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改良瑞-姬氏染色法的染色效果研究

沈兴娅   

  1. 天津市北辰区中医医院
  • 收稿日期:2014-01-14 修回日期:2014-03-16 出版日期:2014-09-15 发布日期:2014-09-15
  • 通讯作者: 沈兴娅

Study of the Effects of Improved Wright-Giemsa Staining

SHEN Xingya   

  1. Beichen District Chinese Medicine Hospital , Tianjin University of Traditional Chinese Medicine,Tianjin 300400,China
  • Received:2014-01-14 Revised:2014-03-16 Published:2014-09-15 Online:2014-09-15
  • Contact: SHEN Xingya

摘要: 【摘要】目的 研究改良瑞-姬氏染色法的染色效果。方法 采用传统与改良瑞-姬氏染色法对40例精液标本进行染色,观察改良瑞-姬氏染色对精子、精液细胞及阴道分泌物的染色效果。结果 改良瑞-姬氏染色下的中段缺陷精子率及尾部缺陷精子率分别是7.02± 2.4%和11.02±2.03%,而传统的瑞-姬氏染色下的中段缺陷精子率及尾部缺陷精子率分别是5.48±2.8%和8.05±2.56%。两方法比较差异明显(P<0.05)。两方法的正常形态精子率及头部缺陷精子率的比较无统计学差异(P>0.05)。但是染色效果不同,改良方法下精子可见明显头核的深染和顶体的突出而传统方法染色效果较模糊。改良瑞-姬氏染色下的精液细胞及阴道分泌物中细胞及滴虫等成分染色效果良好,阴道毛滴虫的鞭毛和轴柱结构尤其清晰。结论 改良瑞-姬氏染色法染色过程简单、实用,值得临床推广应用。

关键词:  改良瑞-姬氏染色法, 精子, 阴道分泌物, 滴虫, 染色效果

Abstract: [Abstract] Objective To study the effects of improved Wright-Giemsa staining. Methods To evaluate the morphological characteristics of the sperm of 40 cases of semen by both traditional and improved Wright-Giemsa staining; and to assess the staining effects of improved Wright-Giemsa staining on vaginal discharge. Results The defective rates of the middle and tail parts of sperm are respectively 7.02± 2.4% and 11.02±2.03% in improved staining group; while that is 5.48±2.8% and 8.05±2.56% in traditional staining group. There is statistical significance between these staining methods (p<0.05). There is no significant difference of the staining of normal sperm and sperm head between these two staining methods (p<0.05); however, improved staining group shows a much clear nucleus and acrosome staining than traditional methods. Improved Wright-Giemsa staining is better for sperm, vaginal discharge and Trichomonas vaginalis. More importantly, the axostyle and flagella are clearly stained with the improved methods. Conclusion Improved Wright-Giemsa staining is simple and can be used in clinical diagnosis.

Key words: Improved Wright-Giemsa staining, Sperm, Vaginal discharge, Trichomonas vaginalis, staining effect