天津医药

• 细胞与分子生物学 • 上一篇    下一篇

人结肠癌细胞中CacyBP/SIP核转位差异表达基因的验证

王安萍1,赵盈盈2,刘欣2,翟惠虹3   

  1. 1. 宁夏医科大学
    2. 宁夏医科大学研究生院
    3. 宁夏医科大学总院消化内科
  • 收稿日期:2013-12-11 修回日期:2014-02-24 出版日期:2014-05-15 发布日期:2014-05-15
  • 通讯作者: 翟惠虹

Verification of differential expression genes after CacyBP/SIP unclear translocation in colon carcinoma cell lines

  • Received:2013-12-11 Revised:2014-02-24 Published:2014-05-15 Online:2014-05-15

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摘要： [摘要]目的 验证细胞周期PCR芯片筛选出来的差异表达基因；方法 胃泌素刺激的SW480细胞作为实验组，无胃泌素刺激的SW480细胞作为对照组。用Western blot对胃泌素刺激前后的CacyBP/SIP的表达定位进行检测；用实时荧光定量PCR技术对细胞周期PCR芯片筛选出的差异表达基因CDK8和CKS2进行验证。结果 胃泌素刺激前CacyBP/SIP主要表达于细胞质，刺激后可同时表达在细胞质和细胞核；同细胞周期PCR芯片结果一致，基因CDK8和CKS2在实验组的表达量较对照组明显上调(p≤0.05)。结论 胃泌素刺激可使CacyBP/SIP发生核转位；基因芯片筛选出的差异基因大体是可靠的，对筛选出的差异基因可以进行进一步研究。

关键词: CacyBP/SIP, 核转位, 差异表达基因, 胃泌素

Abstract: [Abstract] Object To verify the genes screend by the PCR chip of cell cycle. Methods The colon cancer cells were randomized into two groups. The test group recoved the stimulation of gastrin,while the control group not .The method of western blot were used to detect the expression of Cacybp/SIP before and after the stimulation of gastrin. Verification of differential expression of CDK8, CKS2 by using real-time quantitative PCR. Results It is found that before the stimulation of human gastrin, CacyBP/SIP is located and expressed in the cytoplasm, and then in both cytoplasm and nucleus after the stimulation of human gastrin. The results of real-time quanntitative PCR of CDK8 and CKS2 gene were consistent with those of microarray detection , The expressions of CDK8 and CKS2 were up-regulated. Conclusion The stimulation of human gastrin can lead to the nuclear translocation of CacyBP/SIP. The results of microarray are reliable and differentially expressed genes screened through gene chip deserve futher study.

Key words: CacyBP/SIP, nuclear translocation, differential expression gene, gastrin