天津医药

• 细胞与分子生物学 • 上一篇    下一篇

异丙酚对内毒素诱导人单核细胞系THP-1细胞TREM-1表达的影响

陆立仁1,张良清2,卢燕2   

  1. 1. 南方医科大学附属南海医院
    2. 广东医学院附属医院麻醉科
  • 收稿日期:2013-12-24 修回日期:2014-03-30 出版日期:2014-08-15 发布日期:2014-08-15
  • 通讯作者: 张良清

Effects of Propofol on Triggering Receptor Expressed on Myeloid cells-1 (TREM-1) by Lipopolysaccharide-Stimulated Human Monocyte cell lines

  • Received:2013-12-24 Revised:2014-03-30 Published:2014-08-15 Online:2014-08-15

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摘要：

【摘要】目的  探讨异丙酚对内毒素诱导人单核细胞系THP-1细胞髓样细胞触发性受体-1（TREM-1）表达的影响。方法  培养THP-1细胞，分成7组，分别对应：Control组，单纯无血清培养基培养；内毒素（LPS）组，单纯LPS100μg/L刺激；Pro组，单纯加入异丙酚100μmol/L；P1组，LPS+异丙12.5μmol/L；P2组，LPS+异丙酚25μmol/L；P3组，LPS+异丙酚50μmol/L；P4组，LPS+异丙酚100μmol/L。观察16h后收集细胞培养上清液采用ELISA测定肿瘤坏死因子（TNF）-α水平；收集细胞采用流式细胞术检测TREM-1蛋白表达水平；收集细胞采用实时定量PCR检测TREM-1mRNA表达水平。结果  与LPS组相比，P3组与P4组的细胞表面TREM-1蛋白表达水平明显增高（P<0.05），细胞上清液中TNF-α水平明显下降（P<0.05）；与Control组比较，LPS组TREM-1mRNA表达明显增加（P<0.05）；与LPS组比较，P4组细胞TREM-1mRNA表达水平降低（P<0.05）。结论  异丙酚可使LPS诱导的THP-1细胞表面TREM-1蛋白表达水平增加，细胞TNF-α的释放减少，TREM-1mRNA的表达量下降。

关键词: 内毒素类, 脂多糖类, 二异丙酚, 单核细胞, 肿瘤坏死因子α, 髓样细胞触发受体1

Abstract: [Abstract] Objective   To evaluate the effects of propofol on TREM-1 expressed on THP-1 by lipopolysaccharide-stimulated and its possible mechanisms. Methods   Cells were divided into 7 groups: one group was control, one group was exposed to LPS in final concentration of 100g/ml, one group incubated with propofol of 100μM/L, and the remaining groups were combined LPS of 100ng/ml and propofol at different dose of 12.5μM/L, 25μM/L, 50μM/L, 100μM/L respectively. Following incubation for 16 hours, concentrations of TNF-α in supernatants were measured via ELISA, TREM-1 on cells were used to undertake FACS and TREM-1 mRNA were detecetd via qRT-PCR. Results   TREM-1 on THP-1 significantly increased in group P3 and group P4 compared with group LPS (P<0.05), furthermore group P4 is higher than P3. The concentrations of TNF-α significantly (P<0.05) increased in supernatant after LPS stimulation, compared with control group, and 50μM/L or 100μM/L of propofol can significantly (P<0.05) ameliorated the concentrations of TNF-α compared with LPS-single treated group. Group P4 or group Pro has no difference (P>0.05) in TREM-1 mRNA expression, but the remaining groups significantly (P<0.05) increased the amount of TREM-1 mRNA compared with control group. Conclusions   Propofol could enhance expression of TREM-1 on surface of THP-1. Meanwhile, propofol ameliorates the concentrations of TNF-α in culture supernatant, which reduces inflammatory responses. And propofol may modulate the TREM-1 mRNA expression.

Key words: endotoxins, lipopolysaccharides, propofol, monocytes, TNF-α, Triggering receptor expressed on myeloid cells 1