天津医药

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壳聚糖-左氧氟沙星长效缓释敷料的体外细胞毒性和抗菌效果研究

王晓辉1,高萍1,黄姝杰2,关静2,陶遵威1,李毅敏1   

  1. 1. 天津市医药科学研究所
    2. 军事医学科学院卫生装备研究所
  • 收稿日期:2012-08-02 修回日期:2013-03-05 出版日期:2013-06-15 发布日期:2013-06-15
  • 通讯作者: 高萍

The Cytotoxic Dressing Effects and Antimicrobial Activity of Microspheres Containing Levofloxacin

WANGXiao-Hui 1,GAO Ping 1,HUANG Shujie 2,GUAN Jing 2,TAO Zunwei 1,LI Yimin 1   

  1. 1. Institute of Medical Sciences ,Tianjin 300020，China
    2. Academy of Military Medical Sciences
  • Received:2012-08-02 Revised:2013-03-05 Published:2013-06-15 Online:2013-06-15
  • Contact: GAO Ping

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摘要： 【摘要】目的  研究壳聚糖-左氧氟沙星长效缓释敷料的体外细胞毒性、抗菌效果以及肿瘤坏死因子（TNF）-α作为评价抗菌效果指标的可行性。方法  参照ISO 10993-5:2009评价该敷料的细胞毒性；制作染菌创伤白兔模型15只并分为3组，空白对照组用凡士林油纱包扎，阳性对照组用纳米银创伤贴包扎，实验样品组用壳聚糖-左氧氟沙星长效缓释敷料包扎，于6 h、1 d、4 d、7 d，采用活组织培养法检测3组的菌落数，用双抗体夹心法检测TNF-α含量，与菌落数实验结果进行比较。结果  该敷料体外细胞毒性反应为0级；实验样品组4个时点的菌落数均低于空白对照组，在6 h、1 d、4 d时，实验样品组均低于阳性对照组（P < 0.05）；实验样品组的TNF-α含量在6 h、1 d、4 d、7 d时低于空白对照组，在6 h、1 d时显著低于阳性对照组（P < 0.05）。结论  壳聚糖-左氧氟沙星长效缓释敷料无细胞毒性，具有长效抗菌能力，TNF-α不能作为评价抗菌效果的指标。

关键词: 壳聚糖, 氧氟沙星, 迟效制剂, 肿瘤坏死因子α, 抗菌药, 细胞毒性试验

Abstract: Objective   To study the antibacterial effect of wound dressing with microspheres containing levofloxacin in the live animal experiments and it’s cytotoxicity. Methods    According to ISO 10993-5:2009, study chitosan - cytotoxic effects of wound dressing with microspheres containing levofloxacin; vaseline gauze as a blank control, nano-silver trauma attached to experimental, the dressing as the experimental group, comparing different dressings after covering the wound, the number of colonies of the wound tissue, tumor necrosis factor (TNF-α) levels. Results    The vitro cytotoxic response of the dressing was 0/0. the number of colonies in 0.25,1,4,7 D were 96 ± 9 × 105CFU / g, 435 ± 235 × 105CFU / g, 5 ± 3 × 105CFU / g, 18 to 0; ± 9 × 105CFU / g; TNF-α levels in 0.25,1,4,7 D were 44.37 ± 3.39 Pg / mL, 41.45 ± 3.28 Pg / mL, 36.07 ± 3.63 Pg / mL, 32.91 ± 5.74 Pg / mL. Conclusion     the wound dressing was no significant cytotoxicity. with the growth of administration time, reduce the number of colonies and TNF-α, the wound dressing is a long-lasting antibacterial ability of medical dressings.

Key words: chitosan, ofloxacin, delayed-action, tumor necrosis factor-alpha, Ntibacterial agent, cytotoxicity tests