天津医药

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Twist1、MMP-2及MMP-9在肝细胞肝癌血管生成中的表达及意义

陈嫱1,2,孙保存3   

  1. 1.
    2. 天津医科大学
    3. 天津医科大学，天津医科大学附属肿瘤医院
  • 收稿日期:2010-12-03 修回日期:2010-12-26 出版日期:2011-03-15 发布日期:2011-03-15
  • 通讯作者: 孙保存

Promotion of hepatocellular carcinoma angiogensis through matrix metalloproteinase activation by epithelial-mesenchymal transition regulator twist1

1,1,   

  • Received:2010-12-03 Revised:2010-12-26 Published:2011-03-15 Online:2011-03-15

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摘要： 目的：探讨上皮-间充质转变调控因子Twist1、基质金属蛋白酶（MMP）-2和MMP-9与肝细胞肝癌微血管密度（MVD）之间的关联性及临床病理意义。方法：收集97例肝细胞肝癌标本及临床病理资料，利用CD31标记MVD，应用免疫组织化学染色检测Twist1、MMP-2和MMP-9的表达，分析其表达与MVD、临床病理指标之间的关系。结果：MVD与病理分级和临床分期有关联性（P<0.05），Twist1浆表达、Twist1核表达、MMP-9表达阳性病例的MVD高于阴性组（P值均<0.05），对阳性病例进行Person相关分析亦证实Twist1浆表达、Twist1核表达、MMP-9表达与MVD存在关联性（P值均<0.05）。结论：肝细胞肝癌MVD增高伴随Twist1、MMP-9的高表达，这均对于提示预后不良具有一定的临床意义。

关键词: 上皮-间充质转变, 基质金属蛋白酶, 肝细胞肝癌, 微血管密度

Abstract: Objective: To investigate the correlation between epithelial-mesenchymal transition (EMT)regulators Twist1, matrix metalloproteinase (MMP)-2 and MMP-9, and microvessel density (MVD) in hepatocellular carcinoma and its clinical and pathological significance. Methods: A total of 97 cases of hepatocellular carcinoma specimens and their clinical data were collected. CD31 was used to mark the microvessel density (MVD). The expressions of Twist1,MMP-2 and MMP-9 were examined by immunohistochemistry staining. The correlations between the expressions, MVD and clinical and pathological indicators were analyzed. Results: MVD was correlated with clinical stage(P<0.05). The MVD of patients positive for Twist1-nuclear,Twist- cytoplasm and MMP-9 was higher than that of patients negative for Twist1-nuclear,Twist- cytoplasm and MMP-9(All P<0.05). Person correlation analysis of the positive cases also confirmed that the expressions of Twist1-nuclear,Twist- cytoplasm and MMP-9 were correlated with MVD (P<0.05).Conclusions: MVD increased was accompanied by high expression of Twist1 and MMP-9, with a certain clinical significance in indicating a poor prognosis.

Key words: epithelial-mesenchymal transition, matrix metalloproteinase, hepatocellular carcinoma, microvessel density