天津医药

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胰岛素对RF/6A细胞VEGF受体表达的影响

周赛君1,于珮1,于徳民1,陈松2   

  1. 1. 天津医科大学代谢病医院
    2. 天津市眼科医院
  • 收稿日期:2010-03-25 修回日期:2010-07-07 出版日期:2010-12-15 发布日期:2010-12-15
  • 通讯作者: 周赛君

The effects of insulin on the expression of VEGFR in RF/6A cells

  • Received:2010-03-25 Revised:2010-07-07 Published:2010-12-15 Online:2010-12-15

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摘要： 目的:探讨胰岛素对视网膜血管内皮细胞RF/6A血管内皮细胞生长因子受体(VEGFR)的表达及血管新生的影响。方法:分别用0、1和100nmol/L的人胰岛素处理RF/6A细胞,采用MTS实验、细胞迁移实验、管腔形成实验分别检测胰岛素对RF/6A细胞增殖、迁移、管腔形成等生物学功能,RT-PCR、Western blot技术检测VEGFR的表达、磷酸化活性。结果:胰岛素可促进RF/6A的增殖、迁移、管腔形成(P<0.05或P<0.01),促进VEGFR2 mRNA的表达和VEGFR2蛋白的磷酸化(P<0.05或P<0.01),而对VEGFR1 mRNA表达的影响差异无统计学意义(P>0.05)。结论:胰岛素可经VEGFR2信号促进RF/6A细胞血管新生,而VEGFR1信号可能不参与胰岛素诱导的RF/6A细胞血管新生。 

关键词: 胰岛素, RF/6A, VEGFR1, VEGFR2, 血管新生

Abstract: Objective: To study the effect of insulin on angiogenesis in RF/6A retinal vascular endothelial cells and elucidate the underlying mechanism. Methods : RF/6A cells were treated with 01nmol/L、 1nmol/L and 100 nmol/L of insulin respectively and MTS, cells migration experiment and lumen formation experiment were used to test cell proliferation, migration and lumen formation respectively,.RT-PCR and Western bot were used to measure the expression of vascular endothelial growth factor receptors VEGFR and phosphorylated receptors . Results : Insulin promoted RF /6A cell proliferation, migration and lumen formation (all P<0.01). Insulin also increased the both the expression of VEGFR2 mRNA and its phosphorylation (P< 0.01or P<0.05). There was no significant difference in the expression of VEGFR1 mRNA among the groups (P> 0.05). Conclusion Insulin might promote RF/6A angiogenesis via a VEGFR2 mediated mechanism that does not involve VEGFR1.

Key words: Insulin, RF / 6A, VEGFR1, VEGFR2, Angiogenesis