天津医药

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肠梗阻对肠上皮Claudin1 mRNA表达的影响

孙刚,于向阳,周振理   

  1. 天津市南开医院
  • 收稿日期:2010-07-20 修回日期:2010-07-31 出版日期:2010-10-15 发布日期:2010-10-15
  • 通讯作者: 于向阳

The changes of intestinal epithelial Claudin1 mRNA expression in the occurrence after intestinal obstruction

  • Received:2010-07-20 Revised:2010-07-31 Published:2010-10-15 Online:2010-10-15

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摘要： 目的：了解急性肠梗阻发生后肠道上皮细胞间紧密连接破坏的时相性特点。方法：选用健康大耳白兔，随机分为正常对照组（Norm）、假手术组（Sham）、梗阻1h组（H1）、梗阻6h组（H6）、梗阻12h组（H12）、梗阻24h组（H24）、梗阻48h组（H48）、梗阻72h组（H72），每组6只。利用实时荧光定量RT-PCR技术，对动物末段小肠上皮的紧密连接相关蛋白Claudin1的mRNA表达情况进行相对定量分析。结果：梗阻发生后6h已经发现Claudin1 mRNA表达已经显著减少至正常表达量的43%（p<0.05），12h为19%（p<0.01），24h为10%，48h-72h阶段表达量很低。结论：肠梗阻发生后早期（6h）已经发生机械屏障的破坏，表现为紧密连接蛋白上游基因的低表达，随着梗阻进展，这种低表达更加显著，至梗阻48h-72h阶段，这一基因的表达水平已经很低。提示这一阶段肠上皮的完整性已经完全失去，肠道机械屏障破坏严重。 关键词：肠道机械屏障，紧密连接，Claudin，实时荧光定量逆转录聚合酶链反应

关键词: 肠道机械屏障, 紧密连接, Claudin, 实时荧光定量逆转录聚合酶链反应

Abstract: Objective: To understand the characteristics of phase of destruction of tight junctions in intestinal epithelial cells after the occurrence of acute intestinal obstruction. Methods: Healthy rabbits were randomly divided into normal control group (Norm), sham-operated group (Sham), obstruction 1h group (H1), obstruction 6h group (H6), obstruction 12h group (H12), obstruction 24h group (H24), obstruction 48h group (H48), obstruction 72h group (H72), n = 6. the relative quantitative analysis of the animals of the last paragraph of the intestinal epithelial tight junction-associated protein Claudin1’s mRNA’s expression were made through the real-time fluorescence quantitative RT-PCR technology. Results: The experiments showed thatClaudin1 mRNA expression has been found to have significantly reduced levels to normal about 43% (p <0.05), 12h to 18% (p <0.01), 24h to 11% after 6h obstruction , 48h-72h phase expression was very low. Conclusions: mechanical barriers have been destroyed at Early stage after the occurrence of intestinal obstruction (6h), the manifestation was lowly expressed genes for the tight junction protein upstream gene expression, with the obstruction of progressing, the low expression was more prominent at 48h-72h phase of obstruction. Indicated that this stage of intestinal epithelial integrity is completely lost, seriously destruction of intestinal mechanical barrier . Key words: gastro-intestinal mechanical barriers, tight junctions, Claudin, real-time fluorescence quantitative reverse transcription polymerase chain reaction

Key words: gastro-intestinal mechanical barriers, tight junctions, Claudin, real-time fluorescence quantitative reverse transcription polymerase chain reaction