天津医药

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PKC通路激活在七氟烷后处理减轻大鼠心肌缺血再灌注损伤中的作用

李波,吕国义,邓廼封   

  1. 天津医科大学第二医院
  • 收稿日期:2010-01-11 修回日期:2010-02-01 出版日期:2010-10-15 发布日期:2010-10-15
  • 通讯作者: 李波

The role of protein kinase-C pathway in protective effect of sevoflurane post-conditioning on rat’s myocardial ischemia/reperfusion injury in Vivo

  • Received:2010-01-11 Revised:2010-02-01 Published:2010-10-15 Online:2010-10-15

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摘要： 摘要 目的：评价蛋白激酶C（PKC）信号转导通路的激活在1MAC七氟烷后处理减轻在体大鼠心肌缺血/再灌注损伤的意义。方法：健康SD大鼠40只随机分为假手术组（S）、1MAC七氟烷后处理组（Sevo）、1MAC七氟烷后处理组+白屈菜红碱（Chelerythrine）（Sevo+C）、缺血再灌注损伤组（I/R），每组10只。再灌注末测定各组大鼠血清肌酸激酶同工酶（CK-MB-MB）活性；实验结束，处死动物，对心肌组织行HE染色法、TTC染色，测定心肌髓过氧化物酶（MPO）活性、心肌组织Ca2+ 含量、超氧化物歧化酶（SOD）含量。结果：与S组比较，Sevo组 、Sevo+C组和I/R组再灌注末血清CK-MB活性、心肌组织中MPO活性、Ca2+ 含量均明显升高，有统计学意义(P<0.05）。Sevo组 、Sevo+C组与I/R组相比较，再灌注末血清CK-MB活性、心肌组织中MPO活性、Ca2+ 含量均明显降低，有统计学意义(P<0.05）。与S组比较，Sevo组 、Sevo+C组再灌注末SOD活性升高(P<0.05），I/R组活性降低(P<0.05）。HE染色表明：Sevo组、Sevo+C组与I/R组心肌细胞均出现了不同程度的损伤。TTC染色表明：Sham组心肌无梗死，Sevo组和Sevo+C组与I/R组比较，心肌梗死面积显著减小（P<0.05），Sevo组与Sevo+C组心肌梗死面积比较显著减小（P<0.01）。结论：1MAC七氟烷后处理可通过激活PKC通路，减少细胞内钙超载、清除损伤性氧自由基减轻心肌缺血再灌注后的损伤。

关键词: 七氟烷后处理, 缺血/再灌注损伤, PKC

Abstract: Abstract Objectives ：To evaluate the factor of signal pathway-protein kinase-C(pkc) in protective effect of one MAC sevoflurane post-conditioning on rat’s myocardial ischemia/reperfusion injury in Vivo. Methods：Rats model of Myocardial ischemia-reperfusion Injury were randomly divided into 4 groups：groupⅠsham operation (S); groupⅡischemia/reperfusion injury (I/R); groupⅢone MAC sevoflurane post-conditioning (Sevo); groupⅣSevo +Chelerythrine（Sevo+C）. The activity of CK-MB、SOD、MPO and concentration of Calcium ion were measured at the end of reperfusion. Myocardial infarction size was determined by dyeing with hematoxylin eosin (HE) and triphenyltetrazolium staining at the end of reperfusion. Results ：As far as the activity of CK-MB、SOD、MPO and concentration of Calcium ion, there are great significant differences among four groups at the end of reperfusion（P<0.05）. The concentration of Calcium ion in groupⅡ,groupⅢand groupⅣ are higher than groupⅠ（P<0.05）. Compared with groupⅡ, infarction size in groupⅢ、 groupⅣreduce significantly（P<0.05）. There is also great significant difference of infarction size between groupⅢand groupⅣ（P<0.01）.Conclusion: One MAC sevoflurane post-conditioning can attenuate ischemia/reperfusion injury through the activation of PKC signal pathway.

Key words: Sevoflurane postcondtioning, ischemia/reperfusion injury, PKC