关键词: 食管肿瘤；癌, 鳞状细胞；原癌基因蛋白质 c-met；免疫组织化学；酶联免疫吸附测定

Abstract: Abstract： Objective To explore the relationship between serum expression of c-met protein and clinic pathological features in esophageal squamous cell carcinoma (ESCC) of Kazakh and Han people. Methods A total of 50 samples of ESCC and its corresponding adjacent tissues of Kazak and Han patients were collected. The expression of c-met protein was detected by immunohistochemistry assay. The serum c-met protein was detected in 40 Kazak and Han ESCC samples and 40 Kazak and Han controls using ELISA method. Results The positive expression rates of c-met protein were higher in Kazak and Han esophageal squamous cell carcinoma patients than those in its corresponding adjacent tissues respectively. And the serum expression rates of c-met protein were higher in Kazak and Han esophageal squamous cell carcinoma patients than those of healthy controls. There were significant differences in the different tumor invasion, lymph node metastasis and TNM stage of esophageal squamous cell carcinoma tissue and serum between groups, in which the infiltrated deep muscle and se⁃ rous breakthrough (T3+T4) were higher than the infiltration of the mucosa and submucosa group (T1 +T2), with lymph node metastasis was higher than that without lymph node metastasis, and TNM stage of Ⅱ B+Ⅲ was higher than that of Ⅰ +Ⅱ A. There was no significant difference in the expression of c-met protein between ethnicity, gender, age and degree of differenti⁃ ation. Conclusion The c-met protein expression is related to the occurrence and development, the invasion, metastasis and TNM stage of ESCC in Kazak and Han nationalities. The high expression of c-met protein in tissue and serum may indicate the invasion and metastasis of esophageal cancer and prompt a late clinical stage.

Key words: esophageal neoplasm, carcinoma, squamous cell, proto-ontogeny proteins c-met, immunohistochemistry, enzyme-linked immunosorbent assay