天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (1): 57-60.doi: 10.3969/j.issn.0253-9896.2015.01.015

• 临床研究 • 上一篇    下一篇

TNF-α和 HSP70-2 基因多态性与急性胰腺炎的相关性研究

胡家平， 庄建新△， 李勇， 余永欢， 胡庆红， 赖银英， 吴安涛#br#   

  1. 南昌大学第一附属医院普外科五病区（邮编 330006）
  • 收稿日期:2014-07-03 修回日期:2014-09-05 出版日期:2015-01-15 发布日期:2015-01-30
  • 通讯作者: 庄建新E-mail： zjx136@qq.com E-mail:2030047577@qq.com
  • 作者简介:胡家平（1967）， 男， 副主任医师， 副教授， 硕士生导师， 硕士， 主要从事胃肠肿瘤研究
  • 基金资助:
    江西省科技厅支撑计划

Polymorphisms of TNF-α gene and HSP70-2 gene in patients with acute pancreatitis

HU Jiaping, ZHUANG Jianxin△, LI Yong, YU Yonghuan, HU Qinghong, LAI Yinying, WU Antao#br# #br#   

  1. The First Affiliated Hospital of Nanchang University, Nanchang 330006, China
  • Received:2014-07-03 Revised:2014-09-05 Published:2015-01-15 Online:2015-01-30
  • Contact: E-mail： zjx136@qq.com E-mail:2030047577@qq.com

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摘要： 　　　摘要:目的 探讨肿瘤坏死因子（TNF）-α和热休克蛋白（HSP）70-2 基因多态性与急性胰腺炎（AP）的相关性。方法 运用聚合酶链式反应-限制性片段长度多态性（PCR-RLFP）检测 72 例 AP 患者(AP 组)和 71 例正常人（对照组）的 TNF-α及 HSP70-2 基因多态性。 结果 AP 组与对照组的 TNF-α基因型和等位基因比例间差异无统计学意义； AP 组中， 重型胰腺炎（SAP）和轻型胰腺炎（MAP）的 TNF-α基因型和等位基因间差异无统计学意义。 TNF-α和 HSP70-2 基因 AA 型与 GA+GG 型患者的白细胞、C 反应蛋白（CRP）、淀粉酶、三酰甘油、血糖差异均无统计学意义； AP 组的 HSP70-2 基因 GA+GG 型患者比例高于对照组（69.4% vs 49.3%）， AP 组携带的 G 等位基因比例高于对照组（46.5% vs 31.7%）,差异均有统计学意义。 AP 组中， SAP 的 GA+GG 型比例高于 MAP（81.0% vs 53.3%）， 差异有统计学意义； 而 G 等位基因在 SAP 和 MAP 间无明显差异（P＞ 0.05）。 结论 TNF-α基因多态性与 AP 不相关， HSP70-2 基因多态性与 AP 相关， 携带 G 等位基因发生重症 AP 的可能性增大， 可能是重症 AP 的易感因素之一。

关键词: 肿瘤坏死因子α； HSP70 热休克蛋白质类； 胰腺炎； 基因多态性； HSP70-2； 多态性, 限制性片断长度； 聚合酶链反应

Abstract: Abstract： Objective To investigate the association of tumor necrosis factor (TNF)-α, heat shock protein (HSP)70-2 gene polymorphisms and susceptibility of acute pancreatitis(AP). Methods Using case-control method， The gene polymor⁃ phism of TNF- α and HSP70- 2 was detected by PCR- RLFP in 72 patients with AP and 71 healthy controls. Results There were no significant differences in proportion of TNF-α genotype and alleles between AP and control groups (P > 0.05). There were no significant differences in TNF-α genotype and alleles between severe acute pancreatitis (SAP) and light acute pancreatitis (MAP) of AP group (P > 0.05). There were no significant differences in white blood cell count, C-reactive pro⁃ tein (CRP), amylase, three acyl glycerin and glucose between TNF-a and HSP70-2 gene of AA type and GA+GG type pa⁃ tients (P >0.05). The HSP70-2 genotype GA+GG proportion was significantly higher in AP group than that of control group （69.4% vs 49.3%). The ratio of patients with G allele was significantly higher in AP group than that of control group（46.5% vs 31.7%). The ratio of patients with GA + GG type AP was significantly higher in SAP patients than that of MAP patients of AP group（81.0% vs 53.3%). There was no significant difference in G allele between SAP and MAP patients (P＞ 0.05). Conclusion TNF-α polymorphisms is not associated with acute pancreatitis. There is an association between HSP70-2 polymorphisms and acute pancreatitis. Carrying the G allele increases the possibility of a severe acute pancreatitis， which is one of the genetic susceptibility factors of severe acute pancreatitis.　　　

Key words: tumor necrosis factor-alpha； HSP70 heat-shock proteins； pancreatitis； genetic polymorphism； HSP70-2； polymorphism,restriction fragment length, polymerase chain reaction