天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (5): 461-464.doi: 10.11958/j.issn.0253-9896.2015.05.004

• 细胞与分子生物学 • 上一篇    下一篇

艾塞那肽对人舌鳞癌SCC-25细胞增殖、侵袭及凋亡的影响

黄超 1， 申菲菲 2， 李刚 3， 肇阅 4△   

  1. 1辽宁医学院附属第一医院口腔科 （邮编 121000）； 2辽宁医学院病理学教研室； 3辽宁医学院附属第一医院骨外科， 4高压氧治疗中心
  • 收稿日期:2014-10-21 修回日期:2014-12-29 出版日期:2015-05-15 发布日期:2015-05-25
  • 通讯作者: 肇阅 E-mail:zhaoyuezy123@126.com E-mail:zhaoyuezy123@126.com
  • 作者简介:黄超 （1982）， 硕士研究生， 主治医师， 主要从事口腔癌发病机制及治疗研究

Effects of exenatide on the cell proliferation, invasion and apoptosis of human tongue squamous cell carcinoma SCC-25

HUANG Chao1, SHEN Feifei2, LI Gang3, ZHAO Yue4△#br# #br#   

  1. 1 Department of Stomatology, First Affiliated Hospital of Liaoning Medical College, Jinzhou 121000, China; 2 Department of Pathology, Liaoning Medical University;3 Department of Orthopedics, 4 Hyperbaric Oxygen Therapy Center, First Affiliated Hospital of Liaoning Medical College
  • Received:2014-10-21 Revised:2014-12-29 Published:2015-05-15 Online:2015-05-25
  • Contact: ZHAO Yue E-mail:zhaoyuezy123@126.com E-mail:zhaoyuezy123@126.com

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摘要： 摘要： 目的 探讨艾塞那肽对人舌鳞癌 SCC-25 细胞增殖、 侵袭能力以及凋亡相关指标的影响。方法 体外培养 SCC-25 细胞， Western blot 检测 SCC-25 细胞中胰高血糖素样肽 1 受体（GLP-1R）表达。实验分 4 组： 对照组、 1、 10 和 100 nmol/L 艾塞那肽处理组。培养 24 h、 48 h 和 72 h 后 MTT 法检测各组细胞增殖能力， Transwell 实验检测各组细胞侵袭能力。Western blot 检测基质金属蛋白酶（MMP） -2、 Caspase-3 和 p38 丝裂原活化蛋白激酶（Phosphop38 MAPK）表达。结果 SCC-25 细胞表达 GLP-1R。与对照组相比， 艾塞那肽处理组细胞存活率及侵袭率明显降低（P < 0.05）， MMP-2 蛋白表达降低（P < 0.05）， Caspase-3 蛋白表达明显升高（P < 0.05）； 各指标变化呈现艾塞那肽浓度和时间依赖性。10 nmol/L 艾塞那肽处理组 24 h 后 Phospho-p38 MAPK 表达升高（P < 0.05）。结论 艾塞那肽可抑制 SCC-25 细胞增殖和侵袭， 且可能通过促进 Phospho-p38 MAPK 和 Caspase-3 的表达诱导细胞凋亡。

关键词: 艾塞那肽, 舌鳞状细胞癌, 胰高血糖素样肽1受体, 细胞增殖, 肿瘤侵袭, 凋亡

Abstract: Abstract: Objective To detect the effects of exenatide on the related indicators of proliferation, invasion and apopto⁃ sis of cell line SCC-25. Methods SCC-25 cells were cultured in vitro. The expression level of glucagon like peptide 1 re⁃ ceptor (GLP-1R) was determined by Western blot assay in SCC-25 cells. SCC-25 cells were divided into four groups: con⁃ trol group and exenatide group (1, 10 and 100 nmol/L). The ability of cell proliferation was detected using MTT assay after 24 h, 48 h and 72 h of culture. The ability of invasion was measured with Transwell assays. The expression levels of MMP- 2, Caspase-3 and Phospho-p38 MAPK were measured by Western blot assay. Results GLP-1 receptor expression was found in SCC-25 cells. Compared with control group, the cell survival rate, invasion rate and the expression of MMP-2 were signifi⁃ cantly decreased in SCC-25 group (P < 0.05). The expression of Caspase-3 were significantly increased (P < 0.05).Changes were in a concentration-dependent and time-dependent manner (P < 0.05). The expression of Phospho-p38 MAPK was sig⁃ nificantly increased at 24 h in 10 nmol/L exenatide group (P < 0.05). Conclusion Exenatide can inhibit the cell prolifera⁃ tion and invasion, which may contribute the apoptosis by promoting expressions of Phospho-p38 MAPK and Caspase-3 of SCC-25 cells.

Key words: exenatide, tongue squamous cell carcinoma, GLP-1R, cell proliferation, neoplasm invasiveness, apoptosis