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多重耐药鲍曼不动杆菌临床分布及外排泵基因型的检测

孙静娜   

  1. 河北医科大学第一医院检验科
  • 收稿日期:2014-07-02 修回日期:2014-07-29 出版日期:2014-11-15 发布日期:2014-11-15
  • 通讯作者: 孙静娜

Clinical Distribution and the Detection of Multiple Drug-Resistant Acinetobacter Baumannii Efflux Pump Genotypes

SUN Jing na   

  1. Clinical Laboratory， the First Hospital of Hebei Medical University
  • Received:2014-07-02 Revised:2014-07-29 Published:2014-11-15 Online:2014-11-15
  • Contact: SUN Jing na

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摘要：

【摘要】 目的 了解多重耐药鲍曼不动杆菌临床分布并检测其外排泵基因型。 方法 统计 96 株多重耐药鲍曼不动杆菌临床分布， 用 Kirby-Bauer 法检测 96 株多重耐药鲍曼不动杆菌对 15 种抗菌药物的耐药情况， 并用 PCR扩增进行外排泵基因的检测。 结果 96 株多重耐药鲍曼不动杆菌主要分布在重症监护病房（54.2%）和呼吸内科（18.8%）； 对喹诺酮类、头孢菌素类、氨基糖苷类、四环素类抗菌药物的耐药率均在 70%以上； 分布在重症监护病房的52 株多重耐药鲍曼不动杆菌中检测到 adeB、 adeR、 adeS、 adeJ、 adeE、 abeM 基因分别有 18 株（34.62%）、 16 株（30.77%）、18 株（34.62%）、18 株（34.62%）、0 株、18 株（34.62%）； 分布在呼吸内科的 18 株多重耐药鲍曼不动杆菌中检测到 adeB、adeR、adeS、adeJ、adeE、abeM 基因分别有 9 株、8 株、8 株、8 株、0 株、8 株。 结论 外排泵基因是分布在重症监护病房和呼吸内科的鲍曼不动杆菌发生多重耐药的重要因素。

关键词: 鲍氏不动杆菌, 微生物敏感性试验, 多重耐药, 外排泵基因

Abstract:

[Abstract]   Objective   To study the clinical distribution and detection of the efflux pump gene in multiple drug-re?
sistant acinetobacter baumannii.   Methods   The clinical distribution of 96 strains of multiple drug-resistant acinetobacter baumannii was analyzed. K-B method was used to detect 96 strains of multi resistant bauman resisted to 15 kinds of antibiotics. PCR amplification was used to detect the efflux pump gene.   Results   Ninety-six strains of multiple drug-resistant acinetobacter baumannii mainly distributed in intensive care unit (ICU, 54.2%) and respiratory department (18.8%). The drug resistance rates to quinolone, cephalosporins, amino glucoside, tetracycline were above 70%. The 52 strains of multiple drug -resistant acinetobacter baumannii detected in ICU included 18 strains of adeB (34.62%), 16 strains of adeR (30.77%),18 strains of adeS (34.62%), 18 strains of adeJ (34.62%), 0 strain of adeE and18 strains of adeM (34.62%). The18 strains of multiple drug-resistant acinetobacter baumannii detected in respiratory department included 9 strains of adeB, 8 strains of adeR, 8 strains of adeS, 8 strains of adeJ, 0 strain of adeE and 8 strains of adeM.   Conclusion   Efflux pump genes are important factors for multiple drug-resistant acinetobacter baumannii distributed in ICU and respiratory department.

Key words: acinetobacter baumannii, microbial sensitivity tests, multiple drug resistant, efflux pump gene