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AKT、ERK1/2在下咽鳞状细胞癌中的表达及两者的相关性研究

杨珂,谷京城   

  1. 辽宁医学院附属第一医院
  • 收稿日期:2013-06-04 修回日期:2013-11-01 出版日期:2014-03-15 发布日期:2014-03-15
  • 通讯作者: 杨珂

The Expression Of AKT And ERK1/2 Protein In Hypopharyngeal Squamous Cell Carcinoma And The RelationshipBetween Them

  • Received:2013-06-04 Revised:2013-11-01 Published:2014-03-15 Online:2014-03-15
  • Contact: ke YANG

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摘要： 摘要 目的：探讨AKT和ERK1/2蛋白在下咽鳞状细胞癌组织及正常下咽粘膜组织中的表达，并分析下咽癌临床和病理因素的关系。方法：采用免疫组织化学SP法检测60例下咽鳞状细胞癌组织及15例正常下咽粘膜组织中AKT和ERK1/2的表达情况，并分析两种检测物与下咽癌的关系。结果：在60例下咽鳞状细胞癌组织中AKT阳性表达率为78.3%(47/60)，明显高于15例正常下咽粘膜组织中13.3%(2/15)的阳性率 (P＜0.05)； ERK1/2的阳性表达率为66.7%(40/60)，明显高于正常组的6.7%(1/15)，差异具有统计学意义(P＜0.01)；经统计分析AKT 和ERK1/2的表达与病理分级、临床分期及有无淋巴结转移有关，而与性别、年龄无关。结论：AKT和ERK1/2在下咽鳞状细胞癌中均高度表达，促进下咽癌发生、发展和浸润转移。两者的表达呈显著正相关(r=0.400,P＜0.05)，提示两者存在协同作用。

关键词: 下咽鳞状细胞癌, AKT, ERK1/2, 免疫组织化学法

Abstract: Abstract Objective:To investigate the expression of AKT and ERK1/2 protein in hypopharyngeal squamous cell carcinoma and normal hypopharyngeal mucosa, and their relationship with clinical and pathological characters. Methods:The expression of AKT and ERK1/2 protein in 60 patients with hypopharyngeal squamous cell carcinoma, 15 cases of normal hypopharyngeal mucosa were examined by immunohistochemical S-P technique. At the same time, relationship between AKT and ERK1/2 in hypopharyngeal squamous cell carcinoma was explored. Results:The Positive rate of the expression of AKT and ERK1/2 in 60 cases of hypopharyngeal squamous cell carcinoma is 78.3%(47/60) and 66.7%(40/60),the rate in 15 cases of normal hypopharyngeal mucosa is 13.3%(2/15) and 6.7%(1/15). There were significant differences among the expression of AKT and ERK1/2 in hypopharyngeal squamous cell carcinoma and normal hypopharyngeal mucosa. The expression of AKT in hypopharyngeal squamous cell carcinoma group was higher than that in normal groups (P＜0.05). The expression of ERK1/2 in hypopharyngeal squamous cell carcinoma group was higher than that in normal groups (P＜0.01). There was a positive correlation between the expression of AKT, ERK1/2 and the degrees of differentiation, clinical stage and pelvic lymph node metastasis, but not correlated with gender and age. Conclusion:The activation of AKT protein and ERK1/2 protein were correlated with carcinogenesis，progression，invasion and metastasis of IHSCC, The expression level of AKT and ERK1/2 have obvious positive relationship(r=0.400,P＜0.05), suggesting that there is synergistic effect between them.

Key words: IHSCC, AKT, ERK1/2, Immunohistochemistry