天津医药

• 细胞与分子生物学 •    下一篇

小檗碱对3T3-L1脂肪细胞炎症因子、脂肪因子及脂肪酸代谢的影响

李萍1,岳晶晶2,张达3,何庆1   

  1. 1. 天津医科大学总医院内分泌科
    2. 安徽省五河县人民医院内2科
    3. 天津医科大学免疫系
  • 收稿日期:2013-12-24 修回日期:2014-02-23 出版日期:2014-06-15 发布日期:2014-06-15
  • 通讯作者: 李萍

Effects of berberine on inflammatory factors, adipokines and fatty acid metabolism in 3T3-L1 adipocytes

  • Received:2013-12-24 Revised:2014-02-23 Published:2014-06-15 Online:2014-06-15
  • Contact: Ping LI

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摘要： 目的 观察小檗碱对3T3-L1脂肪细胞炎症因子、脂肪因子及脂肪酸代谢的影响，探讨小檗碱改善胰岛素抵抗的分子机制。 方法 3T3-L1脂肪细胞经不同浓度小檗碱（0μmol/L、5μmol/L、10μmol/L、20μmol/L和40μmol/L）处理24小时后，采用QRT-PCR技术检测细胞内炎症因子、脂肪因子及脂肪酸代谢相关因子mRNA表达水平，包括白介素-6（interleukin-6，IL-6）、肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）、瘦素（leptin）、脂联素（adiponectin）和内脂素（visfatin）以及脂肪酸合酶（fatty acid synthase，FAS）、乙酰辅酶A羧化酶（acetyl-CoA carboxylase，ACC）、脂肪组织甘油三酯水解酶（adipose triglyceride lipase，ATGL）、脂肪细胞型脂肪酸结合蛋白（adipocyte fatty acid binding protein，AFABP）。同时10μmol/L小檗碱分别处理脂肪细胞0、4、8、24、48小时后，以相同方法测定上述指标mRNA表达水平。 结果 10~40μmol/L小檗碱剂量组，脂肪细胞内IL-6、TNF-α、leptin、FAS、ATGL、AFABP mRNA表达水平均低于对照组（P<0.05），而内脂素mRNA表达较对照组升高（P<0.05），各组间脂联素mRNA表达无显著性差异（P>0.05）。8~48h时间处理组细胞内IL-6、TNF-α、leptin、AFABP、ATGL、FAS mRNA表达水平表达水平均低于对照组（P<0.05），而内脂素mRNA水平较对照组升高（P<0.05）。脂联素mRNA表达水平仅在48h处理组降低（P<0.05）。各组间ACC mRNA表达无显著性差异（P>0.05）。 结论 小檗碱能影响3T3-L1脂肪细胞炎症因子、脂肪因子及关键脂肪酶及蛋白mRNA的表达，而且呈时间、剂量依赖效应，这可能是其改善胰岛素抵抗的重要分子机制。

关键词: 小檗碱, 3T3-L1脂肪细胞, 胰岛素抵抗, 炎症因子, 脂肪因子, 脂肪酸代谢, 基因表达

Abstract: Objective To observe the effects of berberine on inflammatory factors, adipokines and fatty acid metabolism in 3T3-L1 adipocytes, and to investigate the molecular mechanism underlying berberine improving insulin resistance. Methods Inflammatory molecules , adipokines, key enzymes and protein in fatty acid metabolism mRNA in 3T3-L1 cells were determined with quantitative real time polymerase chain reaction(QRT-PCR) after treated with different concentrations of berberine (0μmol/L,5μmol/L,10μmol/L,20μmol/L and 40μmol/L) for 24 hours while treated with 10μmol/L berberine at different durations (0 h,4 h,8 h,24 h,48 h). These inflammatory factors and key enzymes in fatty acid metabolism mainly included interleukin-6 (IL-6) , tumor necrosis factor-α (TNF-α), leptin, adiponectin, visfatin, fatty acid synthase (FAS), acetyl-CoA carboxylase (ACC), adipose triglyceride lipase (ATGL), adipocyte fatty acid binding protein (AFABP). Results In 3T3-L1 adipocytes, expression of IL-6, TNF-α, leptin, FAS, ATGL, AFABP mRNA reduced with dosage at 10~40μmol/L berberine（P<0.05）while visfatin mRNA level was increased（P<0.05）. No significant difference was found in the expression of adiponectin（P>0.05）. IL-6, TNF-α, leptin, AFABP,ATGL,FAS mRNA decreased with time after 10μmol/L berberine intervention (8-48h) when compared with the control group（P<0.05） while visfatin mRNA level was increased（P<0.05）. Adiponectin mRNA was found to decreased only after treated for 48 h（P<0.05）. No significant difference was found in the expression of ACC mRNA between groups treated with berberine（P>0.05）. Conclusion Berberine has dose and time dependent effects on mRNA expression of inflammatory factors, adipokines, key enzymes and protein in fatty acid metabolism in 3T3-L1 adipocytes, suggesting the mechanisms underlying berberine improving insulin resistance.

Key words: Berberine, 3T3-L1 adipocytes, Insulin resistance, Inflammatory factors, Adipokines, Fatty acid metabolism, Gene expression