天津医药

天津医药 ›› 2014, Vol. 42 ›› Issue (12): 1183-1185.doi: 10.3969/j.issn.0253-9896.2014.12.009

• 实验研究 • 上一篇    下一篇

纳洛酮对右美托咪定麻醉小鼠催醒作用的研究

李翠1,2,余剑波1,2,周汾3,陈慧荣1,2,李翠1,2,宫丽荣1,2,穆蕊4,董树安1,2   

  1. 1. 天津市南开医院
    2. 天津市南开医院
    3. 天津市南开区三纬路122号南开医院麻醉科
    4. 天津南开医院麻醉科
  • 收稿日期:2014-04-28 修回日期:2014-07-28 出版日期:2014-12-15 发布日期:2014-12-15
  • 通讯作者: 李翠 E-mail:1532669162008@live.cn
  • 基金资助:
    天津医学会麻醉学分会中青年科研培育基金项目

Awaken Effect of Naloxon in Dexmedetomidine-Anesthetized Mice

li cui1,YU Jianbo 1,ZHOU Fen 2,CHEN Huirong 1,cui LI1,GONG Lirong 1,MU Rui, 1,DONG Shuan 1   

  1. 1. Deportment of Anesthesiology， Tianjin Nankai Hospital
    2. Deportment of Anesthesiology， Tianjin Nankai Hospital，
  • Received:2014-04-28 Revised:2014-07-28 Published:2014-12-15 Online:2014-12-15
  • Contact: li cui E-mail:1532669162008@live.cn

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摘要：

【摘要】 目的 探讨纳洛酮对右美托咪定麻醉小鼠有无催醒作用及其作用机制。 方法 30 只清洁级昆明种小鼠随机分成纳洛酮组（NAL 组）、阿替美唑组（ATI 组）和生理盐水组（NS 组）3 组(n=10)。 各组小鼠均腹腔注射右美托咪定 1 mg/kg， 给药后 90 min， 各组分别腹腔注射纳洛酮 2 mg/kg、阿替美唑 2 mg/kg 与生理盐水 10 mL/kg， 于右美托咪定给药前及给药后 5、15、30、60、90、95、105、120、180 min 评估镇静、镇痛水平， 并观察小鼠苏醒时间（以翻正反射恢复为标准）。 结果 各组在右美托咪定给药 5 min 后即表现明显的镇静、镇痛效应， 约在 60 min 时达到峰效应和在 180 min 恢复； 阿替美唑和纳洛酮均可降低右美托咪定的镇静、镇痛效应。 NAL 组在给药后 95、105、120、180 min 镇静评分均低于 NS 组， 在 95、105、120 min 镇静评分均高于 ATI 组； NAL 组在给药后 95、105、120 min 镇痛评分均低于 NS 组(均 P＜ 0.05)， 与 ATI 组差异均无统计学意义。 ATI 组、NAL 组小鼠的苏醒时间短于 NS 组[min： (3.0±1.9) vs (5.0± 2.5) vs (91.0±5.7)； F=1 793.368， P＜ 0.05]， NAL 组与 ATI 组差异无统计学意义。 结论 纳洛酮对右美托咪定麻醉小鼠具有一定的催醒作用。

关键词: 麻醉, 纳洛酮, 右美托咪定, 镇痛, 阿替美唑, 催醒作用, 苏醒时间

Abstract:

[Abstract] Objective To investigate the awaken effect of naloxon on dexmedetomidine anesthetized mice and its mechanism. Methods Thirty Kunming mice of clean grade were randomly divided into 3 groups which included NAL group (Naloxon group), ATI group（Atipamezole group） and NS group (Normal Saline group). All groups were given dexme? detomidine 1 mg· kg-1 intraperitoneally. Naloxon 2 mg· kg-1, atipamezole 2 mg· kg-1 and normal saline 10 mL· kg-1 were ran? domly given intraperitoneally to the NAL, ATI and NS group respectively 90 minutes after dexmedetomidine administration. At timepoints prior to dexmedetomidine administration and 5, 15, 30, 60, 90, 95, 105, 120, 180 minutes after it, the sedative and analgesic effects besides recovery time (based on restore of righting reflex loss) were assessed. Results Sedation and analgesia effects became apparent within 5 minutes, and peaked at approximately 60 minutes then spontaneously recovered at 180 minutes after injection of dexmedetomidine. The sedative and analgesic effects were reduced in both ATI and NAL groups. Compared with ATI group, the sedation scores were higher at 95, 105 and 120 minutes after dexmedetomidine admin? istration than those in NAL group (P＜ 0.05) but the scores were not statistically significant at 180 minutes between these two groups. Compared with NS group, the sedation scores were lower at time points of 95, 105, 120 and 180 minutes than those in NAL group (P＞ 0.05). The analgesic scores were not statistically significant at time points of 95, 105, 120 and 180 min? utes between NAL group and ATI group, but they were lower in NAL group compared with NS group at timepoints of 95, 105 and 120 minutes (P > 0.05). The recovery time in ATI and NAL group were shorter than that in NS group (F=1 793.368, P＜ 0.05), but it showed no statistical difference between ATI group and NAL group (P＞ 0.05). Conclusion Naloxone had a certain awaken effect on dexmedetomidine anesthetized mice.

Key words: Anesthesia, naloxone, dexmedetomidine, atipamezole, wakening effect, recovery time