天津医药

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乳腺癌组织中TRβ1基因表达及临床意义

令亚琴1,许小英2,张茜3,令晓玲4,汪泳5   

  1. 1. 兰州大学基础医学院病理生理学研究所
    2. 兰州大学附属医院检验科
    3. 兰州军区兰州总医院
    4. 兰州大学附属医院肿瘤内科
    5. l兰州军区兰州总医院消化内科
  • 收稿日期:2010-07-01 修回日期:2010-09-16 出版日期:2010-12-15 发布日期:2010-12-15
  • 通讯作者: 汪泳

Expression of TRβ1 gene mRNA in breast cancer and its clinical significance

  • Received:2010-07-01 Revised:2010-09-16 Published:2010-12-15 Online:2010-12-15
  • Contact: Yong WANG

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摘要：  目的:探讨甲状腺素受体β1(TRβ1)在乳腺癌组织中的表达及临床意义。方法:运用半定量逆转录聚合酶链反应(RT-PCR)技术检测40例乳腺癌及10例正常乳腺组织中TRβ1基因表达情况。结果:TRβ1基因在10例正常乳腺组织中全部表达,而TRβ1基因在40例乳腺癌组织中mRNA表达下降34例(85%)。乳腺癌组织中TRβ1基因表达明显低于正常乳腺组织(P<0.05)。不同年龄的患者TRβ1基因mRNA的表达差异无统计学意义(P>0.05),在不同临床分期、淋巴有无结转移、ER的阳性与阴性表达及肿瘤大小不同的患者中,TRβ1基因mRNA的表达差异有统计学意义(P<0.05)。结论:TRβ1在乳腺癌的浸润和转移方面起重要作用,可成为评估乳腺癌严重程度的新指标。 

关键词: 乳腺癌, 甲状腺激素受体β1, mRNA, RT-PCR

Abstract: Abstract Objective:. To study the expressions of TRβ1 mRNA and clinical significance in breast cancer.Methods：The expressions of TRβ1mRNA in 40 cases of breast cancer and 10 cases of normal tissue were detected and analysed by using reverse-transcription PCR (RT-PCR). Results：The TRβ1 gene was expressed in all normal breast tissue. By contrast, reduced expression of TRβ1was found in 34 of the 40 breast cancer tissue samples (85%). The expression levels of TRβ1 were significantly lower in cancer group than those of normal group (P < 0.05).The expressions of TRβ1mRNA were significantly related with the depth of invasion, lymph-node metastasis and TNM stage(P < 0.05). but not related with age (P ＞ 0.05)，. Conclusion： TRβ1 play an important role in invasion and metastasis of the breast cancer. Detection of TRβ1 may be a new target for cancer diagnosis to assess the severity of breast cancer.

Key words: breast cancer, TRβ1gene, mRNA, RT-PCR