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Nup88和PINCH在结直肠癌中表达的关系

贺新奇,赵增仁   

  1. 河北医科大学第一医院
  • 收稿日期:2012-05-22 修回日期:2012-07-17 出版日期:2012-09-15 发布日期:2012-09-15
  • 通讯作者: 赵增仁

Relationship of Nup88 and PINCH in colorectal carcinoma

  • Received:2012-05-22 Revised:2012-07-17 Published:2012-09-15 Online:2012-09-15

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摘要： 摘要：目的 检测核孔蛋白复合体蛋白88(Nup88)和PINCH mRNA在结直肠癌组织及肿瘤细胞株中的表达水平，探讨两者之间潜在的关系。方法 利用RT-PCR的方法研究Nup88和PINCH mRNA在47例结直肠癌组织、44例癌旁无瘤粘膜、45例上残端、44例下残端粘膜及6个结肠癌细胞株中的表达水平，并进一步用Spearman法对Nup88和PINCH进行相关性分析。结果 Nup88和PINCH mRNA在结直肠癌组织、癌旁无瘤粘膜、上残端及下残端粘膜中的表达水平均呈正相关，相关系数分别为：0.31，0.35，0.46，0.48；而在细胞株中的表达水平亦有正相关趋势（r=0.6，P=0.24）。结论 Nup88和PINCH在mRNA表达水平上存在正相关关系，两者可能在结直肠癌发生发展过程中共同发挥作用。

关键词: 结直肠癌, RT-PCR, 相关分析, Nup88, PINCH

Abstract: Abstract: Objective To study the nucleoporin 88 (Nup88) and PINCH mRNA level in colorectal carcinoma and cell lines, and further to investigate the potential correlation between them. Methods Nup88 and PINCH mRNA were detected by RT-PCR in 47 colorectal carcinomas, 44 adjacent non-cancerous mucosa, 45 proximal surgical margin, 44 distal surgical margin and 6 colon cell lines. The correlation study of Nup88 and PINCH was carried out by Spearman analysis. Results Nup88 and PINCH mRNA was positive correlated in colorectal carcinoma, adjacent non-cancerous mucosa, proximal and distal surgical margin. In addition, there was a positive correlation trend between Nup88 and PINCH in colon cell lines(r=0.6, P=0.24). Conclusion Nup88 and PINCH were positive correlated at mRNA level, and they might play a role in the tumorigenesis and development of colorectal cancer.

Key words: Colorectal Carcinoma, Reverse Transcriptase Polymerase Chain Reaction, Correlation Study, nucleoporin 88, PINCH