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SchB对UVA损伤HaCat保护作用的研究

马东东   

  1. 河北联合大学
  • 收稿日期:2013-08-07 修回日期:2013-10-23 出版日期:2014-01-15 发布日期:2014-01-15
  • 通讯作者: 马东东

Research on effects of schisandrin B cell protective effect on the long wave ultraviolet damage immortal human keratinocytes

  • Received:2013-08-07 Revised:2013-10-23 Published:2014-01-15 Online:2014-01-15

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摘要： 目的 探讨五味子乙素 （SchB） 对长波紫外线 （UVA） 诱导永生化人角质形成细胞 （HaCat细胞） 损伤后的保护作用及其可能的作用机制。方法 用 5 J/cm2的UVA照射HaCat细胞， 给予不同浓度的SchB （0.1、 0.01、 0.001、0.000 1 μmol/L） 处理UVA损伤后的HaCat细胞， 检测细胞超氧化物歧化酶 （SOD） 及谷胱甘肽过氧化物酶 （GSH-Px）的活性， 乳酸脱氢酶 （LDH） 及NO含量。结果 UVA照射HaCat细胞后， SOD、 GSH-Px活性降低， LDH及NO含量升高， 给予不同浓度的SchB均能显著提高SOD、 GSH-Px活性， 降低LDH及NO含量， 提高HaCat细胞的存活率， 尤其以0.001 μmol/L SchB的保护作用最强。结论 0.001 μmol/L SchB减轻UVA对HaCat细胞损伤的效果最佳。

关键词: 五味子乙素, 紫外线, UVA, HaCaT 细胞

Abstract: Aim to explore the schisandrin B (SchB) on the long wave ultraviolet (UVA) induced permanent resident human keratinocytes (HaCat) injury and its possi-ble mechanism. Irradiated with UVA HaCat 5Jcm2, treated with different concentrations of SchB (0.0001umol/L 0.001 umol/L 0.01 umol/L 0.1 umol/L) treatment after UVA damage of HaCat cells, the detection cell superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity, lactate dehydrogenase (LDH) and nitric oxide (ON) content. After HaCat cell was irradiated by UVA, the result of which reduced the SOD, GSH-Px activity, increased LDH and ON content, treated with different concentrations of SchB , which could increase significantly the SOD, GSH-Px activity, decrease LDH , to improve the survival rate HaCat cells, especially with the protection of the 0.001 umol/L SchB do with strongest （UVA+0.001umol/L SchB to UVA UVA model group：the cell survival rate:98.39%±3.23% to 77.110%±3.33%. GSH:8.34μmol/L±0.33μmol/L to 2.54μmol/L±0.34μmol/L, SOD:7.79 U/mL±1.22 U/mL to 3.22U/mL±0.15U/mL, LDH: 4.00 U/mL±0.98 U/mL to 7.8U/mL±1.06U/mL, NO: 0.19υmol／L±0.02υmol／L to 0.38υmol±0.03υmol , the above compared between groups p＜0.05） The conclusion is that SchB can be a cert ain extent and reduce the UVA damage of HaCat cells. As the concentration decreased, its protective effect gradually increase, when it is reaching the optimum concentration (0.001 umol/L), the protective effect of reducing mechanism, oxidative damage and inhibition of SchB cells enhance the ability of anti.

Key words: schisandrin B, Ultraviolet, long wave ultraviolet, human keratinocytes