天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (11): 1311-1314.doi: 10.11958/j.issn.0253-9896.2015.11.024

• 临床研究 • 上一篇    下一篇

口腔鳞状细胞癌中 FEZ1 和 HIF-1α蛋白表达及预后研究

张晋弘 1， 刘俊英 2， 刘健 3， 刘月平 2△#br#   

  1. 1河北医科大学第一医院口腔科 （邮编 050031）； 2河北医科大学第四医院病理科； 3河北医科大学第四医院口腔科
  • 收稿日期:2015-04-07 修回日期:2015-06-22 出版日期:2015-11-15 发布日期:2015-11-15
  • 通讯作者: 刘月平 E-mail： annama@163.com E-mail:zjh888fff@126.com
  • 作者简介:张晋弘 （1975）， 男， 硕士， 主治医师， 主要从事口腔颌面外科研究
  • 基金资助:
    河北省卫生厅科研基金项目 （项目编号 20100281）

Expressions of FEZ1 and HIF-1α protein in oral squamous cell carcinoma and its prognosis

ZHANG Jinhong1， LIU Junying2， LIU Jian3， LIU Yueping2△#br#   

  1. 1 Department of Stomatology, the First Affiliated Hospital, Hebei Medical University, Shijiazhuang 050031, China; 2 Department of Pathology, the Fourth Affiliated Hospital, Hebei Medical University; 3 Department of Stomatology, the Fourth Affiliated Hospital, Hebei Medical University
  • Received:2015-04-07 Revised:2015-06-22 Published:2015-11-15 Online:2015-11-15
  • Contact: LIU Yueping E-mail： annama@163.com E-mail:zjh888fff@126.com

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摘要： 目的 探讨抑癌基因 FEZ1 和缺氧诱导因子 （HIF） -1α在口腔鳞状细胞癌 （OSCC） 中的表达及其与临床病理特征及预后的关系。方法 选取 OSCC 组织标本 146 例， 正常黏膜组织标本 80 例， 同时选取新鲜 OSCC 组织、 癌旁组织和正常黏膜组织各 30 例。免疫组化法检测 146 例组织标本和 80 例正常黏膜组织中 FEZ1 和 HIF-1α表达，分析二者表达与患者临床病理特征及预后的关系。 Western blot 检测 30 例新鲜 OSCC 组织及其对应癌旁组织、 正常黏膜组织中 FEZ1 和 HIF-1α 表达情况。结果 146 例 OSCC 组织 FEZ1 蛋白阳性表达率 （30.14%） 低于正常口腔黏膜组织（81.25%）， HIF-1α蛋白阳性表达率（71.23%）高于正常黏膜组织（12.50%， χ2分别为 54.076 和 71.317， 均 P <0.01）。146 例 OSCC 中 FEZ1 的表达水平与临床分期、 肿瘤直径、 分化程度有关， HIF-1α的表达水平与性别、 淋巴结转移、 肿瘤直径、 分化程度有关。FEZ1α蛋白阳性表达与阴性表达患者 5 年生存率差异无统计学意义， 而 HIF-1α蛋白阳性表达者 5 年生存率低于阴性表达患者 （P < 0.05）。30 例正常口腔黏膜组织、 癌旁组织及 OSCC 组织中， FEZ1表达水平依次降低（P < 0.01）， HIF-1α表达水平依次升高（P < 0.01）。结论 FEZ1 和 HIF-1α 可能参与了 OSCC 的发生、 发展， 检测 FEZ1 和HIF-1α表达可作为 OSCC 生物学行为的参考指标之一。
 

关键词: 口腔鳞状细胞癌, FEZ1 , 缺氧诱导因子-1α, 免疫组织化学, 蛋白免疫印迹, 临床特征, 生存分析

Abstract: Objective To investigate the expressions of tumor suppressor gene FEZ1 and hypoxia inducible factor (HIF)- 1α in oral squamous cell carcinoma (OSCC), and their correlation with clinicopathological features and prognosis. Methods A total of 146 specimens of OSCC and 80 normal oral mucosa were used to detect the expressions of FEZ1 and HIF-1α by immunohistochemistry. Thirty specimens of fresh OSCC tissues, 30 samples of the paracancerous tissues and 30 normal mucosa tissues were also included in the study. The relationship between the expressions of FEZ1 and HIF-1α, the clinicopathological features and the prognosis of the patients were analyzed. Western blot assay was used to detect the expressions of FEZ1 and HIF-1α in 30 fresh OSCC tissues, 30 samples of the paracancerous tissues and 30 normal mucosa tissues. Results In OSCC tissues, the positive rate of FEZ1 protein (30.14%) was significantly lower than that in normal oral mucosa (81.25%), and the positive rate of HIF-1α protein (71.23%) was significantly higher than that in normal mucosa (12.50%，χ2=54.076 and 71.317, P < 0.01). There was a correlation between the expression of FEZ1 and clinical stage, tumor size and differentiation. The expression of HIF-1α was correlated with gender, lymph node metastasis, tumor size and differentiation. There was no significant difference in 5-year survival rate between positive and negative FEZ1 expression groups. The 5-year survival rate was significantly lower in HIF- 1α positive expression group than that of HIF- 1α negative expressiongroup (P < 0.05). The expression level of FEZ1 was decreased by the sequences in normal oral mucosa, paracancerous tissue and OSCC (P < 0.01), but the expression level of HIF-1α was increased in turn (P < 0.01). Conclusion FEZ1 and HIF-1α may be involved in the occurrence and progress of OSCC. The expressions of FEZ1 and HIF-1α can be used as a reference for the biological behavior of OSCC.

Key words: oral squamous cell carcinoma, FEZ1, hypoxia inducible factor-1α, immunohistochemistry, Western blot;
clinical features, survival analysis