天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (11): 1278-1281.doi: 10.11958/j.issn.0253-9896.2015.11.015

• 实验研究 • 上一篇    下一篇

薏苡茎醇提取物对荷 H22小鼠体内抗肿瘤作用

黄挺章 1， 李远辉 1， 郭圣奇 1， 卢善善 2， 齐梁煜 1， 冯雪萍 3， 黄锁义 4△#br#   

  1. 1广西百色市右江区， 右江民族医学院临床学院 （邮编533000）， 2护理学院， 3实验动物中心， 4药学院
  • 收稿日期:2015-02-27 修回日期:2015-07-16 出版日期:2015-11-15 发布日期:2015-11-15
  • 通讯作者: 黄锁义 E-mail: huangsuoyi@163.com E-mail:huangsuoyi@163.com
  • 作者简介:黄挺章(1988)， 男， 大学本科在读， 主要从事天然产物化学、 药物化学、 中药药理学等研究
  • 基金资助:
    国家自然科学基金资助项目 （81360684）； 广西自然科学基金资助项目 （2011GXNSFA018046）； 广西中医药科技专项资助课题 （GZKZ10128）； 广西教育厅课题 （201106LX425）； 广西中医药管理局课题 （gzzc1047）； 广西医学科学实验中心开放基金专项资助项目 （KFJJ2011-04）

The anti-tumor effect of Coix stalk alcohol extraction on H22 tumor-bearing mice

HUANG Tingzhang1, LI Yuanhui1, GUO Shengqi1, LU Shanshan2, QI Liangyu1, FENG Xueping3, HUANG Suoyi4△   

  1. 1 School of Clinical Medicine, 2 College of Nursing, 3 Experimental Animal Center, 4 School of Pharmacy, Youjiang Medical University for Nationalities, Baise, Guangxi 533000, China
  • Received:2015-02-27 Revised:2015-07-16 Published:2015-11-15 Online:2015-11-15
  • Contact: HUANG Suoyi E-mail: huangsuoyi@163.com E-mail:huangsuoyi@163.com

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摘要： 目的 研究薏苡茎醇提取物对荷 H22小鼠体内抗肿瘤作用。方法 采用小鼠肝癌 H22腹水型瘤细胞建立荷瘤小鼠动物模型， 将 84 只模型小鼠随机均分为薏苡茎醇提取物剂量 1~5 组、 模型对照组、 环磷酰胺组， 分别灌胃薏苡茎醇提取液 10、 8、 6、 4、 2 g/kg， 等体积生理盐水和环磷酰胺 0.02 g/kg， 每日 1 次， 连续 8 d。观察小鼠的活动能力、 腹部膨隆大小及出现的时间、 毛发和摄食、 饮水量变化等情况。测量荷 H22小鼠的实体瘤质量， 计算抑瘤率， 并计算肝脏指数、 脾脏指数和胸腺指数。结果 模型对照组最先出现腋下肿瘤鼓起且生长最快， 自主活动减少、 食欲下降、 毛色开始暗淡等反应， 剂量 1、 2 组次之， 剂量 5 组和环磷酰胺组最慢。薏苡茎醇提取物剂量 1~5 组瘤质量[分别为 （0.47±0.18）、（0.37±0.13）、（0.34±0.10）、（0.30±0.11）、（0.28±0.09） mg]均低于模型对照组(0.60±0.21)mg[F=5.700， P＜0.05]， 薏苡茎醇提取物剂量 1~5 组、 环磷酰胺组抑瘤率依次升高 （分别是 21.67%、 38.33%、 43.33%、50.00%、 53.33%和60.00%）。环磷酰胺组和薏苡茎醇提取物组的肝脏指数、 脾脏指数和胸腺指数均低于模型对照组（剂量 1 组脾脏指数除外）； 薏苡茎醇提取物各剂量组的肝脏指数低于环磷酰胺组， 脾脏指数、 胸腺指数与环磷酰胺组差异无统计学意义。结论 薏苡茎醇提取物对荷 H22小鼠体内肿瘤和肝脏损害有抑制作用。

关键词: 抗肿瘤药(中药), 薏苡, 薏苡茎, 醇提取物, H22, 体内抗肿瘤, 环磷酰胺

Abstract: Objective To study the anti-tumor effects of alcohol extraction of Coix stalk objects on H22 tumor-bearing mice. Methods The animal model of tumor bearing mice with H22 ascitic tumor cells was established. Eighty-four model mice were randomly and equally divided into Coix stalk extract groups 1-5 (10, 8, 6, 4 and 2 g/kg), model control group and cyclophosphamide group. Mice were treated orally with Coix stalk alcohol extraction solution (10, 8, 6, 4 and 2 g/kg), cyclophosphamide 0.02 g/kg
and normal saline once a day for 8 days for Coix stalk extract group, cyclophosphamide group and model control group. The mouse activity, the size and the appearance of time of abdominal swelling, and changes of hair, feeding and drinking water quantity were observed in groups of mice. The solid tumor mass was measured in H22 tumor-bearing mice. The tumor inhibitory rate, liver index, spleen index and thymus index were calculated. Results The axillary tumor muster was found first in model control group with the fastest growth, reduced independent activity, decreased appetite and dim in hair color, followed by the Coix stalk extract group 1 and group 2. The last was Coix stalk extract group 5 and cyclophosphamide group. The solid tumor mass were (0.47±0.18), (0.37±0.13), (0.34±0.10), (0.30±0.11) and (0.28±0.09) mg for Coix stalk alcohol extract groups 1-5, which were significantly lower than those of model control group (0.60 mg±0.21 mg, F=5.700， P＜0.05). The tumor inhibition rates were 21.67%, 38.33%, 43.33%, 50.00%, 53.33% and 60.00% in Coix stalk extract groups 1-5 and cyclophosphamide group. The liver index, spleen index and thymus index were lower in cyclophosphamide group and Coix stalk alcohol extract groups than those of model control group (except for the spleen index of Coix stalk extract group 1). The liver index was lower in Coix stalk ethanol extract groups than that of cyclophosphamide group. There were no significant differences in the spleen index, thymus index between Coix stalk ethanol extract groups and cyclophosphamide group. Conclusion Coix stalk alcohol extract has inhibitory effects on the tumor and liver damage in H22 mice.

Key words: Antineoplastic Drugs (TCD, COIX LACRYMA-JOBI L, Coix stalk, alcohol extract, H22, anti tumor in vivo,  cyclophosphamide