天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (12): 1361-1364.doi: 10.11958/j.issn.0253-9896.2015.12.005

• 细胞与分子生物学 • 上一篇    下一篇

复方甘草酸苷对HaCaT 细胞表达IL-2、IL-4 的影响

尹翠红，车雅敏△   

  1. 天津医科大学总医院皮肤科(邮编300052)
  • 收稿日期:2015-03-20 修回日期:2015-06-27 出版日期:2015-12-15 发布日期:2015-12-11
  • 通讯作者: △通讯作者E-mail：lily_alan@sohu.com E-mail:lily_alan@sohu.com
  • 作者简介:尹翠红（1981），女，硕士在读，主要从事银屑病的发病机制与治疗的研究
  • 基金资助:
    中华医学会皮肤性病学分会美能皮肤病学研究基金（SNMC-201201）

Effects of glycyrrhizin on the expressions of IL-2 and IL-4 in HaCaT cells

YIN Cuihong, CHE Yamin△   

  1. Department of Dermatology, Tianjin Medical University General Hospital，Tianjin 300052, China
  • Received:2015-03-20 Revised:2015-06-27 Published:2015-12-15 Online:2015-12-11
  • Contact: △Corresponding Author E-mail:lily_alan@sohu.com E-mail:lily_alan@sohu.com

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摘要： 目的观察复方甘草酸苷(CG)对角质形成细胞株HaCaT 细胞增殖的抑制作用及其表达白细胞介素（IL）- 2、IL-4 的影响，探讨CG 治疗银屑病的作用机制。方法将培养的HaCaT 细胞分为4 组：空白对照组（不加CG）；低浓度组（1 mL 细胞培养液中加入50 μL CG）；中浓度组（1 mL 细胞培养液中加入100 μL CG）；高浓度组（1 mL 细胞培养液中加入200 μL CG）。采用噻唑蓝（MTT）方法检测24 h 后不同浓度CG 对HaCaT 细胞增殖的抑制作用；双抗体夹心酶联免疫吸附试验(ELISA)分析不同浓度CG 处理后的HaCaT 细胞上清液中IL-2 及IL-4 的表达水平。结果低、中、高浓度CG 干预HaCaT 细胞24 h 后均可见到明显的增殖抑制作用。不同浓度CG 干预HaCaT 细胞24 h 后，低浓度组上清液中IL-2 的浓度与空白对照组无明显差异（ng/L：234.51±17.98 vs 225.31±16.23，P＞0.05）；中、高浓度组的IL-2 浓度明显低于空白对照组及低浓度组，且高浓度组低于中浓度组（ng/L：188.99±19.22 vs 208.49±18.40, P＜ 0.05）；高浓度组培养上清液中IL-4 的浓度较其余3 组明显升高（ng/L：45.67±10.29 vs 37.62±3.90、39.68±6.08、43.85± 10.26，P＜0.05）。结论CG 可能是通过调节细胞因子IL-2 与IL-4 的分泌而抑制皮肤角质形成细胞的增殖。

关键词: 复方甘草酸苷, HaCaT 细胞, 白细胞介素-2, 白细胞介素-4, 细胞增殖

Abstract: Objective To observe the effect of glycyrrhizin (CG) on the proliferation and the expressions of interleukin (IL)-2, IL-4 in human keratinocytes cell line HaCaT cells, and to investigate the therapeutic mechanism of CG in the pa⁃ tients with psoriasis. Methods The HaCaT cells were divided into four groups: blank group (without CG), low concentration group (50 μL CG/mL cell supernatant), medium concentration group (100 μL CG/mL cell supernatant) and high concentra⁃ tion group (200 μL CG/mL cell supernatant). Methyl thiazolyl tetrazolium (MTT) assay was performed to evaluate the prolifer⁃ ation of HaCaT cells that were treated with CG at different concentrations respectively for 24 hours. The contents of IL-2 and IL-4 in HaCaT cells were examined by enzyme linked immunosorbent assay (ELISA). Results Compared with the blank group, there were obviously inhibitory effects in the proliferation of HaCaT cells treated by low，medium and high concentra⁃ tions of CG for 24 hours. There was no significant difference in IL-2 level between low concentration CG group and blank group (ng/L：234.51±17.98 vs 225.31±16.23, P > 0.05). After 24 hours of intervention with CG of medium and high concen⁃ trations, the expression levels of IL-2 was significantly lower in HaCaT cells than that in blank group and low concentration group. The IL-2 level was significantly lower in high concentration group than that in medium concentration group (ng/L: 188.99±19.22 vs 208.49±18.40, P＜0.05). At the same condition, the secretion of IL-4 in HaCaT cells was significantly upregulated in high concentration group than that in other three groups (ng/L: 45.67±10.29 vs 37.62±3.90, 39.68±6.08, 43.85± 10.26, P＜0.05). Conclusion Glycyrrhizin may suppress the proliferation of human skin keratinocytes by regulating secre⁃ tion of cytokines IL-2 and IL-4.

Key words: compound glycyrrhizin, HaCaT cell, interleukin-2, interleukin-4, cell proliferation