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人生长激素对人乳腺癌细胞增殖和凋亡的影响

许立生1,王水2,黄中晶1,沈恩超1,钱超1   

  1. 1. 江苏大学附属医院
    2. 南京医科大学第一附属医院
  • 收稿日期:2011-05-27 修回日期:2011-10-30 出版日期:2011-12-15 发布日期:2011-12-15
  • 通讯作者: 许立生

The effects of human growth hormone on proliferation and apoptosis of human breast neoplasm cells

  • Received:2011-05-27 Revised:2011-10-30 Published:2011-12-15 Online:2011-12-15
  • Contact: Li-Sheng XU

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摘要： 目的 探讨人生长激素对人乳腺癌细胞增殖和凋亡的影响。方法 建立乳腺癌裸鼠移植瘤模型，将40只裸鼠随机分为4组：对照组、重组人生长激素( rhGH )组、5-氟尿嘧啶(5-FU)组和rhGH+5-FU组, 各组ip给药，2周后，观察各组药物对肿瘤生长的影响。采用MTF法检测肿瘤细胞的增殖，免疫组化检测肿瘤细胞增殖，TUNEI法检测移植瘤组织的细胞凋亡。结果 与对照组相比，5-FU组和rhGH+5-FU组S期细胞、肿瘤质量及PI明显降低( P<0.05)，凋亡指数、G0/G1期细胞明显升高(P < 0. 05)；与5-FU组相比，rhGH+5-FU组的肿瘤质量、PI及G2M期下降率明显优于5-FU组， G0/G1期上升率明显优于5-FU组（P＜0.05），而rhGH 组与对照组各项指标比较无明显差异( P > 0. 05)。结论 人生长激素在体内对人乳腺癌细胞既无明显的增殖作用，也无明显的凋亡作用，但与5-FU联用后能增强MCF-7乳腺癌细胞的化疗具有协调作用。

关键词: 人生长激素, 人乳腺癌细胞, 增殖, 凋亡

Abstract: To study the effects of human growth hormone on pro liberation and apoptosis is of human breast neoplasm cells of human in vivo. Methods : The nude mice randomly were divided into four groups and each group 10 mouse. Nude mice were randomly divided into control group, recombinant human growth hormone (rhGH) group, 5-fluorouracil (5-FU) group and rhGH+ 5-FUgroup after human gastric cancer engraft model of nude ice as successfully established. IP give medicine in each group, and observe the influence of drugs on tumor growth after two weeks. The cell cycle, carryon proliferation antigen, apoptosis index (AI) of human breast cancer cell were investigated by flow cytometry, immunohistochemical detection, and terminal dioxin elatedly transferees-me dieted deoxyurdine troposphere biotin nick end labeling (TUNEL) respectively. Results: Cells in S phase, tumor quality and PCNA of gastric cancer engraft obviously degraded, but A I and G0/G1 phase obviously increased in 5-FU group and rhGH+ 5-FU groups compared with control and rhGH groups (P < 0.05); Compared with 5- FU group, rhGH + 5- FU group of tumor quality, PI and G2M period decline rates significantly better than 5- FU group, G0/G1 phase obviously better than the rate 5- FU group (P < 0.05). Between rhGH group and control group, there was no difference in above indices. Conclusion: Exogenous human growth hormone has no obvious effects on pro liberation and apoptosis of human pancreatic cancer cells in vivo. In vitro r-hGH might promote the sensitivity of chemotherapy of 5-FU to MC'F-7 cells

Key words: human growth hormone, human breast neoplasm cells, proliferation, apoptosis