天津医药

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吸入布地奈德对哮喘大鼠气道平滑肌细胞SERCA表达的影响

李淼,尚云晓   

  1. 辽宁省沈阳市和平区三好街36号中国医科大学附属盛京医院小儿呼吸内科
  • 收稿日期:2011-02-28 修回日期:2011-08-12 出版日期:2011-12-15 发布日期:2011-12-15
  • 通讯作者: 李淼

Effect of glucocorticoid inhaled on the expression of SERCA in asthmatic rat’s airway smooth muscle cell

  • Received:2011-02-28 Revised:2011-08-12 Published:2011-12-15 Online:2011-12-15

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摘要： 摘要 目的：探讨吸入布地奈德混悬液对哮喘大鼠气道平滑肌细胞（ASMC）肌浆网钙-ATP酶（SERCA）表达的影响。方法：按照随机数字分组的方法将大鼠分为正常组、哮喘组及布地奈德干预组，每组8只。哮喘组用卵蛋白雾化吸入法制作哮喘大鼠模型；正常组用PBS代替卵蛋白吸入；布地奈德干预组在卵蛋白吸入后用布地奈德混悬液泵吸干预。各组大鼠ASMC原代培养；实时定量PCR检测SERCA2mRNA于ASMC中的表达；Western blot检测SERCA2于ASMC中表达。结果：SERCA2mRNA表达及蛋白水平在哮喘组ASMC中较在正常组ASMC中降低，差异有统计学意义（P<0.05）。SERCA2mRNA表达及蛋白水平在布地奈德干预组ASMC中均较在哮喘组ASMC中增高，差异有统计学意义（P<0.05）；而与在正常组ASMC中表达相比差异无统计学意义（P>0.05）。结论：布地奈德有可能通过增加SERCA2表达促进细胞内钙离子储备，从而改善哮喘气道高反应性。

关键词: 哮喘, 肌细胞, 平滑肌, 肌浆网钙转运ATP酶类, 布地奈德, 聚合酶链反应, 大鼠, Wistar

Abstract: Abstract: Objective To investigate the role on budesonide to the expression of SERCA2 in asthmatic rat’s airway smooth muscle cell. Methods According to random method, the rats were divided into normal group, asthmatic group and budesonide intervened group. By inhaled OVA to make asthmatic rat model and primary culture ASMC; Real-time PCR and Western blot were used to detect the expression of SERCA2 in ASMC. Results The expression of SERCA2mRNA and protein in asthmatic group were less than in normal group （P < 0.05）; in budesonide intervened group the expression increased than in asthmatic group （P < 0.05）; but the expression of SERCA2mRNA and protein in budesonide intervened group compared with normal group, the difference was not statistically significant （P > 0.05）. Conclusion The expression of SERCA2 in asthmatic ASMC decreased than in normal group; budesonide could increase the expression of SERCA2mRNA, which could be benefit to promote the calcium reserve in ASMC and reduce airway hyper- responsiveness in asthma.

Key words: Wistar