天津医药 ›› 2015, Vol. 43 ›› Issue (3): 249-251.doi: 10.11958/j.issn.0253-9896.2015.03.007

• 实验研究 • 上一篇    下一篇

Six1 在宫颈癌组织中的表达及意义

王彤 1, 朴龙镇 1, 崔雪莲 2, 杨洋 2, 南云泽 1△   

  1. 1 延边大学附属医院妇科(邮编 133000)2 延边大学医学院病理学教研室
  • 收稿日期:2014-10-21 修回日期:2014-12-30 出版日期:2015-03-15 发布日期:2015-03-15
  • 通讯作者: 南云泽 E-mail:yznan@ybu.edu.cn
  • 作者简介:王彤 (1987), 女, 医师, 硕士, 主要从事妇科肿瘤方面研究
  • 基金资助:
    国家自然科学基金(61371067); 吉林省科技厅中青年科研创新团队领军人才项目(20130521017JH)

Expression and significance of Six1 in cervical cancer

WANG Tong1, PIAO Longzhen1, CUI Xuelian2, YANG Yang2, NAN Yunze1△   

  1. 1 Department of Gynecology, Yanbian University Hospital, Yanji 133000, China; 2 Department of Pathology, Yanbian University Medical College
  • Received:2014-10-21 Revised:2014-12-30 Published:2015-03-15 Online:2015-03-15
  • Contact: NAN Yunze E-mail:yznan@ybu.edu.cn

摘要: 目的 探讨 Six1 基因在宫颈癌组织中的表达情况及其临床意义。方法 免疫组织化学 SP 法检测 123 宫颈癌组织, 49 例宫颈上皮内瘤变(CIN)组织及 32 例正常宫颈组织中 Six1 蛋白的表达。体外培养人宫颈癌 HeLa细胞, 免疫荧光染色观察 Six1 蛋白在细胞中的定位结果 宫颈癌组织 Six1 蛋白阳性表达率(72.3%)高于 CIN28.6%) 及正常宫颈组织 (15.6%), χ2分别为 13.11810.058 (均 P < 0.01)。不同肿瘤大小, 淋巴结及血道转移情况的宫颈癌组织中 Six1 蛋白阳性表达率差异有统计学意义(均 P < 0.01)。Six1 蛋白在 HeLa 细胞中主要定位于细胞浆和核仁。结论 Six1 与宫颈癌的发生发展密切相关, 可能成为宫颈癌的侵袭与转移的分子标志物。

关键词: 宫颈肿瘤; 基因, 同源盒; Six1; 免疫组织化学; 肿瘤浸润; 肿瘤转移; HeLa 细胞

Abstract: Objective To investigate the clinical significance of protein expression of Six1 in cervical cancer. Methods The immunohistochemical (IHC) staining was applied to detect the expression of Six1 protein in normal cervical tissues (n=32), cervical intraepithelial neoplasia (CIN) tissues (n=49) and cervical cancer tissues (n=123). The localization of Six1 protein was detected in vitro cultured HeLa cells using immunofluorescence (IF) staining. Results The positive rate of Six1was significantly higher in cervical cancer (72.3%) than that of CIN tissues (28.6%) and normal cervical tissues (15.6%, 2χ = 13.118 and 10.058 respectively, P < 0.01). There were significance differences in expression levels of Six1 protein between different tumor sizes and metastasis of cervical cancer (P < 0.01). The Six1 protein showed positive signals in cytoplasm and nucleoli in HeLa cells. Conclusion Six1 expression is associated with cervical cancer, which may be a potential
biomarker for invasion and metastasis of cervical cancer.


Key words: uterine cervical neoplasms, genes, homeobox, Six1, immunohistochemistry, neoplasm invasiveness, neoplasm metastasis, HeLa cells