天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (1): 51-53.doi: 10.3969/j.issn.0253-9896.2015.01.013

• 实验研究 • 上一篇    下一篇

苦参素对内毒素性休克致大鼠肾功能损伤的保护作用研究

梅志勤 1， 董海彦 2， 冯军花 3， 郭云 1， 刘志权 1， 王春岚 4△#br#   

  1. 1 石家庄市第五医院检验科（邮编 050021）； 2河北省石家庄市裕华区人口计划生育技术站； 3 石家庄， 河北医科大学第四医院检验科；4石家庄市第五医院感染控制科
  • 收稿日期:2014-01-24 修回日期:2014-07-23 出版日期:2015-01-15 发布日期:2015-01-30
  • 通讯作者: 王春岚E-mail： 13832353049@163.com E-mail:yjfnsc19@1633.com
  • 作者简介:梅志勤（1968）， 女， 主管检验师， 主要从事危重病的检验分析

Protective effects of oxymatrine injection on renal function of rats with endotoxic shock

MEI Zhiqin1, DONG Haiyan2, FENG Junhua3, GUO Yun1, LIU Zhiquan1, WANG Chunlan4△   

  1. 1 Department of Clinical Laboratory, The Fifth Hospital of Shijiazhuang, Shijiazhuang 050021， China; 2 Family Planning Technological Station, Yuhua District of Shijiazhuang ; 3 Department of Clinical Laboratory, The Fourth Hospital of Hebei Medical University; 4 Infection Control Department, The Fourth Hospital of Shijiazhuang
  • Received:2014-01-24 Revised:2014-07-23 Published:2015-01-15 Online:2015-01-30
  • Contact: E-mail： 13832353049@163.com E-mail:yjfnsc19@1633.com

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摘要： 目的 观察苦参素对内毒素性休克大鼠肾损伤的保护作用。 方法 将 24 只雄性 Wistar 大鼠随机分为正常组（8 只）、内毒素性休克组（8 只）和苦参素治疗组（8 只）， 内毒素性休克组和苦参素治疗组从股静脉注射脂多糖（LPS， 15 mg/kg）建立内毒素性休克模型， 造模 15 min 后， 苦参素治疗组自股静脉输注苦参素注射液， 观察其对平均动脉压（MAP）影响， 实验结束后取材测定血浆中反映肾功能指标的尿素（Urea）、肌酐（Cr）含量， 及肾组织匀浆中炎症因子白介素（IL）-6、IL-10、肿瘤坏死因子-α（TNF-α）的含量。 结果 苦参素治疗可防止内毒素性休克大鼠 MAP 的进行性下降； 内毒素性休克组大鼠血浆 Urea、Cr 含量显著高于正常组， 肾组织内 IL-6、IL-10 和 TNF-α明显上升， 苦参素治疗组大鼠血浆 Urea、Cr 含量显著低于内毒素性休克组， 肾组织内 IL-6 和 TNF-α显著下降。 结论 苦参素可对内毒素性休克后的肾功能提供一定的保护作用， 其机制可能与抑制肾内促炎性细胞因子释放有关。

关键词: 苦参；苦参碱；休克, 脓毒性；肾；苦参素；内毒素性休克；肾损伤

Abstract: Abstract： Objective To evaluate the protective effects of oxymatrine injection on rats with endotoxic shock. Methods Wistar rat model of endotoxic shock was produced in this study. Twenty-four Wistar rats were randomly divided into normal control group (n=8), endotoxic shock group (n=8) and oxymatrine injection treatment group (n=8). Fifteen min⁃ utes after the infusion of LPS (15 mg/kg) from femoral vein, oxymatrine was injected from femoral vein in treatment group, then we observed the mean arterial pressure (MAP) for six hours. At the end of experiment blood samples were harvested for measurement of urea and creatinine (Cr), which reflect renal function. Also contents of IL-6, IL-10, TNF-α in the renal ho⁃ mogenate were detected. Results Oxymatrine can prevent progressive decrease of MAP in endotoxin shock treatment group. The contents of plasma urea and Cr were significantly higher in endotoxin shock group than those of control group. The contents of IL-6, IL-10 and TNF-α in renal homogenate increased obviously, but after the injection of oxymatrine, the contents of urea and Cr significantly decreased in treatment group, also IL- 6 and TNF- α were significantly declined. Conclusion Oxymatrine provides protection at renal function after endotoxin shock, and its mechanism may be related to inhibit the releasing of inflammatory cytokines in kidney.

Key words: sophora flavescens, matrine, shock, septic, kidney, oxymatrine, endotoxic shock, injury of renal func? tion