天津医药

• 细胞与分子生物学 • 上一篇    下一篇

无羁萜-3β-醇抑制自身免疫性脑脊髓炎的作用研究及其机制探讨

郭喜霞1,杨静2,黄宁1,李鹏1   

  1. 1. 新乡医学院
    2. 濮阳市卫生学校
  • 收稿日期:2014-01-27 修回日期:2014-06-04 出版日期:2014-10-15 发布日期:2014-10-15
  • 通讯作者: 李鹏

Inhibition Effect of Non Custodial Terpenes -3 β - Alcohol to Autoimmune Encephalomyelitis

  • Received:2014-01-27 Revised:2014-06-04 Published:2014-10-15 Online:2014-10-15

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摘要：

【摘要】目的 研究无羁萜-3β-醇对实验诱导的豚鼠自身免疫性脑脊髓炎（AE）的抑制作用。方法 用25、50 和100 mg/kg 剂量的无羁萜-3β-醇对实验诱导的AE 豚鼠进行灌胃8 周。分别检测血浆CD4+/CD8+，白细胞介素（IL）-1、2、6、10，血浆神经肽Y（NPY）、β-内啡肽（β-EP），脑组织转化生长因子（TGF）-β、基质金属蛋白酶（MMP）-2、一氧化氮合酶（NOS）及白细胞分化抗原CD3 的含量。光镜观察豚鼠大脑神经元形态学变化，电镜观察神经元超微结构变化，免疫荧光观察NOS 在神经元的表达。结果 无羁萜-3β-醇抑制实验诱导的AE 豚鼠CD4+/CD8+的升高，抑制血浆IL-1、2、6、10 的含量升高，抑制血浆NPY 升高及β-EP 降低，抑制大脑TGF-β降低和MMP-2、CD3 升高以及 NOS 在神经元胞质内的表达，并维持神经元的正常形态。结论 无羁萜-3β-醇具有抑制AE 的作用。

关键词: 无羁萜-3β-醇, 自身免疫, 一氧化氮合酶, 脑脊髓炎, 白细胞介素类

Abstract:

[Abstract] Objective? To study the inhibition effect of non custodial terpenes -3 β - alcohol to experimentally in? duced autoimmune encephalomyelitis in guinea pigs. Methods? Different doses (25 mg/kg, 50 mg/kg and 100 mg/kg) of non custodial terpenes -3 β - alcohol were given to the experimentally induced autoimmune encephalomyelitis model of guinea pigs by gavage for 8 weeks. Plasma levels of CD4+/CD8+, IL-1, IL-2, IL-6, IL-10, neuropeptide Y (NPY), beta endorphin (β - EP) , transforming growth factor - β (TGF- β), matrix metalloproteinase (MMP-2), nitric oxide synthase (NOS) and leuko? cyte differentiation antigen CD3 were assessed. The brain neuron morphology changes was observed under light microscopy while its ultrastructure changes was observed under electron microscope. NOS expression in neurons was observed through immunofluoresce technology. Results ?Non custodialterpenes -3β- alcohol inhibited the increase of plasma CD4+/CD8+, IL- 1, IL-2, IL-6, IL-10, MMP-2, CD3 and NPY while decrease of plasma β -EP, brain TGF- β. It also increase NOS expres? sion in neuronal cytoplasm and maintained neuron morphology. Conclusion Non custodial terpenes -3 β - alcohol inhibit? ed the experimental autoimmune encephalomyelitis in guinea pig.

Key words: non custodial terpenes -3 β - alcohol, autoimmune, nitric oxide synthase, encephalomyeliti, interleukins