天津医药

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胃肠道肿瘤细胞E2F-1转录因子活性对细胞周期的影响

林彬1,周肖英2,程剑2   

  1. 1. 江苏无锡卫生高等职业技术学校
    2. 无锡卫生高等职业技术学校
  • 收稿日期:2010-01-07 修回日期:2010-04-17 出版日期:2010-10-15 发布日期:2010-10-15
  • 通讯作者: 林彬

The activity of E2F-1 transcriptional factor in gastrointestinal cancer cell lines and its effect on cell cycle

  • Received:2010-01-07 Revised:2010-04-17 Published:2010-10-15 Online:2010-10-15
  • Contact: BinLin

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摘要： 摘要：目的 探讨癌细胞E2F-1因子的表达状态和细胞周期的关系。方法 采用免疫荧光组织化学染色法，对8株胃肠道肿瘤细胞系进行了E2F-1转录因子的表达进行研究，并采用流式细胞术检测肿瘤细胞周期的变化。结果 依据E2F-1阳性表达的程度不同，将8株肿瘤细胞系分为E2F-1高表达组（包括HGC-27、BGC-823、SGC-7901、HT29）和E2F-1低表达组（包括MKN45、MKN28、SH288、SW-620）。E2F-1高表达组G1/G0期、S期、细胞凋亡比例分别为33.48±4.61%、63.91±5.02%、1.50±0.56%，低表达组G1/G0期、S期、细胞凋亡比例为58.58±3.41%、35.38±4.03%、5.61±1.44%。两组间有明显差别。结论 肿瘤细胞E2F-1因子的表达状态，对细胞周期有明显的影响。E2F-1阳性的肿瘤细胞则倾向于更高的增殖速率。

关键词: 胃肠道肿瘤, 转录因子, 细胞周期, 免疫组化, 流式细胞术

Abstract: Abstract：Objective To investigate the activity of E2F-1 transcriptional factor in gastrointestinal cancer cells and its effect on cell cycle. Methods The expression of E2F-1 factor in 8 gastrointestinal cancer cell lines was detected immunohistochemically, and the cell cycle of cancer cells was analyzed by flow cytometry. Results The gastrointestinal cancer cells were divided into two groups, the E2F-1 high-expression group including HGC-27, BGC-823, SGC-7901, HT29, the E2F-1 low-expression group including MKN45, MKN28, SH288, SW-620. The ratios of G1/G0 phase, S phase and apoptosis in high-expression group were 33.48±4.61%, 63.91±5.02%, 1.50±0.56%, and in low-expression group were 58.58±3.41%, 35.38±4.03%, 5.61±1.44%. There were differences between these two groups. Conclusion The status of E2F-1 expression in cancer cells has a great effect on cell cycle. The E2F-1-positive cancer cells have a potency to grow with high rate.

Key words: Gastrointestinal cancer, Transcriptional factor, Cell cycle, Immunohistochemistry, Flow cytometry