天津医药

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康脑液对局灶性脑缺血再灌注大鼠Pi3kmRNA与AktmRNA的影响

肖志娟1,赵志敏1,薛茜1,邹玉安1,邢立强2   

  1. 1. 河北北方学院附属第一医院
    2. 河北北方学院
  • 收稿日期:2013-10-29 修回日期:2014-01-11 出版日期:2014-05-15 发布日期:2014-05-15
  • 通讯作者: 肖志娟

Effects of Kangnao liquid on expression of Pi3kmRNA and AktmRNA with Focal Cerebral Ischemic-reperfusion Injury in rats

  • Received:2013-10-29 Revised:2014-01-11 Published:2014-05-15 Online:2014-05-15

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摘要： 目的：观察康脑液对SD大鼠脑缺血再灌注（I/R）后的治疗效果并研究其对I/R大鼠Pi3kmRNA及AktmRNA的影响。方法：180只雄性SD大鼠随机分为6组：假手术组、模型组、康脑液高、中、低剂量组和尼莫地平组。药物治疗组分别给予康脑液（24g.kg-1.d-1、12g.kg-1.d-1、6g.kg-1.d-1），尼莫地平（1mg.kg-1d-1），每天灌胃1次，其他组每天给予等量的蒸馏水，给药7天后，除假手术组外，线栓法制备大鼠右侧大脑中动脉闭塞模型。大鼠缺血2h，于再灌注24h后观察各组神经功能，再灌注12h、24h、48h、72h、168h原位杂交技术观察各组大鼠Pi3kmRNA、AktmRNA表达。结果：与模型组相比，康脑液各剂量组神经功能明显改善，Pi3kmRNA及AktmRNA的表达明显高于模型组，尼莫地平组Pi3kmRNA及AktmRNA的表达也明显高于模型组，但较康脑液高、中剂量组低。结论：康脑液能治疗脑缺血再灌注大鼠，其机制可能是其促进脑缺血再灌注大鼠缺血周围区域Pi3kmRNA及AktmRNA表达，从而实现其对脑组织的保护作用。

关键词: 康脑液, 脑缺血再灌注, Pi3k/akt通路

Abstract: Objective To observe the therapeutical effect of kangnao liquid in rats with focal cerebral ischemia-reperfusion(I/R) and to investigate the expression of Pi3kmRNA and AktmRNA in rats.Methods 180 healthy male SD rats were randomly divided into 6 groups:sham operated group,model group,The high-,middle- and low-dose kangnao liquid groups and Nimodipine group.Rats in drug treatment groups were administrated with kangnao liquid in accordance with 24g.kg-1.d-1 and 12g.kg-1.d-1 and 6g.kg-1.d-1 and with Nimodipine in accordance with 1mg.kg-1.d-1.Rats in model and sham groups were treated with distilled water with the equal volume.Continuously administrated for 7 days, the animal models of middle cerebral artery occlusion (MCAO) were established by a monofilament method from right internal carotid artery.Neurological evaluation was performed 24h after reperfusion.The In situ hybridization was used to investigate the expression of Pi3kmRNA and AktmRNA in rats on 12h, 24h, 48h, 72h and 168h after ischemia for 2h.Results Compared with model group,kangnao liquid groups had significantly improved neurological responses.The expression of Pi3kmRNA and AktmRNA at different time points of brain tissue were significantly improved.Nimodipine group had also improved neurological responses and the expression of Pi3kmRNA and AktmRNA,but it was lower than high- and middle-dose kangnao liquid groups.Conclusions Kangnao liquid can treated the rats with cerebral ischemia-reprefusion injury and protect nerve cells of cerebral infarction areas.The protective effect of kangnao liquid may be associated with that kangnao liquid can incresse the expression of Pi3kmRNA and AktmRNA.

Key words: kangnao liquid, Cerebral ischemia reperfusion injury, Pi3k/akt pathway