天津医药

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不同频率间歇低氧对3T3-L1脂肪细胞炎症因子和脂肪因子的影响

岳晶晶1,周芹1,何庆2,朱慧1,曹竞文1,冯靖1,陈宝元3,牛文彦3,4   

  1. 1. 天津医科大学总医院
    2. 天津医科大学总医院内分泌科
    3.
    4. 天津医科大学免疫教研室
  • 收稿日期:2011-09-06 修回日期:2011-10-31 出版日期:2012-02-15 发布日期:2012-02-15
  • 通讯作者: 岳晶晶

Effect of Different Frequency of Intermittent Hypoxia on Proinflammatory Cytokines and Adipokines in 3T3-L1 Adipocytes

  • Received:2011-09-06 Revised:2011-10-31 Published:2012-02-15 Online:2012-02-15
  • Contact: Jing Jing YUE

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摘要： 摘要 目的：测定不同频率间歇低氧处理的脂肪细胞上清液内肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）和瘦素、脂联素水平的变化。方法：以分化成熟的3T3-L1脂肪细胞为研究对象，随机分为8组，即4个不同频率间歇低氧组（IH）和各自的正常氧对照组（SC）。完成暴露后收集培养基，采用ELISA法测定脂肪细胞上清液中TNF-α、IL-6、瘦素和脂联素的水平。结果：各间歇低氧组脂肪细胞上清液内TNF-α、IL-6的水平均明显高于各自的正常氧对照组（P<0.01），且IH3组显著高于其余IH组（P<0.05）；各间歇低氧组脂肪细胞上清液中瘦素的水平均明显高于各自的正常氧对照组（P<0.01），且IH3组显著高于其余IH组（P<0.05）；各间歇低氧组脂肪细胞上清液内脂联素的水平均明显低于各自的正常氧对照组（P<0.01），且IH3组显著,低于其余IH组（P<0.05）。结论：间歇低氧可以导致体外培养的脂肪细胞TNF-α、IL-6和瘦素、脂联素的分泌异常，推测脂肪细胞的内分泌功能紊乱参与了OSAHS患者的胰岛素抵抗（IR）。

关键词: 阻塞性睡眠呼吸暂停低通气综合征, 间歇低氧, 3T3-L1脂肪细胞, TNF-α, IL-6, 瘦素, 脂联素

Abstract: Abstract Objectives: To detect the levels of IL-6, TNF-α, leptin and adiponectin on the supernatant of the adipocytes, which was treated by different frequency of intermittent hypoxia. methods: Differentiated 3T3-L1 adipocytes, was randomly divided into 8 groups including: four groups which were treated by different frequence intermittent hypoxia (IH) and their sham control (SC) groups which were treated by normal oxygen. Collected the supernatant after finishing exposure, the levels of TNF-α, IL-6, leptin and adiponectin were detected by ELISA. Results: The levels of TNF-α and IL-6 of intermittent hypoxia groups were all significantly higher than the control group（P<0.01）, and the levels of TNF-α and IL-6 of IH3 group were significantly higher than other intermittent hypoxia groups（P<0.05） . The leptin levels of intermittent hypoxia groups were all significantly higher than the control group（P<0.01）, and the leptin. level of IH3 group was significantly higher than other intermittent hypoxia groups（P<0.05）.The adiponectin levels of intermittent hypoxia groups were all significantly lower than the control group（P<0.01）, and the adiponectin level of IH3 group was significantly lower than other intermittent hypoxia groups（P<0.05）. Conclusions: Intermittent hypoxia was able to induce the secretion disorder of TNF-α, IL-6, leptin and adiponectin in cultured adipocytes. It was indicated that adipocytes’endocrine dysfunction was involved with insulin resistance (IR) in OSAHS patients.

Key words: OSAHS, intermittent hypoxia, 3T3-L1 adipocyte, TNF-α, IL-6, leptin, adiponectin