天津医药

• 实验研究 • 上一篇    下一篇

制作C57小鼠烧伤模型时Meeh常数的取值研究

廖庚进1,罗鹏飞2,付晋凤2,贾一稻2   

  1. 1. 昆明医学院第二附属医院烧伤科
    2.
  • 收稿日期:2011-04-14 修回日期:2011-05-04 出版日期:2011-08-15 发布日期:2011-08-15
  • 通讯作者: 廖庚进

The Meeh constant which should be used to estimate the body surface area of a C57BL/6 mouse in a thermal injury researching.

  • Received:2011-04-14 Revised:2011-05-04 Published:2011-08-15 Online:2011-08-15
  • Contact: Geng-Jin LIAO

PDF (PC)

2279

摘要： 摘要 目的 研究制作C57BL/6小鼠烧伤模型时适于计算全身体表面积（BSA）的Meeh常数（k）值。方法 随机选择24只6-12周正常C57BL/6小鼠雌雄各半并均分为3组。测量小鼠正常饮食时1日内的体重变化，及其正常饮食时8AM、4PM和12PM时的体重和BSA，并计算每只小鼠的k值。结果 1日内C57BL/6小鼠的体重4PM时最低，12PM最高。C57BL/6小鼠8AM时实测k值为9.617±0.104，4PM为9.802±0.110，12PM为9.570±0.244。小鼠4PM的实测k值与8AM和12PM的k值之间均有统计学差异。小鼠常用k值（9.1、9.5、10和11.4）与C57BL/6小鼠8AM或4PM的实测k值间均有统计学差异。 结论 制作C57BL/6小鼠烧伤模型时可按实验时间选用不同的k值，8AM时k值宜为9.617，4PM时为9.802。

关键词: C57BL近交系小鼠, 烧伤, 体表面积

Abstract: [Abstract] Objective To investigate the Meeh constant(k) which could be used in the Meeh Formula to estimate the body surface area(BSA) of the C57BL/6 mouse when making its thermal injury model. Methods 24 normal C57BL/6 mice of 6-12 weeks age were randomly collected and intook normal diet until the experiment day. On the 3rd day before the experiment day, each mouse was weighted in every 4 hours. The next day, the 24 mice were randomly divided into 3 groups which congtained 4 male and 4 female mice. On the experiment day, the 1st group was anaesthetized, weighed and skinned at 8AM, while the 2nd group at 4PM and the 3rd one at 12PM. The BSA and k value of each mouse was recorded. Results The average weight of the 24 C57BL/6 mice reach its botton at 4PM and its top at 12PM. The calculated k value was 9.617±0.104 at 8AM, 9.802±0.110 at 4PM, and 9.570±0.244 at 12PM. There was statistical difference not only between the k value at 8AM and 4PM, but also between 4PM and 12PM. The k value which was often quoted as 9.1, 9.5, 10 or 11.4 when the literature dealing with the Meeh Formula of mice was reviewed. There was statistical difference not only between the often-quoted k value and the calculated k value at 8AM, but also between the often-quoted k value and the calculated k value at 4PM. Conclusions In the procedure of making the thermal injury model of C57BL/6 mice， it was reliable to use 9.617 as the k value to estimate the BSA at 8AM, but use 9.802 at 4PM.

Key words: Mice, Inbred C57BL, burn, body surface area