC16细胞PDCD4表达、活力、LDH释放和凋亡及其相关蛋白表达比较

Tab.6 Comparison of PDCD4 expression, viability, LDH release and apoptosis and its related protein expression between three groups of AC16 cells

组别	PDCD4 mRNA	PDCD4蛋白	细胞活力/%	LDH释放总量/%	凋亡率/%	Bax	cleaved caspase 3	caspase 3/ （×10³RLU）
HG+miR-181b-5p组	1.00±0.08	0.98±0.06	80.35±4.63	27.56±1.72	14.53±1.08	1.54±0.11	1.51±0.13	32.45±2.03
HG+miR-181b-5p+pcDNA组	1.02±0.01	1.00±0.09	80.48±4.69	27.59±1.70	14.51±1.05	1.56±0.12	1.53±0.15	32.48±2.13
HG+miR-181b-5p+pc-PDCD4组	2.59±0.25^ab	2.13±0.18^ab	51.85±2.17^ab	43.78±2.54^ab	24.15±1.28^ab	2.19±0.15^ab	2.08±0.21^ab	61.23±4.64^ab
F	189.863^＊	176.857^＊	101.696^＊	128.099^＊	142.406^＊	50.192^＊	22.556^＊	164.458^＊

图8 流式细胞术检测各组AC16细胞凋亡

Fig.8 Flow cytometry results of apoptosis in each group of AC16 cells

图9 Western blot检测各组AC16细胞中PDCD4、Bax、cleaved caspase 3蛋白表达 A：HG+miR-181b-5p组；B：HG+miR-181b-5p+pcDNA组；C：HG+miR-181b-5p+pc-PDCD4组。

Fig.9 PDCD4, Bax and cleaved caspase 3 protein expression in each group of AC16 cells detected by Western blot assay

图10 Western blot检测各组AC16细胞中PDCD4蛋白表达 A：NG组；B：HG组；C：HG+sh-NC组；D：HG+sh-TUG1组；E：HG+sh-TUG1+anti-miR-NC组；F：HG+sh-TUG1+anti-miR-181b-5p组。

Fig.10 PDCD4 protein expression in each group of AC16 cells detected by Western blot assay

表7 各组PDCD4表达比较

Tab.7 Comparison of PDCD4 expression between six groups

组别	PDCD4 mRNA	PDCD4蛋白
NG组	1.00±0.02	1.00±0.03
HG组	2.48±0.31^a	1.76±0.22^a
HG+sh-NC组	2.51±0.35	1.78±0.24
HG+sh-TUG1组	1.29±0.21^bc	1.23±0.16^bc
HG+sh-TUG1+anti-miR-NC组	1.27±0.23	1.25±0.19
HG+sh-TUG1+anti-miR-181b-5p组	2.21±0.34^de	1.75±0.21^de
F	38.625^＊	19.805^＊

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LncRNA TUG1通过调节miR-181b-5p/PDCD4轴对高糖诱导的心肌细胞凋亡的影响

Impact of LncRNA TUG1 on high glucose-induced cardiomyocyte apoptosis by regulating the miR-181b-5p/PDCD4 axis

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