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PIM-1基因沉默对前列腺癌细胞生长抑制作用的研究

张晓光1,徐勇2,张志宏2,杨阔3   

  1. 1. 天津市第三中心医院
    2. 天津医科大学第二医院泌尿外科
    3. 天津医科大学第二医院
  • 收稿日期:2012-01-17 修回日期:2012-06-12 出版日期:2012-11-15 发布日期:2012-11-15
  • 通讯作者: 张晓光

Effect of Silence of the PIM-1 Expression on Proliferation of Prostate Cancer Cell Line

  • Received:2012-01-17 Revised:2012-06-12 Published:2012-11-15 Online:2012-11-15

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摘要： 目的 探讨RNA干扰(RNAi)沉默PIM-1基因表达对前列腺癌细胞的生长抑制作用。方法 利用RNAi重组质粒转染前列腺癌PC-3细胞沉默PIM-1基因，进行体外研究；利用细胞计数法，检测PC-3细胞增殖能力的变化；利用流式细胞技术检测对PC-3细胞的细胞周期及凋亡的影响。结果 与对照组相比，PIM-1基因沉默可以抑制PC-3细胞的体外增殖能力，细胞周期中G1期细胞的比例升高而S期细胞的比例降低，发生早期凋亡的细胞比例明显升高。结论 PIM-1基因沉默可以使PC-3细胞的细胞周期进程受阻，抑制细胞增殖，并诱发细胞凋亡。PIM-1可以作为前列腺癌基因治疗的有效靶点，具有潜在的临床应用价值。

关键词: 前列腺癌, RNA干扰, PIM-1, 生长抑制

Abstract: Abstract Objective: To investigate the effect of RNAi-mediated silencing of PIM-1 on the proliferation of prostate cancer cell. Methods: The recombinant plasmid vectors of RNAi were transfected into PC-3 cell line in vitro and the cell-counting assay were used to determine the proliferation activity of the stably-transfected cells. Flow cytometer(FCM) were used to detect the influence of the silence of PIM-1 on cell cycle and apoptosis in PC-3 cells. Results: Compared with the contrast groups, the ability of proliferation of the PC-3 cell line with silenced PIM-1 gene was suppressed remarkably. The percentage of the cells in G1 stage increased, while which in S stage decreased. At the same time, much more apoptosis cells were observed. Conclusion: Silence of PIM-1 gene can result in the block of the cell cycle, suppression of cell proliferation and increasing apoptosis in PC-3 cells. PIM-1 may serve as a effective target of gene therapy for prostate cancer in clinical practice .

Key words: prostate cancer, RNA interference, PIM-1, proliferation suppression