天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (10): 1128-1132.doi: 10.11958/j.issn.0253-9896.2015.10.012

• 实验研究 • 上一篇    下一篇

腺病毒介导hBMP-2转染BMSCs复合DBM修复兔缺血性股骨头坏死的实验研究

  

  1. 桂林医学院附属医院急诊创伤外科 （邮编 541001）
  • 收稿日期:2015-05-05 修回日期:2015-07-29 出版日期:2015-10-15 发布日期:2015-10-22

Rehabilitation effect of BMSCs that was transfected with hBMP-2 through adenovirus combination with DBM on rabbit osteonecrosis in femoral head

  1. Department of Emergency Traumatic Surgery,the Affilated Hospital of Guilin Medical University,Guilin,Guangxi 541001， China
  • Received:2015-05-05 Revised:2015-07-29 Published:2015-10-15 Online:2015-10-22

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摘要：

摘要： 目的 评价人骨形态发生蛋白（hBMP） -2/骨髓间充质干细胞（BMSCs）/脱钙松质骨（DBM）对兔股骨头坏
死的修复作用， 探索临床治疗股骨头坏死的新途径。方法 髓芯减压联合液氮冰冻法制备兔股骨头坏死模型。将
造模成功兔随机分为 A、 B、 C、 D 4 组(n=12)， A 组不植入材料， 为对照组， B、 C、 D 分别植入 DBM、 DBM/BMSCs、 hBMP-
2/BMSCs/DBM。术后 4、 8 及 12 周各组分别处死 4 只， 运用 X 线技术、 大体标本观察、 HE 染色技术评判股骨头坏死
修复情况。结果 X 线示 A 组股骨头塌陷， 无明显成骨； B、 C、 D 组股骨头缺损区有骨再生现象， 但 D 组再生情况明
显优于 B、 C 组。Lane-Sandhu X 线评分 A 组<B、 C 组<D 组 （P < 0.05）， B 和 C 组差异无统计学意义。大体观示 A 组
股骨头塌陷， 钻孔存在； B、 C 组股骨头未塌陷， 钻孔存在； D 组股骨头未塌陷， 钻孔消失。HE 染色示 A 组骨小梁坏
死、 碎裂， 大量空骨陷窝； B、 C 组可见成骨细胞及新生幼稚骨小梁； D 组大量骨细胞， 新生骨小梁与正常骨小梁无异。
空骨陷窝率 A 组>B、 C 组>D 组 （P < 0.05)， B 和 C 组差异无统计学意义。结论 hBMP-2/BMSCs/DBM 植入体内后能
够诱导 BMSCs 向成骨方向分化， 对兔股骨头坏死具有较好的修复效果。

关键词: 股骨头坏死, 疾病模型, 动物, 腺病毒, 骨形态发生蛋白-2, 脱钙松质骨基, 骨髓间充质干细胞, hBMP-2/
BMSCs/DBM

Abstract:

Abstract：Objective To evaluate the effect of human bone morphogenetic protein 2 (hBMP-2)/Bone Mesenchymal
Stem Cells (BMSCs)/demineralized bone matrix(DBM) on repairing rabbits’ femoral head after necrosis and to explore the
new treatments for femoral head necrosis. Methods Femoral head necrosis models was established by clinical core decom⁃
pression combined with liquid nitrogen frozen. Then, animals were randomly devided into 4 groups (n=12 per group): Group
A were not implanted anything as control group, Group B were implanted with DBM. Group C were implanted with hBMP-2/
DBM. Group D were implanted with hBMP- 2/BMSCs/DBM. Four rabbits from each group were sacrificed at 4,8 and 12
weeks after surgery to evaluate the the repairing effect of Osteonecrosis of the femoral head (ONFH) through X-ray examina⁃
tion, observation of the specimen and HE staining. Results X-ray revealed defect of femoral head in Group A without clear
bone formation. There is a little fibrous hyperplasia and no obvious osteogenic response. By contrast, the femoral head defect
areas became fuzzy in group B, group C and group D with new bone trabeculars. And the regenerate phenomenons of group D
were significantly better than that of group B and group C of the same time point. As to the Lane-Sandhu X Ray scores, it is
lower in group A than that in group B; It is lower in group C than that in group D（P < 0.05). There is no statistical difference
between Group B and Group C. General observation of the specimen revealed that the femoral head of group A collapsed
with drilling holes. The femoral heads of group B and group C showed no collapse but the drilling holes existed. Femoral
head in group D was not collapsed and the drilling holes disappeared. HE staining showed that bone trabeculars became ne⁃
crotic and fragmented in Group A with a lot of air trapped cells. There were newborn immature bone trabeculars and osteo⁃blasts in group B and group C. Group D were of large number of bone cells, fat cells, and newborn mature bone trabeculars.
The ratio of empty lacuna is higher in Group A than that in Group B; it is higher in Group C than that in Group D（P < 0.05).
Conclusion hBMP-2/BMSCs/DBM can induce BMSCs differentiation into osteoblasts after being implanted. It has good re⁃
pairing effect on ONFH with good application prospect.

Key words: femur head necrosis, disease models, animal, adenovirus, bone morphogenetic protein -2, demineralized
bone matrix, bone marrow mesenchymal stem cells, hBMP-2/BMSCs/DBM