天津医药

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CD64在结缔组织病儿童外周血中性粒细胞上表达的研究

李崇巍,黄敬孚,许俐,胡坚   

  1. 天津市儿童医院
  • 收稿日期:2011-05-23 修回日期:2011-11-23 出版日期:2012-02-15 发布日期:2012-02-15
  • 通讯作者: 李崇巍

A Study of CD64 Surface Expression on Neutrophils in Children with Connective Tissue Diseases

2,XU Li3, 2   

  • Received:2011-05-23 Revised:2011-11-23 Published:2012-02-15 Online:2012-02-15

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摘要： 摘要 目的：研究中性粒细胞表达CD64在小儿弥漫性结缔组织病（CTD）中的临床应用价值。 方法：共73名研究对象，分为3组：①健康对照组（n=30）；②细菌感染组（n=27），为肯定的现症细菌感染患儿，并且具有与临床相符的细菌学证据；③CTD组（n=16），包括儿童系统性红斑狼疮（SLE）11例、幼年皮肌炎（JDM）5例，均为活动性病变且临床排除了合并感染。中性粒细胞表面表达的CD64通过流式细胞仪采用QuantiBRITE技术定量测定。所得数据应用SAS System for Windows V8统计软件包进行统计学处理。 结果：健康儿童中性粒细胞表达CD64的平均分子数为1434±529，男女间没有统计学差异（1300±494vs1556±547，p>0.05）。CTD患儿中性粒细胞表达CD64平均分子数为1391±576，与对照相比没有显著性差异（P>0.05）。细菌感染患儿CD64表达为4995±2636，与对照组和CTD组相比明显上调(p<0.0001)。结论：细菌感染时中性粒细胞表达CD64增加，而在未合并感染的小儿CTD病情活动时表达不增加。定量检测中性粒细胞表达的CD64有可能成为鉴别CTD病情活动与继发细菌感染的一个实验室指标。

关键词: CD64, 结缔组织病, 感染, 细菌性, 儿童

Abstract: Abstract Objective: To study the clinical value of CD64 surface expression on neutrophils in children with connective tissue diseases(CTDs). Methods: 73 children were studied including normal controls (n=30), cases with bacterial infection (n=27) and CTDs (n=16). All patients in CTD group (11 cases of juvenile systemic lupus erythymatosus and 5 cases of juvenile dermatomyositis) were active rheumatic diseases with systemic infections excluded clinically. CD64 surface expression on neutrophils was measured quantitatively using QuantiBRITE flow cytometry technique. SAS System for Windows V8 was used to perform statistical analysis. Results: The mean antigen-per-cell of CD64 on neutrophil surface in normal controls was 1434±529, without difference between two genders（1300±494 vs 1556±547，p>0.05）. The neutrophil CD64 level was 1391±576 in patients with CTDs, showing no significance when comparing to that of normal controls (p>0.05). Compared with normal controls and patients with CTDs, the expression of CD64 increased significantly in patients with bacterial infection (4995±2636, p<0.0001). Conclusion: CD64 surface expression on neutrophils upregulates in patients with bacterial infection, however it does not elevate in active diseases of CTDs in children. It might become a potential indicator for distinguishing bacterial infection and the flare of connective tissue diseases.

Key words: CD64, connective tissue diseases, infection, bacterial, children