天津地区儿童急性下呼吸道感染病毒病原学分析

天津地区儿童急性下呼吸道感染病毒病原学分析

孔梅¹,李晓燕¹,²

1. 天津市疾病预防控制中心
2.

收稿日期:2010-09-30 修回日期:2011-02-15 出版日期:2011-08-15 发布日期:2011-08-15
通讯作者: 孔梅

Virus etiology in children with acute lower respiratory tract infection in Tianjin

Received:2010-09-30 Revised:2011-02-15 Published:2011-08-15 Online:2011-08-15
Contact: mei kong

孔梅1 ,李晓燕1 ,2 . 天津地区儿童急性下呼吸道感染病毒病原学分析[J]. .

摘要/Abstract

摘要： 摘要　目的:分析天津地区儿童急性下呼吸道感染的鼻咽分泌物病毒病原学分布概况。方法:采用多重RT-PCR法,对202例鼻咽分泌物样本同时检测腺病毒（ADV），副流感病毒（PIV）1、2、3，流感病毒（Flu） A、B，鼻病毒（RhinoV），呼吸道合胞病毒（RSV） A、B，人偏肺病毒（HMPV），冠状病毒（CoV）OC43/HKU1、229E/NL63共14种常见呼吸道病毒，以及PCR法检测人博卡病毒（HBoV）和WU多瘤病毒。结果: 202份样本中检出致病性病毒阳性的有151份（74.8%） ,其中2种以上病毒混合感染54份（26.7%）; RSV检出阳性率最高为42.1%，其次为PIV（20.8%）和RhinoV（19.8%）；RSV在12月至次年3月检出率明显升高，PIV3在3月至5月成为主要病原，CoV 2种血清型均同时存在，HBoV混合感染率为61.5%；各年龄段病毒阳性检出率比较差异无统计学意义；WU多瘤病毒在下呼吸道感染患儿中检出阳性率高为19.8%。结论:RSV为天津地区儿童急性下呼吸道病毒感染主要致病原，在呼吸道感染患儿中多瘤病毒阳性检出较高，应当引起关注。

关键词: 急性下呼吸道感染, 多重PCR法, 病毒病原学

Abstract: acute lower respiratory tract infections (ALRTIs). Methods 202 NPA samples were detected by multiplex PCR for ADV, PIV1, 2, 3, Flu A, B, RhinoV A/B, RSV A, B, HMPV, Cov OC43/HKU1, Cov 229E/NL63. Human Bocavirus (HBoV) and WU polyomovirus (WUVyP) were detected by PCR. Results Among 202 NPA samples, 151 (74.8%) were pathogenic respiratory viruses positive. Of 151 virus positive patients, 54(26.7%) were 2 viruses co-infection. The most frequently detected virus was RSV(42.0%), followed by PIV and RhinoV A/B. There was a significant prevalent peak of RSV from December to March in next year. And PIV3 was the predominant pathogen from March to May. 2 serotypes of Cov co-existed in Tianjin. The most commonly co-infected virus was HBoV(61.5%). No significant age distribution of respiratory viruses was found. The prevalence of WUPyV in ALRTIs children was 19.8%. Conclusion RSV was the predominant pathogen in children with ALRTIs, We should focus on the high prevalence of WUPyV in ALRTIs.

Key words: acute lower respiratory tract infection, multiplex PCR, virus etiology