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α-硫辛酸对高糖作用后βTc6细胞的保护作用

李雪峰1,倪颖2,贾文红1,李忍萍1,李志臻3   

  1. 1. 河南科技大学第二附属医院
    2. 洛阳市第一人民医院
    3. 郑州大学第一附属医院
  • 收稿日期:2010-07-02 修回日期:2011-03-21 出版日期:2011-07-15 发布日期:2011-07-15
  • 通讯作者: 李雪峰

α-Lipoic acid Prevents the Decrease of MafA Gene Expression Induced by Elevated Glucose Concentrations in βTc6 Cell

  • Received:2010-07-02 Revised:2011-03-21 Published:2011-07-15 Online:2011-07-15
  • Contact: LI Xue-Feng

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摘要： 【摘要】 目的 研究高糖毒性对βTc6细胞胰岛素基因及其转录因子MafA表达的影响及α-硫辛酸的保护作用，探讨α-硫辛酸在氧化应激状态下对β细胞保护作用的机制。方法 培养βTc6细胞，分组为5mmol/L葡萄糖（NG组）、16.7mmol/L葡萄糖（HG组）、16.7mmol/L葡萄糖及200μmol/L α-硫辛酸（HG+ALA组）。培养48h后收集细胞培养液，检测胰岛素浓度并提取细胞总RNA，RT-PCR法检测胰岛素基因及转录因子MafA mRNA水平。结果 HG组与NG组比较，胰岛素mRNA的表达下降了66.2％(P<0.05)；MafA mRNA的表达下降了69.5％(P<0.01)。HG+ALA组与HG组相比，胰岛素mRNA的表达则提高了5.45倍(P<0.05)；MafA mRNA的表达提高了4.49倍(P<0.05)；而与NG组相比，胰岛素和MafA mRNA的表达差异均无统计学意义(均P >0.05)。结论 糖毒性可能是通过激活βTc6细胞内氧化应激使MafA的表达下降，而导致胰岛素基因表达下降，α-硫辛酸通过上调MafA的表达而对β细胞起到保护作用。

关键词: 糖毒性, 氧化应激, MafA, α-硫辛酸

Abstract: 【Abstract】 Objective To study the impact of elevated glucose on the expression of insulin gene and insulin transcript facor MafA，and to clarify the mechanism of the protection of α-lipoic acid for β cell under oxidative stress. Methods βTc6 cells were cultured in DMEM, and then cultured for 48 h in media containing 5mmol/L glucose (NG group)，16.7 mmol/L glucose (HG group)，or 16.7mmol/L glucose and 200μmol/L α-lipoic acid (HG+ALA group) espectively. The insulin concentration in supernatant of cells cultured in different glucose medium was assayed by RIA. The mRNA expression levels of insulin and MafA was determined by RT-PCR. Results In HG group，insulin mRNA levels were decreased by 66.2％(P<0.05)，and MafA mRNA levels decreased by 69.5％(P<0.05)，as compared with NG group．In HG+ALA group，insulin mRNA levels were increased by 5.45 fold(P<0.05)，and MafA mRNA levels by 4.49 fold(P<0.05) respectively，as compared with HG group. There was no significant difference in insulin and MafA mRNA levels between NG and HG + ALA group (P>0.05). Conclusions These data suggested that elevation in glucose concentrations led to a decrease in insulin and insulin transcript factor MafA mRNA levels in βTc6 cells . α-lipoic acid could attenuate this impact of elevated glucose through increasing the expression level of MafA.

Key words: glucotoxicity, oxidative stress, MafA, α-lipoic acid