天津医药

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牛磺酸后处理对大鼠肢体缺血再灌注后胰腺损伤的影响

郑艳妮1,门秀丽1,候阳阳1,宋旭东2,吴 静1,3,赵静2   

  1. 1. 河北联合大学基础医学院病理生理学系
    2. 河北联合大学基础医学院病理学系
    3.
  • 收稿日期:2013-04-25 修回日期:2013-06-30 出版日期:2013-12-15 发布日期:2013-12-15
  • 通讯作者: 门秀丽

The Effects of Taurine Postconditioning in Pancreatic Injury Following Limb Ischemia Reperfusion in Rats

ZHENG yan ni1,MEN Xiu li1,HOU Yang yang1,SONG Xu dong2,WU Jing 1,ZHAO Jing 2   

  • Received:2013-04-25 Revised:2013-06-30 Published:2013-12-15 Online:2013-12-15
  • Contact: MEN Xiu li

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摘要：

【摘要】  目的  观察大鼠肢体缺血再灌注（LIR）后胰腺的变化及牛磺酸（Tau）后处理的保护作用。 方法  将24只SD雄性大鼠随机分为对照（Control）组、LIR组和Tau组，每组8只。测定各组血浆黄嘌呤氧化酶（XOD）、丙二醛（MDA）、活性氧（ROS）、超氧化物歧化酶（SOD）、胰淀粉酶（AMS）、胰脂肪酶（LPS）、胰蛋白酶（Trypsin）含量的变化，光镜下观察胰腺组织形态学变化，用免疫组化观察胰腺组织中C/EBP同源蛋白（CHOP）的表达，TUNEL法观察胰腺组织细胞凋亡情况。   结果  LIR组MDA、XOD、ROS、AMS、LPS及Trypsin均高于Control组和Tau组，SOD低于Control组和Tau组（均P＜0.05）。HE染色结果显示LIR组腺泡结构破坏，间质血管扩张充血,血管周围有炎细胞浸润，胰岛形态不规则。Tau组较LIR组腺泡分界略清楚，血管扩张程度减轻，周围炎细胞减少，核浆分界和胰岛结构均有改善。免疫组化染色结果显示Tau组胰腺组织CHOP表达较LIR组下调。TUNEL染色结果显示Tau组胰腺组织细胞凋亡数量较LIR组减少。  结论  Tau后处理可减轻大鼠LIR后胰腺损伤，其机制可能与抑制氧化应激反应、下调CHOP表达，从而减轻内质网应激诱导的细胞凋亡有关。

关键词: 再灌注损伤, 胰腺, 牛磺酸, 细胞凋亡, 转录因子CHOP, 后处理

Abstract:

[Abstract]   Objective   To observe changes of the pancreas after limb ischemia reperfusion, and the protective effect
of taurine (Tau) postconditioning.  Methods   Twenty-four male SD rats were randomly divided into control group (Control), limb ischemia reperfusion (LIR) group, and taurine postconditioning (Tau) group (n=8for each group). Plasma contents of xanthine oxidase (XOD), malondialdehyde (MDA), reactive oxygen species (ROS), superoxide dismutase (SOD), pancreatic amylase (AMS), pancreatic lipase (LPS) and trypsin were detected in three groups. Morphological changes of the pancreas were observed using optical microscopy. The expressions of anti-C/enhancer-binding protein homologous protein (CHOP) in pancreas were analyzed by immunohistochemistry assay. TUNEL staining was performed to estimate the apoptosis of pancreatic cells.   Results   Compared with the control group and Tau group, plasma contents of MDA, XOD, ROS, AMS, LPS, and trypsin were significantly increased in LIR group, but the level of SOD was significantly lower in LIR group (P＜0.05). HE staining showed that acinar structure was disrupted , pancreatic lobule gap widened, stromal vascular dilatation and congestion were observed in LIR group. The perivascular infiltration of inflammatory cells appeared, islet cells were damaged with irregular islet. The immunohistochemical analysis showed the increased expression of CHOP in pancreas in LIR group than that of Tau group. And the pancreatic apoptosis was enhanced in LIR group detected by TUNEL staining.  Conclusion   Taurine postconditioning can ameliorate pancreatic injury following limb ischemia reperfusion, which may be related to the inhibition of oxidative stress, down- regulation of CHOP expression, thereby reducing endoplasmic reticulum stress- induced apoptosis.

Key words: reperfusion injury, pancreas, TAURINE, apoptosis, transcription factor CHOP, postconditioning