天津医药

天津医药 ›› 2017, Vol. 45 ›› Issue (12): 1253-1256.doi: 10.11958/20170491

• 实验研究 • 上一篇    下一篇

康脑液对大鼠脑缺血再灌注损伤后海马 LC3、 Beclin 1 表达的影响

石会娟 1， 李凡 1， 薛茜 2， 邹玉安 2△， 田丽霞 1， 常青 2， 秦立鹏 1， 杨会欣 1， 马强 1   

  1. 基金项目： 河北省二〇〇九年医学科学研究重点计划项目 （20090591） 作者单位： 1 河北省新乐市医院神经内科 （邮编 050700）； 2 河北北方学院附属第一医院 作者简介： 石会娟 （1983）， 女， 硕士研究生， 主治医师， 主要从事缺血性脑血管病的治疗方面研究 △通讯作者 E-mail: zya8857111@sohu.com
  • 收稿日期:2017-04-19 修回日期:2017-10-20 出版日期:2017-12-15 发布日期:2017-12-15
  • 通讯作者: 石会娟 E-mail:stonejuan@163.com

Effects of kangnao liquid on the expressions of LC3 and Beclin 1 in hippocampus after cerebral ischemia-reperfusion in rats

SHI Hui-juan1, LI Fan1, XUE Qian2, ZOU Yu-an2△, TIAN Li-xia1, CHANG Qing2, QIN Li-peng1, YANG Hui-xin1, MA Qiang1   

  1. 1 Department of Neurology, Xinle Hospital, Shijiazhuang 050700, China; 2 The First Affiliated Hospital of Hebei North University △Corresponding Author E-mail: zya8857111@sohu.com
  • Received:2017-04-19 Revised:2017-10-20 Published:2017-12-15 Online:2017-12-15

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摘要： 目的 观察康脑液对脑缺血再灌注损伤大鼠海马中 LC3、 Beclin 1 的影响， 探讨其保护机制。方法 48 只 雄性 SD 大鼠按照随机数字表法分为假手术组、 模型组、 康脑液组 ［14.30 g/ （kg·d）］ 及依达拉奉组 ［10.00 mg/ （kg·d）］， 每组 12 只。康脑液组和依达拉奉组采用灌胃法给药， 假手术组及模型组灌胃等体积生理盐水。给药 7 d 后除假手 术组外， 其余各组采用线栓法制作大脑中动脉阻断（MCAO）模型， 缺血 2 h 后再灌注 24 h。再灌注结束后各组随机 抽取 6 只观察神经功能状态， TTC 染色观察脑组织形态并计算梗死百分比。剩余动物采用 HE 染色观察大鼠脑皮质 及海马神经细胞形态， 免疫组化染色法检测海马组织中 LC3、 Beclin 1 的表达情况。结果 与假手术组比较， 模型组 神经功能缺损症状明显， 脑梗死百分比显著升高， 大脑皮质和海马神经细胞变性坏死明显， 周围间质出现水肿， 海马 组织中 LC3、 Beclin 1 的表达显著上调 （P＜0.05）。与模型组比较， 康脑液组及依达拉奉组神经功能缺损症状明显减 轻， 脑梗死百分比降低， 神经元形态改变较轻， 海马组织中 LC3、 Beclin 1 表达降低 （均 P＜0.05）。结论 康脑液可能 通过降低大鼠脑缺血再灌注后海马组织中自噬相关蛋白的表达， 从而改善脑缺血再灌注损伤， 起到脑保护作用。

关键词: 脑缺血, 再灌注损伤, 大鼠, Sprague-Dawley, 康脑液, LC3, Beclin 1

Abstract: Objective To observe the effect of kangnao liquid on the expressions of LC3 and Beclin 1 in hippocampus after cerebral ischemia-reperfusion in rats, and to discuss the mechanism. Methods A total of 48 male SD rats were randomly divided into four groups: sham-operation group, model group, kangnao liqiud group [14.30 g/(kg·d)] and edaravone group [10.00 mg/(kg·d)], 12 rats in each group. The rats in kangnao liqiud group and edaravone group were administrated by intragastric administration. The rats in sham-operation group and model group were administrated with equal volume of normal saline. After treating for 7 days, except for the sham-operation group, the middle cerebral artery occlusion (MCAO) model was made by suture method in the other three groups. After 2 h of ischemia, the rats were reperfused for 24 h. Six rats in each group were randomly selected for observing the neurological function. TTC staining was used to observe the morphology of the brain tissue and calculate the percentage of infarction. The morphology of the cerebral cortex and hippocampus was observed by HE staining in the remaining animals, and the expressions of LC3 and Beclin 1 in the hippocampus were detected by immunohistochemical staining. Results Compared with the sham-operation group, model group showed an obvious neurological deficit, and the percentage of cerebral infarction increased significantly. At the same time, nerve cells of cerebral cortex and hippocampus revealed degeneration and necrosis, stroma edema, and the expressions of LC3 and Beclin 1 in hippocampus significantly increased (P＜0.05). Compared with the model group, the neurological deficit symptoms and the percentage of cerebral infarction significantly reduced, changes of neuron morphology were lighter, and the expressions of LC3 and Beclin 1 decreased significantly in kangnao liqiud group and edaravone group (P＜0.05). Conclusion Kangnao liquid shows protective effects on cerebral ischemia-reperfusion, which may be related with the decreased expressions of LC3 and Beclin 1 in hippocampus after cerebral ischemia-reperfusion in rats.

Key words: brain ischemia, reperfusion injury, rats, Sprague-Dawley, kangnao liquid, rats, LC3, Beclin 1