艾灸预处理对脑缺血再灌注损伤模型大鼠大脑皮质caspase-3蛋白表达的影响

艾灸预处理对脑缺血再灌注损伤模型大鼠大脑皮质caspase-3蛋白表达的影响

肖爱娇¹,康明非²,张毫¹,龚丽丽¹

1. 江西中医药大学
2. 江西中医药大学附属医院

收稿日期:2013-12-05 修回日期:2014-02-19 出版日期:2014-06-15 发布日期:2014-06-15
通讯作者: 肖爱娇

Effects of moxibustion pretreatment on the expression of caspase-3 protein in cortex of rats with cerebral ischemia-reperfusion injury

Received:2013-12-05 Revised:2014-02-19 Published:2014-06-15 Online:2014-06-15

肖爱娇1 ,康明非2 ,张毫1 ,龚丽丽1 . 艾灸预处理对脑缺血再灌注损伤模型大鼠大脑皮质caspase-3蛋白表达的影响[J]. .

摘要/Abstract

摘要： [摘要] 目的：观察艾灸预处理对大脑皮质caspase-3蛋白表达的影响，阐明艾灸预处理减轻脑缺血再灌注损伤的作用机制。方法：雄性SD大鼠21只，分为假手术组(6只)、模型组(8只)和艾灸预处理组(7只)。采用线栓法闭塞大脑中动脉2h后进行再灌注，制备局灶性脑缺血再灌注损伤大鼠模型。采用TTC染色检查脑组织梗死情况；光学显微镜观察大脑皮质的形态结构；电子显微镜观察大脑皮质神经细胞的超微结构；荧光免疫组织化学技术检测大脑皮质caspase-3蛋白的表达。结果：艾灸预处理组大鼠患侧脑组织梗死百分数[(24.9±4.7)%]低于模型组[(45.8±4.6)%]；大脑皮质神经细胞形态结构变化较模型组轻；caspase-3阳性细胞数(39.17±7.28)低于模型组(58.17±16.53)。结论：艾灸预处理具有减轻大鼠脑缺血再灌注损伤的作用，降低大脑皮质caspase-3蛋白的表达可能是其作用机理之一。

关键词: 艾灸预处理, 脑缺血再灌注损伤, 超微结构, caspase-3

Abstract: Effects of moxibustion pretreatment on the expression of caspase-3 protein in cortex of rats with cerebral ischemia-reperfusion injury XIAO Ai-jiao1,Kang Ming-fei2. ,Zhang Hao3 ,Gong Li-li4 (1.Department of Physiology;2. Department of Acupuncture and Moxibustion; 3. Graduate student, Jiangxi University of Traditional Chinese Medicine. Nanchang,330004,China) [Abstract ]Objective To observe the effects of moxibustion pretreatment on the caspase-3 expression in cortex of rats with cerebral ischemia-reperfusion injury, and the mechanism thereof. Methods Twenty-one male SD rats were randomly divided into three groups, sham group(n=6), model group(n=8) and moxibustion group(n=7). Focal cerebral ischemia-reperfusion injury rat model was induced for 2h by middle cerebral artery occlusion (MCAO) followed by reperfusion. Brain infarct was examined by TTC staining, brain morphology was with optical microscope, the ultrastructure of cell was observed with electron microscope, and the expression of caspase-3 protein in cortex was examined by immunofluorescence histochemistry. Results Percentages of infarct volume were significantly lower[(24.9±4.7)%] in rats of moxibustion pretreatment group than those of model group[(45.8±4.6)%], nerve cells experience less morphological and structural changes in moxibustion pretreatment group than those of model group, and caspase-3 positive cells(39.17±7.28) are lower in moxibustion pretreatment group than those of model group(58.17±16.53). Conclusion Moxibustion pretreatment could reduce the damage of cerebral ischemia-reperfusion, which might be through decreasing caspase-3 expression.

Key words: moxibustion pretreatment, cerebral ischemia-reperfusion injury, ultrastructure, caspase-3

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