天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (10): 1140-1143.doi: 10.11958/j.issn.0253-9896.2015.10.015

• 实验研究 • 上一篇    下一篇

氧氟杀星明胶微球的制备工艺研究

  

  1. 齐齐哈尔大学化学与化学工程学院
  • 收稿日期:2014-11-07 修回日期:2015-05-14 出版日期:2015-10-15 发布日期:2015-10-22

Study in preparation of ofloxaxcin gelatin microspheres

  1. College of Chemistry and Chemical Engineering, Qiqihar Unversity, Qiqihar 161006, China
  • Received:2014-11-07 Revised:2015-05-14 Published:2015-10-15 Online:2015-10-22

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摘要：

摘要： 目的 研究制备氧氟沙星明胶微球的最佳工艺条件。方法 以明胶为载体， 液体石蜡为油相， Span80 为
乳化剂， 戊二醛为交联剂， 采用乳化交联法制备氧氟沙星明胶微球。采用紫外分光光度法测定微球中氧氟沙星的载
药量和包封率。考察明胶质量分数、 油水相体积比、 明胶与氧氟沙星质量比、 Span80 体积分数对载药量、 包封率的影
响。以载药量、 包封率的总和为评价指标， 针对上述 4 个因素用 L9 (34) 表正交试验设计法进行实验， 获得最佳配方比
例。结果 氧氟沙星明胶微球分别在明胶质量分数 20%、 油水相体积比为 3.5∶1、 明胶与氧氟沙星质量比 1∶1、 Span80
体积分数 2.5%时， 各自载药量、 包封率的综合评价指标较高。正交设计证实上述条件下制备工艺最佳。结论 本制备
工艺稳定可行， 氧氟沙星明胶微球的载药量和包封率可达到约 80％。

关键词: 氧氟沙星, 聚明胶肽, 微球体, 工艺学, 制药, 药物载体, 药物评价

Abstract:

Abstract： Objective To study the optimum condition of preparing ofloxaxcin gelatin microspheres. Methods Oflox⁃
axcin gelatin microspheres were manufactured using the emulsion chemical-cross linking method and gelatin was employed
as carrier, liquid paraffin as oil phase, Span80 as emulsifier, . The loading capability and entrapment efficiency of the ofloxax⁃
cin gelatin microspheres were determined by UV Spectrophotometry. The effect of gelatin concentration, oil / water volume ra⁃
tio, gelatin/ofloxaxcin mass ratio and volume fraction of span80 on drug loading capability and entrapment efficiency were in⁃
vestigated. The optimum proportions of each component was obtained by L9 (34) orthogonal test, based on the above 4 factors,
using sum of drug loading capability and entrapment efficiency as evaluation index. Results The optimum condition for
manufacturing high quality ofloxaxcin gelatin microspheres used 20% of gelatin concentration， water/oil volume ratio at 3.5∶ 1,
gelatin/ofloxaxcin mass ratio at 1∶1, the span 80 volume fraction of 2.5%. Conclusion Drug loading capability and entrap⁃
ment efficiency of the ofloxaxcin gelatin microspheres reached 80％ using this manufacture technology therefore the prepara⁃
tion was stable and feasible.

Key words: Ofloxaxcin, polygeline, microspheres, technology, pharmaceutical, drug carriers, drug evaluation