天津医药

• 细胞与分子生物学 • 上一篇    下一篇

胶质瘤组织中TSSC1的表达及其对胶质瘤U87细胞生物学行为的影响

刘义宾,马云富,陈建设,王银辉   

  1. 河南省郑州市南阳路169号附10号郑州大学第四附属医院神经外科
  • 收稿日期:2013-10-17 修回日期:2014-01-22 出版日期:2014-06-15 发布日期:2014-06-15
  • 通讯作者: 刘义宾

Expression of TSSC1 in glioma tissue and the influence on cell biology of glioma U87 cells

  • Received:2013-10-17 Revised:2014-01-22 Published:2014-06-15 Online:2014-06-15

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摘要： 摘要 目的：对肿瘤抑制可转移候选基因1(TSSC1)在胶质瘤组织中的表达及其胶质瘤U87细胞生物学行为的影响的研究。方法：采用RT-PCR和Western blot检测80例胶质瘤原发组织和瘤旁组织中TSSC1的表达，采用MTT、迁移和侵袭实验方法检测沉默TSSC1的表达对胶质瘤细胞系U87生物学行为的影响。结果：TSSC1在胶质瘤原发瘤组织中的表达低于相应的瘤旁组织，降表达TSSC1促进胶质瘤细胞增殖、迁移和侵袭。结论：我们的结果为研究胶质细胞瘤发生和进展提供了策略。

关键词: 胶质瘤, TSSC1, 细胞生物学

Abstract: Liu YiBing1,Ma Yun Fu2,Chen Jianshe3,Wang YinHui4 Abstract Objective: To evaluate the diagnostic value of TSSC1 in glioma patients and to the role influence on cell biology of glioma U87 cells. Methods: RT-PCR and Western blot were used to examine the expression of TSSC1 in samps, including 80 normal tissues and 80 primary tumors. MTT and transwell were used to analyze the ability of proliferation, migration, and invasion in TSSC1 siRNA-transfected U87 cells. Results: TSSC1 is down-regulated in glioma and related to higher grade. Furthermore, knockdown of TSSC1 expression results in increased proliferation and invasion in U87 cells in vitro. Conclusion: Our results may provide a strategy for blocking glioma and progression.

Key words: Glioma, TSSC1, Cell Biology