天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (10): 1152-1155.doi: 10.11958/j.issn.0253-9896.2015.10.018

• 临床研究 • 上一篇    下一篇

初治肺结核患者外周血树突状细胞免疫功能的体外研究

  

  1. 河北省石家庄市第五医院 （邮编050021）
  • 收稿日期:2014-12-30 修回日期:2015-05-12 出版日期:2015-10-15 发布日期:2015-10-22

Study of immune function of dendritic cells in peripheral blood of tuberculosis patients after initial treatment#br#

  1. The Fifth Hospital of Shijiazhuang, Shijiazhuang 050021, China
  • Received:2014-12-30 Revised:2015-05-12 Published:2015-10-15 Online:2015-10-22

PDF (PC)

3870

摘要：

摘要： 目的 研究初治肺结核患者外周血树突状细胞 （DC） 表面成熟标志的表达、 分泌细胞因子以及对 T 淋巴细
胞的刺激作用。方法 收集初治肺结核患者 68 例， 其中菌阴组 35 例， 菌阳组 33 例。收集健康体检者 40 例作为对
照组。分离外周血单个核细胞， 诱导其分化成 DC。流式细胞术检测各组 DC 的表面标志 CD83 和 CD86 的表达率；
MTT 法检测 DC 促进同种异体 T 淋巴细胞的增殖能力； ELISA 法检测 DC 培养上清中白细胞介素 （IL） -12、 IL-10、 干
扰素 （INF） -γ的含量。结果 初治肺结核患者与对照组相比， DC 表面标志 CD83 和 CD86 的表达量均显著降低 （P＜
0.05）， 刺激淋巴细胞增殖的能力均显著降低 （P＜0.05）， 培养上清中 IL-12 的含量均显著增高 （P＜0.05）， 培养上清中
IL-10、 INF-γ的含量各组间差异无统计学意义 （P＞0.05）。结论 初治肺结核患者外周血 DC 的成熟度显著下降， 刺
激混合淋巴细胞增殖的能力也显著降低， 分泌 IL-12 的能力增强。

关键词: 树突细胞, 结核, 肺, 细胞因子类, 流式细胞术

Abstract:

Abstract：Objective To investigate expressions of surface maturation markers, secreting cytokines and the stimulat⁃
ing effect on T lymphocytes in the dendritic cells (DC) from peripheral blood of tuberculosis (TB) patients after loading dose
treatment. Methods TB patients who received initial treatment (n=68) were collected at the fifth hospital of Shijiazhuang
from 2013 June to 2014 January. Base on clinical diagnosis and treatment guidelines of tuberculosis, they were divided into
sputum smear- negative group (35 cases) and sputum smear-positive group(33 cases). Forty cases of healthy adult were se⁃
lected as control group. Mononuclear cells were isolated from peripheral blood and were cultured in medium to differentiate
into DCs. Expression levels of CD83 and CD86 on DCs were examined by flow cytometry. The proliferation of allogeneic
mixed lymphocyte stimulated by DCs was dectected using MTT assay. Contents of IL-12, IL-10 and INF-γ in the cultural
supernatant of DCs and blood serum from TB patients were detected by ELISA. Results Compared with controls ,the ex⁃
pressions of CD83 and CD86 on DCs in TB patients after loading dose treatment decreased obviously（P＜0.05） , and the
ability to stimulate the proliferation of lymphocytes reduced evidently（P＜0.05） . What’ s more, IL-12 level increased mark⁃
edly （P＜0.05） while IL-10 and INF-γ levels presented no significant difference among the three groups (P＞0.05). Conclu⁃
sion The expressions of maturation markers of DC cells of the peripheral blood in TB patients after initial treatment de⁃
creased. The ability of stimulating mixed lymphocyte proliferation is also significantly reduced while secretion of IL-12 was
enhanced.

Key words: dendritic cells, tuberculosis, pulmonary, cytokines, flow cytometry