强直性脊柱炎患者外周血TLR4、JAK3基因表达与Th17/Treg失衡的关系

doi:10.11958/20240195

摘要/Abstract

摘要：

目的探究强直性脊柱炎患者外周血中Toll样受体4（TLR4）、Janus蛋白酪氨酸激酶3（JAK3）基因表达与辅助性T细胞17（Th17）/调节性T细胞（Treg）失衡的关系。方法 101例AS患者（AS组）按照AS疾病活动指数（BASDAI）评分分为AS稳定期（ASS）组（<4分）42例、AS活动期（ASA）组（≥4分）59例，另选同期于本院体检健康者为健康组50例。荧光定量PCR法检测外周血单个核细胞（PBMC）中TLR4、JAK3 mRNA表达，检测血清Th17、Treg细胞比例，并计算Th17/Treg比值。结果 AS组外周血PBMC中TLR4 mRNA、JAK3 mRNA表达高于健康组（P<0.05）。AS组外周血Th17比例及Th17/Treg高于健康组，Treg比例低于健康组（P<0.05）。较高水平TLR4 mRNA、JAK3 mRNA及Th17为AS的危险因素，较高水平Treg为AS保护因素。AS患者TLR4 mRNA、JAK3 mRNA分别与Th17比例及Th17/Treg呈正相关，与Treg比例呈负相关（P<0.05）。结论 AS患者PBMC中TLR4、JAK3基因呈高表达且Th17/Treg呈失衡状态，TLR4、JAK3基因高表达与AS患者Th17/Treg失衡密切相关。

关键词: 脊柱炎, 强直性, 单核细胞, Toll样受体4, Janus激酶3, Th17/Treg失衡

Abstract:

Objective To explore the relationship between the expression of Toll like receptor 4 (TLR4) and Janus protein tyrosine kinase 3 (JAK3) genes and the imbalance of helper T cell 17 (Th17)/regulatory T cells (Treg) in peripheral blood mononuclear cells (PBMCs) of patients with ankylosing spondylitis (AS). Methods A total of 101 AS patients were selected as the AS group. According to the AS Disease Activity Index (BASDAI) score, patients were divided into the AS stable phase (ASS) group (<4 points, 42 cases) and the AS active phase (ASA) group (≥ 4 points, 59 cases). Additionally, 50 healthy volunteers who underwent physical examinations at our hospital during the same period were selected as the control group (health group). Real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) was used to determine the mRNA expression of TLR4 and JAK3 in PBMCs, and the proportion of serum Th17 and Treg was measured and Th17/Treg levels were calculated. Results The levels of TLR4 mRNA and JAK3 mRNA in PBMCs of peripheral blood were higher in the AS group than those in the health group (P<0.05). The levels of Th17, proportion and Th17/Treg in peripheral blood were higher in the AS group than those of the health group (P<0.05). Higher levels of TLR4 mRNA, JAK3 mRNA, Th17, and Th17/Treg were risk factors for AS, while higher level of Treg was protective factors for AS. TLR4 mRNA and JAK3 mRNA in AS patients were positively correlated with Th17 ratio and Th17/Treg ratio, and negatively correlated with Treg ratio (P<0.05). Conclusion TLR4 and JAK3 genes are highly expressed and Th17/Treg is unbalanced in PBMC of AS patients. The high expression of TLR4 and JAK3 genes is closely related to Th17/Treg imbalance in AS patients.

Key words: spondylitis, ankylosing, monocytes, Toll-like receptor 4, Janus kinase 3, Th17/Treg imbalance

中图分类号:

R743.3

图/表 4

参考文献 20

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组别	n	年龄/岁	性别（男/女）	BMI/（kg/m²）
健康组	50	40.48±4.85	35/15	22.09±0.52
AS组	101	41.03±3.89	77/24	22.06±0.53
t或χ²		0.752	0.679	0.329

组别	n	年龄/岁	性别（男/女）	BMI/（kg/m²）
健康组	50	40.48±4.85	35/15	22.09±0.52
AS组	101	41.03±3.89	77/24	22.06±0.53
t或χ²		0.752	0.679	0.329

组别	n	TLR4 mRNA	JAK3 mRNA
健康组	50	1.57±0.53	1.34±0.51
AS组	101	2.69±0.71	1.99±0.51
t		9.869^＊＊	7.371^＊＊

组别	n	TLR4 mRNA	JAK3 mRNA
健康组	50	1.57±0.53	1.34±0.51
AS组	101	2.69±0.71	1.99±0.51
t		9.869^＊＊	7.371^＊＊

组别	n	Th17/%	Treg/%	Th17/Treg
健康组	50	0.61±0.06	8.90±1.05	0.07±0.01
AS组	101	1.15±0.15	4.60±0.47	0.25±0.04
t		24.472^＊＊	34.793^＊＊	31.291^＊＊