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康斯特液与低钾右旋糖酐液对肺动脉内皮细胞功能的影响

何立术,周琦云,胡应学,冯志强,黄强信,龚坚,李世康   

  1. 广西医科大学第一附属医院心胸外科
  • 收稿日期:2012-06-08 修回日期:2012-11-29 出版日期:2013-05-15 发布日期:2013-05-15
  • 通讯作者: 李世康

Effects of HTK and Perfadex Solution on the Function of Pulmonary Artery Endothelium

  • Received:2012-06-08 Revised:2012-11-29 Published:2013-05-15 Online:2013-05-15
  • Contact: LI Shikang

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摘要：

【摘要】 目的  探讨康斯特保护液(HTK液)与低钾右旋糖酐液(Perfadex液)低温保存对猪肺动脉内皮细胞功能的影响。方法  9只新鲜猪肺,每只取小叶间动脉(直径500～700μm)切成6条,长2mm,54条血管随机分成3组,每组18条。于4℃条件下分别用重碳酸盐缓冲液(KH液,对照组)、HTK液(实验A组)和Perfadex液(实验B组)保存4h,检测在消炎痛(7μmol/L)、N-硝基-L-精氨酸(300μmol/L)及氧合血红蛋白(20μmol/L)作用下,前列腺素F2α(1×10-7.5mol/L)引发的血管收缩反应,缓激肽、非受体介导钙离子载体引发的血管舒张反应。结果  前列腺素F2α引起的血管环最大收缩强度,缓激肽及非受体介导钙离子载体引发的血管环最大舒张反应,对照组及各实验组差异均无统计学意义(P>0.05)。结论 HTK液、Perfadex液与KH液低温保存对内皮依赖性超极化因子介导的内皮源性舒张功能的影响无明显差异。 

关键词: 肺动脉, 内皮细胞, 生物因子, 右旋糖酐类, 康斯特液

Abstract: Objective  To study the effect of HTK and Perfadex solution on the function of pulmonary artery endothelium. Methods   Fifty-four porcine pulmonary rings in 2mm long were randomly divided into three groups. Control group (n=18): Incubation in Krebs-Henseleit (KH) at 4℃ for 4 hours; group A (n=18) or B (n=18): Incubation in HTK or Perfadex solution at 4℃ for 4 hours. Endothelium-derived hyperpolarizing factor (EDHF)-mediated relaxation (percentage of -7.5logM U46619 precontraction) induced by bradykinin or calcium ionophone A23187 in the present of indomethacin (7μmol/L), L-NNA (300μmol/L) and oxyhemoglobin (20μmol/L)were measured in the organ chambers. Results   Vasoconstriction induced by U46619 is no significant difference among three groups（P>0.05）. Compared with control group, the relaxation induced by bradykinin or A23187 was no significant difference in group A or group B. Conclusions  Endothelium-derived hyperpolarizing factor (EDHF) plays an important role in endothelium-mediated relaxation of porcine pulmonary artery. EDHF-mediated relaxation showed similar performances when the lung preserved with HTK or Perfadex solution.

Key words: Pulmonary artery, endothelial cells, biological factors, dextrans, HTK solution