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土槿乙酸对人肝癌细胞中血管内皮生长因子表达的影响

祝平   

  1. 辽宁医学院附属第一医院消化内科
  • 收稿日期:2013-07-15 修回日期:2013-09-08 出版日期:2014-01-15 发布日期:2014-01-15
  • 通讯作者: 祝平

Effect of Pseudolaric acid on the Expression of VEGF in Hepatocellular Carcinoma Cells

  • Received:2013-07-15 Revised:2013-09-08 Published:2014-01-15 Online:2014-01-15

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摘要： 目的 探讨土槿乙酸 （PAB） 对人肝癌细胞BEL-7402中血管内皮生长因子 （VEGF） 表达的影响。方法 以0 （对照组）、 0.5、 1.0、 2.0、 4.0、 8.0 μmol/L PAB处理人肝癌细胞BEL-7402， 培养6 h， 0.4%台盼蓝染色， 在显微镜下观察并计算细胞活率。采用WST-8法检测细胞增殖， RT-PCR法检测人肝癌细胞BEL-7402中VEGF mRNA表达，Western Blot检测VEGF蛋白表达。结果 不同剂量PAB处理的人肝癌细胞BEL-7402的细胞增殖率与对照组比较差异无统计学意义。随着PAB浓度的增加， VEGF mRNA表达与对照组 （2.53±0.07） 相比分别降低至1.90±0.02、 1.31±0.03、0.86±0.04、 0.60±0.03、 0.34±0.02， 差异有统计学意义 （P＜0.01）， VEGF 蛋白表达与对照组（3.06±0.15） 相比分别降低至2.60±0.09、 2.42±0.07、 1.85±0.05、 0.92±0.05、 0.60±0.04， 差异有统计学意义 （P＜0.01）。结论 PAB可抑制人肝癌细胞BEL-7402中VEGF mRNA和蛋白的表达。

关键词: 土槿乙酸, VEGF, 人肝癌细胞, RT-PCR, Western Blot

Abstract: 【Abstract】 Objective：A study was carried to investigate the impact pseudolaric acid do on VEGF expression in human liver cancer cells BEL - 7402.Methods:Human liver cancer cell BEL -7402 were processed by 0, 5, 10, 20, 40, 80 umol?L-1 pseudolaric acid separately for 6 hours, then analysed the cell proliferation and vitality in the two ways of WST 8 and trypan blue staining , and detected the VEGF expression in human liver cancer cells BEL - 7402 using the methods of RT- PCR and Western Blotting. Results: At first, compared with the control group, there was no significant difference (P > 0.05) in liver cancer cell proliferation rate and energy that processed by different doses of pseudolaric acid. Secondly with the increasing does of pseudolaric acid , VEGF protein expression was decreased in proportion, a significant difference emerged when compared with the control group (P < 0.01) and then with the increasing does of pseudolaric acid , there were significant decrease in VEGF mRNA expression compared with the control group (P < 0.01). Conclusion: Pseudolaric acid have significant inhibiting-effect on the expression of VEGF mRNA and protein in human liver cancer cells BEL - 7402 .

Key words: pseudolaric acid, VEGF, human liver cancer cells, RT-PCR, Western Blot